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Antitubercular activity of Arctium lappa and Tussilago farfara extracts and constituents.
J Ethnopharmacol. 2014 Aug 08; 155(1):796-800.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Arctium lappa and Tussilago farfara (Asteraceae) are two plant species used traditionally as antitubercular remedies. The aim of this study was (i) to screen Arctium lappa and Tussilago farfara extracts for activity against Mycobacterium tuberculosis and (ii) to isolate and identify the compound(s) responsible for this reputed anti-TB effect.

MATERIALS AND METHODS

The activity of extracts and isolated compounds was determined against Mycobacterium tuberculosis H37Rv using a high throughput spot culture growth inhibition (HT-SPOTi) assay.

RESULTS

The n-hexane extracts of both plants, the ethyl acetate extract of Tussilago farfara and the dichloromethane phase derived from the methanol extract of Arctium lappa displayed antitubercular activity (MIC 62.5 μg/mL). Further chemical investigation of Arctium lappa led to the isolation of n-nonacosane (1), taraxasterol acetate (2), taraxasterol (3), a (1:1) mixture of β sitosterol/stigmasterol (4), isololiolide (5), melitensin (6), trans-caffeic acid (7), kaempferol (8), quercetin (9), kaempferol-3-O-glucoside (10). Compounds isolated from Tussilago farfara were identified as a (1:1) mixture of β sitosterol/stigmasterol (4), trans-caffeic acid (7), kaempferol (8), quercetin (9), kaempferol-3-O-glucoside (10), loliolide (11), a (4:1) mixture of p-coumaric acid/4-hydroxybenzoic acid (12), p-coumaric acid (13). All compounds were identified following analyses of their physicochemical and spectroscopic data (MS, (1)H and (13)C-NMR) and by comparison with published data. This is the first report of the isolation of n-nonacosane (1), isololiolide (5), melitensin (6) and kaempferol-3-O-glucoside (10) from Arctium lappa, and of loliolide (11) from Tussilago farfara. Amongst the isolated compounds, the best activity was observed for p-coumaric acid (13) (MIC 31.3 μg/mL or 190.9 μM) alone and in mixture with 4-hydroxybenzoic acid (12) (MIC 62.5 μg/mL).

CONCLUSIONS

The above results provide for the first time some scientific evidence to support, to some extent, the ethno-medicinal use of Arctium lappa and Tussilago farfara as traditional antitubercular remedies.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Zhao J
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.
Evangelopoulos D
Mycobacteria Research Laboratory, Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck, University of London, London, UK.
Bhakta S
Mycobacteria Research Laboratory, Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck, University of London, London, UK.
Gray AI
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.
Seidel V
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK. Electronic address: veronique.seidel@strath.ac.uk.

MeSH

Antitubercular AgentsArctiumEthnopharmacologyMagnetic Resonance SpectroscopyMass SpectrometryMedicine, TraditionalMicrobial Sensitivity TestsMycobacterium tuberculosisPlant ExtractsTussilago

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24955560

Citation

Zhao, Jinlian, et al. "Antitubercular Activity of Arctium Lappa and Tussilago Farfara Extracts and Constituents." Journal of Ethnopharmacology, vol. 155, no. 1, 2014, pp. 796-800.
Zhao J, Evangelopoulos D, Bhakta S, et al. Antitubercular activity of Arctium lappa and Tussilago farfara extracts and constituents. J Ethnopharmacol. 2014;155(1):796-800.
Zhao, J., Evangelopoulos, D., Bhakta, S., Gray, A. I., & Seidel, V. (2014). Antitubercular activity of Arctium lappa and Tussilago farfara extracts and constituents. Journal of Ethnopharmacology, 155(1), 796-800. https://doi.org/10.1016/j.jep.2014.06.034
Zhao J, et al. Antitubercular Activity of Arctium Lappa and Tussilago Farfara Extracts and Constituents. J Ethnopharmacol. 2014 Aug 8;155(1):796-800. PubMed PMID: 24955560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antitubercular activity of Arctium lappa and Tussilago farfara extracts and constituents. AU - Zhao,Jinlian, AU - Evangelopoulos,Dimitrios, AU - Bhakta,Sanjib, AU - Gray,Alexander I, AU - Seidel,Véronique, Y1 - 2014/06/20/ PY - 2014/02/13/received PY - 2014/05/28/revised PY - 2014/06/14/accepted PY - 2014/6/24/entrez PY - 2014/6/24/pubmed PY - 2015/4/8/medline KW - Antitubercular activity KW - Arctium lappa KW - Mycobacterium tuberculosis KW - Tussilago farfara SP - 796 EP - 800 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 155 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Arctium lappa and Tussilago farfara (Asteraceae) are two plant species used traditionally as antitubercular remedies. The aim of this study was (i) to screen Arctium lappa and Tussilago farfara extracts for activity against Mycobacterium tuberculosis and (ii) to isolate and identify the compound(s) responsible for this reputed anti-TB effect. MATERIALS AND METHODS: The activity of extracts and isolated compounds was determined against Mycobacterium tuberculosis H37Rv using a high throughput spot culture growth inhibition (HT-SPOTi) assay. RESULTS: The n-hexane extracts of both plants, the ethyl acetate extract of Tussilago farfara and the dichloromethane phase derived from the methanol extract of Arctium lappa displayed antitubercular activity (MIC 62.5 μg/mL). Further chemical investigation of Arctium lappa led to the isolation of n-nonacosane (1), taraxasterol acetate (2), taraxasterol (3), a (1:1) mixture of β sitosterol/stigmasterol (4), isololiolide (5), melitensin (6), trans-caffeic acid (7), kaempferol (8), quercetin (9), kaempferol-3-O-glucoside (10). Compounds isolated from Tussilago farfara were identified as a (1:1) mixture of β sitosterol/stigmasterol (4), trans-caffeic acid (7), kaempferol (8), quercetin (9), kaempferol-3-O-glucoside (10), loliolide (11), a (4:1) mixture of p-coumaric acid/4-hydroxybenzoic acid (12), p-coumaric acid (13). All compounds were identified following analyses of their physicochemical and spectroscopic data (MS, (1)H and (13)C-NMR) and by comparison with published data. This is the first report of the isolation of n-nonacosane (1), isololiolide (5), melitensin (6) and kaempferol-3-O-glucoside (10) from Arctium lappa, and of loliolide (11) from Tussilago farfara. Amongst the isolated compounds, the best activity was observed for p-coumaric acid (13) (MIC 31.3 μg/mL or 190.9 μM) alone and in mixture with 4-hydroxybenzoic acid (12) (MIC 62.5 μg/mL). CONCLUSIONS: The above results provide for the first time some scientific evidence to support, to some extent, the ethno-medicinal use of Arctium lappa and Tussilago farfara as traditional antitubercular remedies. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/24955560/Antitubercular_activity_of_Arctium_lappa_and_Tussilago_farfara_extracts_and_constituents_ DB - PRIME DP - Unbound Medicine ER -