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Enhanced freeze tolerance of baker's yeast by overexpressed trehalose-6-phosphate synthase gene (TPS1) and deleted trehalase genes in frozen dough.
J Ind Microbiol Biotechnol 2014; 41(8):1275-85JI

Abstract

Several recombinant strains with overexpressed trehalose-6-phosphate synthase gene (TPS1) and/or deleted trehalase genes were obtained to elucidate the relationships between TPS1, trehalase genes, content of intracellular trehalose and freeze tolerance of baker's yeast, as well as improve the fermentation properties of lean dough after freezing. In this study, strain TL301(TPS1) overexpressing TPS1 showed 62.92 % higher trehalose-6-phosphate synthase (Tps1) activity and enhanced the content of intracellular trehalose than the parental strain. Deleting ATH1 exerted a significant effect on trehalase activities and the degradation amount of intracellular trehalose during the first 30 min of prefermentation. This finding indicates that acid trehalase (Ath1) plays a role in intracellular trehalose degradation. NTH2 encodes a functional neutral trehalase (Nth2) that was significantly involved in intracellular trehalose degradation in the absence of the NTH1 and/or ATH1 gene. The survival ratio, freeze-tolerance ratio and relative fermentation ability of strain TL301(TPS1) were approximately twice as high as those of the parental strain (BY6-9α). The increase in freeze tolerance of strain TL301(TPS1) was accompanied by relatively low trehalase activity, high Tps1 activity and high residual content of intracellular trehalose. Our results suggest that overexpressing TPS1 and deleting trehalase genes are sufficient to improve the freeze tolerance of baker's yeast in frozen dough. The present study provides guidance for the commercial baking industry as well as the research on the intracellular trehalose mobilization and freeze tolerance of baker's yeast.

Authors+Show Affiliations

Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China, haigang1998@163.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24951963

Citation

Tan, Haigang, et al. "Enhanced Freeze Tolerance of Baker's Yeast By Overexpressed Trehalose-6-phosphate Synthase Gene (TPS1) and Deleted Trehalase Genes in Frozen Dough." Journal of Industrial Microbiology & Biotechnology, vol. 41, no. 8, 2014, pp. 1275-85.
Tan H, Dong J, Wang G, et al. Enhanced freeze tolerance of baker's yeast by overexpressed trehalose-6-phosphate synthase gene (TPS1) and deleted trehalase genes in frozen dough. J Ind Microbiol Biotechnol. 2014;41(8):1275-85.
Tan, H., Dong, J., Wang, G., Xu, H., Zhang, C., & Xiao, D. (2014). Enhanced freeze tolerance of baker's yeast by overexpressed trehalose-6-phosphate synthase gene (TPS1) and deleted trehalase genes in frozen dough. Journal of Industrial Microbiology & Biotechnology, 41(8), pp. 1275-85. doi:10.1007/s10295-014-1467-7.
Tan H, et al. Enhanced Freeze Tolerance of Baker's Yeast By Overexpressed Trehalose-6-phosphate Synthase Gene (TPS1) and Deleted Trehalase Genes in Frozen Dough. J Ind Microbiol Biotechnol. 2014;41(8):1275-85. PubMed PMID: 24951963.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced freeze tolerance of baker's yeast by overexpressed trehalose-6-phosphate synthase gene (TPS1) and deleted trehalase genes in frozen dough. AU - Tan,Haigang, AU - Dong,Jian, AU - Wang,Guanglu, AU - Xu,Haiyan, AU - Zhang,Cuiying, AU - Xiao,Dongguang, Y1 - 2014/06/21/ PY - 2014/03/26/received PY - 2014/05/22/accepted PY - 2014/6/22/entrez PY - 2014/6/22/pubmed PY - 2014/12/15/medline SP - 1275 EP - 85 JF - Journal of industrial microbiology & biotechnology JO - J. Ind. Microbiol. Biotechnol. VL - 41 IS - 8 N2 - Several recombinant strains with overexpressed trehalose-6-phosphate synthase gene (TPS1) and/or deleted trehalase genes were obtained to elucidate the relationships between TPS1, trehalase genes, content of intracellular trehalose and freeze tolerance of baker's yeast, as well as improve the fermentation properties of lean dough after freezing. In this study, strain TL301(TPS1) overexpressing TPS1 showed 62.92 % higher trehalose-6-phosphate synthase (Tps1) activity and enhanced the content of intracellular trehalose than the parental strain. Deleting ATH1 exerted a significant effect on trehalase activities and the degradation amount of intracellular trehalose during the first 30 min of prefermentation. This finding indicates that acid trehalase (Ath1) plays a role in intracellular trehalose degradation. NTH2 encodes a functional neutral trehalase (Nth2) that was significantly involved in intracellular trehalose degradation in the absence of the NTH1 and/or ATH1 gene. The survival ratio, freeze-tolerance ratio and relative fermentation ability of strain TL301(TPS1) were approximately twice as high as those of the parental strain (BY6-9α). The increase in freeze tolerance of strain TL301(TPS1) was accompanied by relatively low trehalase activity, high Tps1 activity and high residual content of intracellular trehalose. Our results suggest that overexpressing TPS1 and deleting trehalase genes are sufficient to improve the freeze tolerance of baker's yeast in frozen dough. The present study provides guidance for the commercial baking industry as well as the research on the intracellular trehalose mobilization and freeze tolerance of baker's yeast. SN - 1476-5535 UR - https://www.unboundmedicine.com/medline/citation/24951963/Enhanced_freeze_tolerance_of_baker's_yeast_by_overexpressed_trehalose_6_phosphate_synthase_gene__TPS1__and_deleted_trehalase_genes_in_frozen_dough_ L2 - http://dx.doi.org/10.1007/s10295-014-1467-7 DB - PRIME DP - Unbound Medicine ER -