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Identification of caleosin and two oleosin isoforms in oil bodies of pine megagametophytes.
Plant Physiol Biochem. 2014 Sep; 82:142-50.PP

Abstract

Numerous oil bodies of 0.2-2 μm occupied approximately 80% of intracellular space in mature pine (Pinus massoniana) megagametophytes. They were stably isolated and found to comprise mostly triacylglycerols as examined by thin layer chromatography analysis and confirmed by both Nile red and BODIPY stainings. Fatty acids released from the triacylglycerols of pine oil bodies were mainly unsaturated, including linoleic acid (60%), adrenic acid (12.3%) and vaccenic acid (9.7%). Proteins extracted from pine oil bodies were subjected to immunological cross-recognition, and the results showed that three proteins of 28, 16 and 14 kDa were detected by antibodies against sesame seed caleosin, sesame oleosin-L and lily pollen oleosin-P, respectively. Complete cDNA fragments encoding these three pine oil-body proteins, tentatively named caleosin, oleosin-L and oleosin-G, were obtained by PCR cloning and further confirmed by mass spectrometric analysis. Consistently, phylogenetic tree analyses showed that pine caleosin was closely-related to the caleosin of cycad megagametophyte among known caleosin sequences. While pine oleosin-L was found clustered with seed oleosin isoforms of angiosperm species, oleosin-G was distinctively grouped with the oleosin-P of lily pollen. The oleosin-G identified in pine megagametophytes seems to represent a new class of seed oleosin isoform evolutionarily close to the pollen oleosin-P.

Authors+Show Affiliations

Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan.Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan.Taiwan Coral Research Center, National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan; Institute of Marine Biotechnology, National Dong-Hwa University, Pingtung 944, Taiwan.College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.Taiwan Coral Research Center, National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan; Institute of Marine Biotechnology, National Dong-Hwa University, Pingtung 944, Taiwan. Electronic address: villy@nmmba.gov.tw.Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan; Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan. Electronic address: TCTZEN@dragon.nchu.edu.tw.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24954070

Citation

Pasaribu, Buntora, et al. "Identification of Caleosin and Two Oleosin Isoforms in Oil Bodies of Pine Megagametophytes." Plant Physiology and Biochemistry : PPB, vol. 82, 2014, pp. 142-50.
Pasaribu B, Chung TY, Chen CS, et al. Identification of caleosin and two oleosin isoforms in oil bodies of pine megagametophytes. Plant Physiol Biochem. 2014;82:142-50.
Pasaribu, B., Chung, T. Y., Chen, C. S., Wang, S. L., Jiang, P. L., & Tzen, J. T. (2014). Identification of caleosin and two oleosin isoforms in oil bodies of pine megagametophytes. Plant Physiology and Biochemistry : PPB, 82, 142-50. https://doi.org/10.1016/j.plaphy.2014.05.015
Pasaribu B, et al. Identification of Caleosin and Two Oleosin Isoforms in Oil Bodies of Pine Megagametophytes. Plant Physiol Biochem. 2014;82:142-50. PubMed PMID: 24954070.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of caleosin and two oleosin isoforms in oil bodies of pine megagametophytes. AU - Pasaribu,Buntora, AU - Chung,Tse-Yu, AU - Chen,Chii-Shiarng, AU - Wang,Song-Liang, AU - Jiang,Pei-Luen, AU - Tzen,Jason T C, Y1 - 2014/06/05/ PY - 2014/04/25/received PY - 2014/05/27/accepted PY - 2014/6/24/entrez PY - 2014/6/24/pubmed PY - 2015/3/31/medline KW - Caleosin KW - Oil body KW - Oleosin KW - Pine KW - Pollen SP - 142 EP - 50 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 82 N2 - Numerous oil bodies of 0.2-2 μm occupied approximately 80% of intracellular space in mature pine (Pinus massoniana) megagametophytes. They were stably isolated and found to comprise mostly triacylglycerols as examined by thin layer chromatography analysis and confirmed by both Nile red and BODIPY stainings. Fatty acids released from the triacylglycerols of pine oil bodies were mainly unsaturated, including linoleic acid (60%), adrenic acid (12.3%) and vaccenic acid (9.7%). Proteins extracted from pine oil bodies were subjected to immunological cross-recognition, and the results showed that three proteins of 28, 16 and 14 kDa were detected by antibodies against sesame seed caleosin, sesame oleosin-L and lily pollen oleosin-P, respectively. Complete cDNA fragments encoding these three pine oil-body proteins, tentatively named caleosin, oleosin-L and oleosin-G, were obtained by PCR cloning and further confirmed by mass spectrometric analysis. Consistently, phylogenetic tree analyses showed that pine caleosin was closely-related to the caleosin of cycad megagametophyte among known caleosin sequences. While pine oleosin-L was found clustered with seed oleosin isoforms of angiosperm species, oleosin-G was distinctively grouped with the oleosin-P of lily pollen. The oleosin-G identified in pine megagametophytes seems to represent a new class of seed oleosin isoform evolutionarily close to the pollen oleosin-P. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/24954070/Identification_of_caleosin_and_two_oleosin_isoforms_in_oil_bodies_of_pine_megagametophytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(14)00180-6 DB - PRIME DP - Unbound Medicine ER -