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Differentiation of manuka honey from kanuka honey and from jelly bush honey using HS-SPME-GC/MS and UHPLC-PDA-MS/MS.
J Agric Food Chem. 2014 Jul 09; 62(27):6435-44.JA

Abstract

In the present study, pollen-identical pure manuka and kanuka honeys and an Australian jelly bush honey were analyzed for the nonvolatiles by UHPLC-PDA-MS/MS and for the volatiles by HS-SPME-GC/MS. A chromatographic profile matchup by means of characteristic marker compounds achieved a clear discrimination between manuka, kanuka, and jelly bush honey. UHPLC-PDA profiles of manuka honey show leptosin, acetyl-2-hydroxy-4-(2-methoxyphenyl)-4-oxobutanate, 3-hydroxy-1-(2-methoxyphenyl)-penta-1,4-dione, kojic acid, 5-methyl-3-furancarboxylic acid, and two unknown compounds as prominent, kanuka honey was characterized by 4-methoxyphenyllactic acid, methyl syringate, p-anisic acid, and lumichrome. 2-Methylbenzofuran, 2'-hydroxyacetophenone, and 2'-methoxyacetophenone were markant volatiles for manuka honey, whereas kanuka honey was characterized by 2,6,6-trimethyl-2-cyclohexene-1,4-dione, phenethyl alcohol, p-anisaldehyde, and an unknown compound in HS-SPME-GC/MS. The jelly bush honey differed from the manuka honey by higher contents of 2-methoxybenzoic acid and an individual unknown substance in the PDA profile and by lower intensities of 2'-methoxyacetophenone, higher concentrations of cis-linalool oxide, and 3,4,5-trimethylphenol in the HS-SPME-GC/MS profile.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Beitlich N
Food Chemistry Department, Technische Universität Dresden , Bergstrasse 66, 01069 Dresden, Germany.
Koelling-Speer I
No affiliation info available
Oelschlaegel S
No affiliation info available
Speer K
No affiliation info available

MeSH

AustraliaChromatography, High Pressure LiquidDiscriminant AnalysisGas Chromatography-Mass SpectrometryHoneySolid Phase MicroextractionTandem Mass SpectrometryVolatile Organic Compounds

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

24941132

Citation

Beitlich, Nicole, et al. "Differentiation of Manuka Honey From Kanuka Honey and From Jelly Bush Honey Using HS-SPME-GC/MS and UHPLC-PDA-MS/MS." Journal of Agricultural and Food Chemistry, vol. 62, no. 27, 2014, pp. 6435-44.
Beitlich N, Koelling-Speer I, Oelschlaegel S, et al. Differentiation of manuka honey from kanuka honey and from jelly bush honey using HS-SPME-GC/MS and UHPLC-PDA-MS/MS. J Agric Food Chem. 2014;62(27):6435-44.
Beitlich, N., Koelling-Speer, I., Oelschlaegel, S., & Speer, K. (2014). Differentiation of manuka honey from kanuka honey and from jelly bush honey using HS-SPME-GC/MS and UHPLC-PDA-MS/MS. Journal of Agricultural and Food Chemistry, 62(27), 6435-44. https://doi.org/10.1021/jf501818f
Beitlich N, et al. Differentiation of Manuka Honey From Kanuka Honey and From Jelly Bush Honey Using HS-SPME-GC/MS and UHPLC-PDA-MS/MS. J Agric Food Chem. 2014 Jul 9;62(27):6435-44. PubMed PMID: 24941132.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differentiation of manuka honey from kanuka honey and from jelly bush honey using HS-SPME-GC/MS and UHPLC-PDA-MS/MS. AU - Beitlich,Nicole, AU - Koelling-Speer,Isabelle, AU - Oelschlaegel,Stefanie, AU - Speer,Karl, Y1 - 2014/06/26/ PY - 2014/6/19/entrez PY - 2014/6/19/pubmed PY - 2015/2/3/medline SP - 6435 EP - 44 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 62 IS - 27 N2 - In the present study, pollen-identical pure manuka and kanuka honeys and an Australian jelly bush honey were analyzed for the nonvolatiles by UHPLC-PDA-MS/MS and for the volatiles by HS-SPME-GC/MS. A chromatographic profile matchup by means of characteristic marker compounds achieved a clear discrimination between manuka, kanuka, and jelly bush honey. UHPLC-PDA profiles of manuka honey show leptosin, acetyl-2-hydroxy-4-(2-methoxyphenyl)-4-oxobutanate, 3-hydroxy-1-(2-methoxyphenyl)-penta-1,4-dione, kojic acid, 5-methyl-3-furancarboxylic acid, and two unknown compounds as prominent, kanuka honey was characterized by 4-methoxyphenyllactic acid, methyl syringate, p-anisic acid, and lumichrome. 2-Methylbenzofuran, 2'-hydroxyacetophenone, and 2'-methoxyacetophenone were markant volatiles for manuka honey, whereas kanuka honey was characterized by 2,6,6-trimethyl-2-cyclohexene-1,4-dione, phenethyl alcohol, p-anisaldehyde, and an unknown compound in HS-SPME-GC/MS. The jelly bush honey differed from the manuka honey by higher contents of 2-methoxybenzoic acid and an individual unknown substance in the PDA profile and by lower intensities of 2'-methoxyacetophenone, higher concentrations of cis-linalool oxide, and 3,4,5-trimethylphenol in the HS-SPME-GC/MS profile. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/24941132/Differentiation_of_manuka_honey_from_kanuka_honey_and_from_jelly_bush_honey_using_HS_SPME_GC/MS_and_UHPLC_PDA_MS/MS_ DB - PRIME DP - Unbound Medicine ER -