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Micro-computed tomography assessment of vertebral column defects in retinoic acid-induced rat model of myelomeningocele.

Abstract

BACKGROUND

Myelomeningocele (MMC) is a common congenital malformation and the most severe form of spina bifida characterized by the protrusion of spinal cord and meninges through the spinal defect. Our objective was to improve the assessment of congenital vertebral defects in animal models of MMC using three-dimensional high resolution micro-computed tomography (micro-CT) imaging and quantitative digital analyses methods.

METHODS

Lumbosacral MMC was induced in fetal rats by exposure of pregnant mothers at embryonic day 10 (E10) to all-trans retinoic acid, and rats were examined at term (embryonic day 22). The axial skeleton was examined in an MMC model for the first time using ex vivo micro-CT at 10 μm voxel resolution to allow high resolution two-dimensional and three-dimensional characterization of anomalies in lumbosacral vertebrae, and quantitative assessment of distances between dorsal vertebral arches in lumbosacral regions in MMC rats, compared with normal controls.

RESULTS

We observed, in detail, skeletal defects in lumbosacral vertebra of MMC rats, including in the morphology of individual dorsal vertebral arches. Use of high resolution micro-CT has also enabled us to identify the delayed (nonfused) or absent ossification in vertebral bodies, increased fusion of adjacent lateral vertebral elements, and quantify the extent of dorsal arch widening. Distances between dorsal vertebral arches showed statistically significant increases from L5 through S4 in MMC rats, compared with normal controls.

CONCLUSION

High-resolution micro-CT combined with digital quantification methods is a powerful technique ideally suited for precise assessment of complex congenital skeletal abnormalities such as examined in this rodent model of MMC.

Authors+Show Affiliations

Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, Pennsylvania.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24954432

Citation

Barbe, Mary F., et al. "Micro-computed Tomography Assessment of Vertebral Column Defects in Retinoic Acid-induced Rat Model of Myelomeningocele." Birth Defects Research. Part A, Clinical and Molecular Teratology, vol. 100, no. 6, 2014, pp. 453-62.
Barbe MF, Adiga R, Gordiienko O, et al. Micro-computed tomography assessment of vertebral column defects in retinoic acid-induced rat model of myelomeningocele. Birth Defects Res Part A Clin Mol Teratol. 2014;100(6):453-62.
Barbe, M. F., Adiga, R., Gordiienko, O., Pleshko, N., Selzer, M. E., & Krynska, B. (2014). Micro-computed tomography assessment of vertebral column defects in retinoic acid-induced rat model of myelomeningocele. Birth Defects Research. Part A, Clinical and Molecular Teratology, 100(6), pp. 453-62. doi:10.1002/bdra.23254.
Barbe MF, et al. Micro-computed Tomography Assessment of Vertebral Column Defects in Retinoic Acid-induced Rat Model of Myelomeningocele. Birth Defects Res Part A Clin Mol Teratol. 2014;100(6):453-62. PubMed PMID: 24954432.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Micro-computed tomography assessment of vertebral column defects in retinoic acid-induced rat model of myelomeningocele. AU - Barbe,Mary F, AU - Adiga,Radhika, AU - Gordiienko,Oleg, AU - Pleshko,Nancy, AU - Selzer,Michael E, AU - Krynska,Barbara, PY - 2013/10/09/received PY - 2014/04/08/revised PY - 2014/04/22/accepted PY - 2014/6/24/entrez PY - 2014/6/24/pubmed PY - 2015/2/3/medline KW - birth defects KW - bone defects KW - myelomeningocele KW - retinoic acid KW - spina bifida SP - 453 EP - 62 JF - Birth defects research. Part A, Clinical and molecular teratology JO - Birth Defects Res. Part A Clin. Mol. Teratol. VL - 100 IS - 6 N2 - BACKGROUND: Myelomeningocele (MMC) is a common congenital malformation and the most severe form of spina bifida characterized by the protrusion of spinal cord and meninges through the spinal defect. Our objective was to improve the assessment of congenital vertebral defects in animal models of MMC using three-dimensional high resolution micro-computed tomography (micro-CT) imaging and quantitative digital analyses methods. METHODS: Lumbosacral MMC was induced in fetal rats by exposure of pregnant mothers at embryonic day 10 (E10) to all-trans retinoic acid, and rats were examined at term (embryonic day 22). The axial skeleton was examined in an MMC model for the first time using ex vivo micro-CT at 10 μm voxel resolution to allow high resolution two-dimensional and three-dimensional characterization of anomalies in lumbosacral vertebrae, and quantitative assessment of distances between dorsal vertebral arches in lumbosacral regions in MMC rats, compared with normal controls. RESULTS: We observed, in detail, skeletal defects in lumbosacral vertebra of MMC rats, including in the morphology of individual dorsal vertebral arches. Use of high resolution micro-CT has also enabled us to identify the delayed (nonfused) or absent ossification in vertebral bodies, increased fusion of adjacent lateral vertebral elements, and quantify the extent of dorsal arch widening. Distances between dorsal vertebral arches showed statistically significant increases from L5 through S4 in MMC rats, compared with normal controls. CONCLUSION: High-resolution micro-CT combined with digital quantification methods is a powerful technique ideally suited for precise assessment of complex congenital skeletal abnormalities such as examined in this rodent model of MMC. SN - 1542-0760 UR - https://www.unboundmedicine.com/medline/citation/24954432/Micro_computed_tomography_assessment_of_vertebral_column_defects_in_retinoic_acid_induced_rat_model_of_myelomeningocele_ L2 - https://doi.org/10.1002/bdra.23254 DB - PRIME DP - Unbound Medicine ER -