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Covalent functionalization of gold nanoparticles as electronic bridges and signal amplifiers towards an electrochemical immunosensor for botulinum neurotoxin type A.
Biosens Bioelectron 2014; 61:547-53BB

Abstract

This work introduced an efficient approach for modification of AuNPs with multicomponents by diazonium salt couplings. The multifunctionalized AuNPs with protruding functional groups that allow simple bioconjugation to large amounts of biomolecules have been successfully used as electronic bridges and signal amplifiers for an electrochemical immunosensor towards the detection of BoNT/A. The one-step anchoring AuNPs strategy has greatly increased the efficiency for attachment of biomolecules and subsequently increased the sensitivity. Sensitivity was further amplified by preparation of bioconjugates particles containing horseradish peroxidase (HRP) labels along with detection antibodies (AbL) attached to AuNPs. The immunosensor can be used for the detection of BoNT/A over the range of 4-35 pg mL(-1) with the lowest detection limit of 1 pg mL(-1) and assay time of 10 min. The herein sensing strategy is rapid, robust, selective, sensitive, and is promising for future fabrication of point-of-care devices.

Authors+Show Affiliations

Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China. Electronic address: gzliu@mail.ccnu.edu.cn.Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China.Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China.

Pub Type(s)

Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24953841

Citation

Liu, Guozhen, et al. "Covalent Functionalization of Gold Nanoparticles as Electronic Bridges and Signal Amplifiers Towards an Electrochemical Immunosensor for Botulinum Neurotoxin Type A." Biosensors & Bioelectronics, vol. 61, 2014, pp. 547-53.
Liu G, Zhang Y, Guo W. Covalent functionalization of gold nanoparticles as electronic bridges and signal amplifiers towards an electrochemical immunosensor for botulinum neurotoxin type A. Biosens Bioelectron. 2014;61:547-53.
Liu, G., Zhang, Y., & Guo, W. (2014). Covalent functionalization of gold nanoparticles as electronic bridges and signal amplifiers towards an electrochemical immunosensor for botulinum neurotoxin type A. Biosensors & Bioelectronics, 61, pp. 547-53. doi:10.1016/j.bios.2014.06.001.
Liu G, Zhang Y, Guo W. Covalent Functionalization of Gold Nanoparticles as Electronic Bridges and Signal Amplifiers Towards an Electrochemical Immunosensor for Botulinum Neurotoxin Type A. Biosens Bioelectron. 2014 Nov 15;61:547-53. PubMed PMID: 24953841.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Covalent functionalization of gold nanoparticles as electronic bridges and signal amplifiers towards an electrochemical immunosensor for botulinum neurotoxin type A. AU - Liu,Guozhen, AU - Zhang,Yin, AU - Guo,Wenqi, Y1 - 2014/06/05/ PY - 2014/05/12/received PY - 2014/06/02/revised PY - 2014/06/02/accepted PY - 2014/6/24/entrez PY - 2014/6/24/pubmed PY - 2015/2/24/medline KW - AuNP KW - Au–C bonding KW - BoNT/A KW - Immunosensor KW - Signal amplification SP - 547 EP - 53 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 61 N2 - This work introduced an efficient approach for modification of AuNPs with multicomponents by diazonium salt couplings. The multifunctionalized AuNPs with protruding functional groups that allow simple bioconjugation to large amounts of biomolecules have been successfully used as electronic bridges and signal amplifiers for an electrochemical immunosensor towards the detection of BoNT/A. The one-step anchoring AuNPs strategy has greatly increased the efficiency for attachment of biomolecules and subsequently increased the sensitivity. Sensitivity was further amplified by preparation of bioconjugates particles containing horseradish peroxidase (HRP) labels along with detection antibodies (AbL) attached to AuNPs. The immunosensor can be used for the detection of BoNT/A over the range of 4-35 pg mL(-1) with the lowest detection limit of 1 pg mL(-1) and assay time of 10 min. The herein sensing strategy is rapid, robust, selective, sensitive, and is promising for future fabrication of point-of-care devices. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/24953841/Covalent_functionalization_of_gold_nanoparticles_as_electronic_bridges_and_signal_amplifiers_towards_an_electrochemical_immunosensor_for_botulinum_neurotoxin_type_A_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(14)00399-6 DB - PRIME DP - Unbound Medicine ER -