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The effect of specific rhlA-las-box mutations on DNA binding and gene activation by Pseudomonas aeruginosa quorum-sensing transcriptional regulators RhlR and LasR.
FEMS Microbiol Lett. 2014 Jul; 356(2):217-25.FM

Abstract

Pseudomonas aeruginosa is a free-living bacterium and an important opportunistic pathogen. The genes coding for virulence-associated traits are regulated at the level of transcription by the quorum-sensing response. In this response, the regulator LasR coupled with the autoinducer 3-oxo-dodecanoyl homoserine lactone (3O-C12-HSL) activates transcription of genes for several virulence factors. LasR/3O-C12-HSL also activates transcription of rhlR, the gene coding for the transcriptional regulator RhlR, and of rhlI that encodes the synthase that produces the autoinducer butanoyl-homoserine lactone (C4-HSL) that interacts with RhlR. Genes activated by RhlR/C4-HSL include those involved in rhamnolipids production (like the rhlAB operon) and lecA, coding for PA-I lectin. The molecular basis of LasR/3O-C12-HSL- and RhlR/C4-HSLDNA-binding specificity (at the so-called las-boxes) has not been clearly determined, and the aim of this work was to contribute to its understanding. Therefore, we analyzed the interaction of LasR and RhlR to variants of the rhlA-las-box that were constructed based on the comparison of this las-box to the las-box of lecA. We conclude that LasR and RhlR DNA-binding specificity is a complex multifactorial phenomenon in which both positive and negative effects are involved and that binding of these proteins does not necessarily result in gene activation.

Authors+Show Affiliations

Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, México.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Letter
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24935161

Citation

González-Valdez, Abigail, et al. "The Effect of Specific rhlA-las-box Mutations On DNA Binding and Gene Activation By Pseudomonas Aeruginosa Quorum-sensing Transcriptional Regulators RhlR and LasR." FEMS Microbiology Letters, vol. 356, no. 2, 2014, pp. 217-25.
González-Valdez A, Servín-González L, Juárez K, et al. The effect of specific rhlA-las-box mutations on DNA binding and gene activation by Pseudomonas aeruginosa quorum-sensing transcriptional regulators RhlR and LasR. FEMS Microbiol Lett. 2014;356(2):217-25.
González-Valdez, A., Servín-González, L., Juárez, K., Hernandez-Aligio, A., & Soberón-Chávez, G. (2014). The effect of specific rhlA-las-box mutations on DNA binding and gene activation by Pseudomonas aeruginosa quorum-sensing transcriptional regulators RhlR and LasR. FEMS Microbiology Letters, 356(2), 217-25. https://doi.org/10.1111/1574-6968.12505
González-Valdez A, et al. The Effect of Specific rhlA-las-box Mutations On DNA Binding and Gene Activation By Pseudomonas Aeruginosa Quorum-sensing Transcriptional Regulators RhlR and LasR. FEMS Microbiol Lett. 2014;356(2):217-25. PubMed PMID: 24935161.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of specific rhlA-las-box mutations on DNA binding and gene activation by Pseudomonas aeruginosa quorum-sensing transcriptional regulators RhlR and LasR. AU - González-Valdez,Abigail, AU - Servín-González,Luis, AU - Juárez,Katy, AU - Hernandez-Aligio,Alberto, AU - Soberón-Chávez,Gloria, PY - 2014/05/17/received PY - 2014/06/05/revised PY - 2014/06/07/accepted PY - 2014/6/18/entrez PY - 2014/6/18/pubmed PY - 2015/4/8/medline KW - consensus-sequences KW - las-box-mutations KW - protein-DNA-interaction SP - 217 EP - 25 JF - FEMS microbiology letters JO - FEMS Microbiol. Lett. VL - 356 IS - 2 N2 - Pseudomonas aeruginosa is a free-living bacterium and an important opportunistic pathogen. The genes coding for virulence-associated traits are regulated at the level of transcription by the quorum-sensing response. In this response, the regulator LasR coupled with the autoinducer 3-oxo-dodecanoyl homoserine lactone (3O-C12-HSL) activates transcription of genes for several virulence factors. LasR/3O-C12-HSL also activates transcription of rhlR, the gene coding for the transcriptional regulator RhlR, and of rhlI that encodes the synthase that produces the autoinducer butanoyl-homoserine lactone (C4-HSL) that interacts with RhlR. Genes activated by RhlR/C4-HSL include those involved in rhamnolipids production (like the rhlAB operon) and lecA, coding for PA-I lectin. The molecular basis of LasR/3O-C12-HSL- and RhlR/C4-HSLDNA-binding specificity (at the so-called las-boxes) has not been clearly determined, and the aim of this work was to contribute to its understanding. Therefore, we analyzed the interaction of LasR and RhlR to variants of the rhlA-las-box that were constructed based on the comparison of this las-box to the las-box of lecA. We conclude that LasR and RhlR DNA-binding specificity is a complex multifactorial phenomenon in which both positive and negative effects are involved and that binding of these proteins does not necessarily result in gene activation. SN - 1574-6968 UR - https://www.unboundmedicine.com/medline/citation/24935161/The_effect_of_specific_rhlA_las_box_mutations_on_DNA_binding_and_gene_activation_by_Pseudomonas_aeruginosa_quorum_sensing_transcriptional_regulators_RhlR_and_LasR_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1111/1574-6968.12505 DB - PRIME DP - Unbound Medicine ER -