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[Effect of Moutan Cortex on AGEs-induced mesangial cell proliferation and basement membrane thickening].
Zhongguo Zhong Yao Za Zhi. 2014 Feb; 39(3):478-82.ZZ

Abstract

OBJECTIVE

To investigate the effect of Moutan Cortex on mesangial proliferation and basement membrane thickening induced by advanced glycation end products (AGEs).

METHOD

The glomerular mesangial cells (MC) injury model was established by inducing by AGEs. The cell were divided into 6 groups: the blank group (BSA, 200 mg L-1) , the model group (AGEs, 200 mg L-1), the positive control group (AG, 10 mmol L L-1), and drug administration groups, namely the Moutan Cortex-treated high-dose group (2 x 10(-4) g mL(- 1)), the Moutan Cortex-treated medium-dose group (1 x 10(-4) g mL-1), and the Moutan Cortex-treated low-dose group (0. 5 x 10(-4) g . mL(-1)). The MTT method was performed to observe the effect of Moutan Cortex on the proliferation of MC. The content of fibronectin (FN) and collagen secretion 1V (Col IV) in cell supernatant were detected by ELISA kits. The western blot analysis was carried out to observe the FN expression. The Real-time PCR analysis was applied to examine the Col IV mRNA expression.

RESULT

AGEs significantly increased AGEs-induced MC proliferation and FN and Col 1V secretion. The western blot analysis showed that MC could down-regulate the FN expression of MC secretion. According to the results of the real-time PCR assay, MC could down-regulate AGEs-induced MC secretion Col IV mRNA expression.

CONCLUSION

MC had a certain protective effect on MC cultured under AGEs conditions. MC could remarkably inhibit the composition and secretion of Col IV and FN in matrix and the basement membrane thickening, and provide an experimental basis for the treatment of diabetic nephropathy.

Authors

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Pub Type(s)

Journal Article

Language

chi

PubMed ID

24946551

Citation

Zhang, Ming-Hua, et al. "[Effect of Moutan Cortex On AGEs-induced Mesangial Cell Proliferation and Basement Membrane Thickening]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 39, no. 3, 2014, pp. 478-82.
Zhang MH, Feng L, Gun JF, et al. [Effect of Moutan Cortex on AGEs-induced mesangial cell proliferation and basement membrane thickening]. Zhongguo Zhong Yao Za Zhi. 2014;39(3):478-82.
Zhang, M. H., Feng, L., Gun, J. F., Jiang, J., & Jia, X. B. (2014). [Effect of Moutan Cortex on AGEs-induced mesangial cell proliferation and basement membrane thickening]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 39(3), 478-82.
Zhang MH, et al. [Effect of Moutan Cortex On AGEs-induced Mesangial Cell Proliferation and Basement Membrane Thickening]. Zhongguo Zhong Yao Za Zhi. 2014;39(3):478-82. PubMed PMID: 24946551.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Moutan Cortex on AGEs-induced mesangial cell proliferation and basement membrane thickening]. AU - Zhang,Ming-Hua, AU - Feng,Liang, AU - Gun,Jun-Fei, AU - Jiang,Jun, AU - Jia,Xiao-Bin, PY - 2014/6/21/entrez PY - 2014/6/21/pubmed PY - 2014/7/11/medline SP - 478 EP - 82 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 39 IS - 3 N2 - OBJECTIVE: To investigate the effect of Moutan Cortex on mesangial proliferation and basement membrane thickening induced by advanced glycation end products (AGEs). METHOD: The glomerular mesangial cells (MC) injury model was established by inducing by AGEs. The cell were divided into 6 groups: the blank group (BSA, 200 mg L-1) , the model group (AGEs, 200 mg L-1), the positive control group (AG, 10 mmol L L-1), and drug administration groups, namely the Moutan Cortex-treated high-dose group (2 x 10(-4) g mL(- 1)), the Moutan Cortex-treated medium-dose group (1 x 10(-4) g mL-1), and the Moutan Cortex-treated low-dose group (0. 5 x 10(-4) g . mL(-1)). The MTT method was performed to observe the effect of Moutan Cortex on the proliferation of MC. The content of fibronectin (FN) and collagen secretion 1V (Col IV) in cell supernatant were detected by ELISA kits. The western blot analysis was carried out to observe the FN expression. The Real-time PCR analysis was applied to examine the Col IV mRNA expression. RESULT: AGEs significantly increased AGEs-induced MC proliferation and FN and Col 1V secretion. The western blot analysis showed that MC could down-regulate the FN expression of MC secretion. According to the results of the real-time PCR assay, MC could down-regulate AGEs-induced MC secretion Col IV mRNA expression. CONCLUSION: MC had a certain protective effect on MC cultured under AGEs conditions. MC could remarkably inhibit the composition and secretion of Col IV and FN in matrix and the basement membrane thickening, and provide an experimental basis for the treatment of diabetic nephropathy. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/24946551/[Effect_of_Moutan_Cortex_on_AGEs_induced_mesangial_cell_proliferation_and_basement_membrane_thickening]_ DB - PRIME DP - Unbound Medicine ER -