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Simultaneous concentration and detection of biomarkers on paper.
Lab Chip. 2014 Aug 21; 14(16):3021-8.LC

Abstract

The lateral-flow immunoassay (LFA) is an inexpensive point-of-care (POC) paper-based diagnostic device with the potential to rapidly detect disease biomarkers in resource-poor settings. Although LFA is inexpensive, easy to use, and requires no laboratory equipment, it is limited by its sensitivity, which remains inferior to that of gold standard laboratory-based assays. Our group is the only one to have previously utilized various aqueous two-phase systems (ATPSs) to enhance LFA detection. In those studies, the sample was concentrated by an ATPS in a test tube and could only be applied to LFA after it had been extracted manually. Here, we bypass the extraction step by seamlessly integrating a polyethylene glycol-potassium phosphate ATPS with downstream LFA detection in a simple, inexpensive, power-free, and portable all-in-one diagnostic device. We discovered a new phenomenon in which the target biomarkers simultaneously concentrate as the ATPS solution flows through the paper membranes, and our device features a 3-D paper well that was designed to exploit this phenomenon. Studies with this device, which were performed at room temperature in under 25 min, demonstrated a 10-fold improvement in the detection limit of a model protein, transferrin. Our next-generation LFA technology is rapid, affordable, easy-to-use, and can be applied to existing LFA products, thereby providing a new platform for revolutionizing the current state of disease diagnosis in resource-poor settings.

Authors+Show Affiliations

Department of Bioengineering, University of California, 420 Westwood Plaza, 5121J Engineering V, P.O. Box 951600, Los Angeles, CA 90095-1600, USA. kamei@seas.ucla.edu.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24950897

Citation

Chiu, Ricky Y T., et al. "Simultaneous Concentration and Detection of Biomarkers On Paper." Lab On a Chip, vol. 14, no. 16, 2014, pp. 3021-8.
Chiu RY, Jue E, Yip AT, et al. Simultaneous concentration and detection of biomarkers on paper. Lab Chip. 2014;14(16):3021-8.
Chiu, R. Y., Jue, E., Yip, A. T., Berg, A. R., Wang, S. J., Kivnick, A. R., Nguyen, P. T., & Kamei, D. T. (2014). Simultaneous concentration and detection of biomarkers on paper. Lab On a Chip, 14(16), 3021-8. https://doi.org/10.1039/c4lc00532e
Chiu RY, et al. Simultaneous Concentration and Detection of Biomarkers On Paper. Lab Chip. 2014 Aug 21;14(16):3021-8. PubMed PMID: 24950897.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous concentration and detection of biomarkers on paper. AU - Chiu,Ricky Y T, AU - Jue,Erik, AU - Yip,Allison T, AU - Berg,Andrew R, AU - Wang,Stephanie J, AU - Kivnick,Alexandra R, AU - Nguyen,Phuong T, AU - Kamei,Daniel T, Y1 - 2014/06/20/ PY - 2014/6/22/entrez PY - 2014/6/22/pubmed PY - 2015/5/12/medline SP - 3021 EP - 8 JF - Lab on a chip JO - Lab Chip VL - 14 IS - 16 N2 - The lateral-flow immunoassay (LFA) is an inexpensive point-of-care (POC) paper-based diagnostic device with the potential to rapidly detect disease biomarkers in resource-poor settings. Although LFA is inexpensive, easy to use, and requires no laboratory equipment, it is limited by its sensitivity, which remains inferior to that of gold standard laboratory-based assays. Our group is the only one to have previously utilized various aqueous two-phase systems (ATPSs) to enhance LFA detection. In those studies, the sample was concentrated by an ATPS in a test tube and could only be applied to LFA after it had been extracted manually. Here, we bypass the extraction step by seamlessly integrating a polyethylene glycol-potassium phosphate ATPS with downstream LFA detection in a simple, inexpensive, power-free, and portable all-in-one diagnostic device. We discovered a new phenomenon in which the target biomarkers simultaneously concentrate as the ATPS solution flows through the paper membranes, and our device features a 3-D paper well that was designed to exploit this phenomenon. Studies with this device, which were performed at room temperature in under 25 min, demonstrated a 10-fold improvement in the detection limit of a model protein, transferrin. Our next-generation LFA technology is rapid, affordable, easy-to-use, and can be applied to existing LFA products, thereby providing a new platform for revolutionizing the current state of disease diagnosis in resource-poor settings. SN - 1473-0189 UR - https://www.unboundmedicine.com/medline/citation/24950897/Simultaneous_concentration_and_detection_of_biomarkers_on_paper_ L2 - https://doi.org/10.1039/c4lc00532e DB - PRIME DP - Unbound Medicine ER -