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Transcriptome profiling reveals roles of meristem regulators and polarity genes during fruit trichome development in cucumber (Cucumis sativus L.).
J Exp Bot. 2014 Sep; 65(17):4943-58.JE

Abstract

Trichomes are epidermal hair-like structures that function in plant defence against biotic and abiotic stresses. Extensive studies have been performed on foliar trichomes development in Arabidopsis and tomato, but the molecular mechanism of fruit trichome formation remains elusive. Cucumber fruit is covered with trichomes (spines) that directly affect the appearance and quality of cucumber products. Here, we characterized the fruit spine development in wild-type (WT) cucumber and a spontaneous mutant, tiny branched hair (tbh). Our data showed that the cucumber trichome was multicellular and non-glandular, with malformed organelles and no endoreduplication. Fruit spine development was generally homogenous and marked by a rapid base expansion stage. Trichomes in the tbh mutant were tiny and branched, with increased density and aberrant cell shape. Transcriptome profiling indicated that meristem-related genes were highly enriched in the upregulated genes in the tbh versus the WT, as well as in WT spines after versus before base expansion, and that polarity regulators were greatly induced during spine base expansion. Quantitative reverse transcription PCR and in situ hybridization confirmed the differential expression of CUP-SHAPED COTYLEDON3 (CUC3) and SHOOT MERISTEMLESS (STM) during spine development. Therefore, cucumber trichomes are morphologically different from those of Arabidopsis and tomato, and their development may be regulated by a distinct pathway involving meristem genes and polarity regulators.

Authors+Show Affiliations

Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, PR China.Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, PR China.Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, PR China.Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, PR China.Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, PR China.Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, PR China.Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, PR China.Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, PR China zhxiaolan@cau.edu.cn renyi.liu@ucr.edu renhuazhong@cau.edu.cn.Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA zhxiaolan@cau.edu.cn renyi.liu@ucr.edu renhuazhong@cau.edu.cn.Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, PR China zhxiaolan@cau.edu.cn renyi.liu@ucr.edu renhuazhong@cau.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24962999

Citation

Chen, Chunhua, et al. "Transcriptome Profiling Reveals Roles of Meristem Regulators and Polarity Genes During Fruit Trichome Development in Cucumber (Cucumis Sativus L.)." Journal of Experimental Botany, vol. 65, no. 17, 2014, pp. 4943-58.
Chen C, Liu M, Jiang L, et al. Transcriptome profiling reveals roles of meristem regulators and polarity genes during fruit trichome development in cucumber (Cucumis sativus L.). J Exp Bot. 2014;65(17):4943-58.
Chen, C., Liu, M., Jiang, L., Liu, X., Zhao, J., Yan, S., Yang, S., Ren, H., Liu, R., & Zhang, X. (2014). Transcriptome profiling reveals roles of meristem regulators and polarity genes during fruit trichome development in cucumber (Cucumis sativus L.). Journal of Experimental Botany, 65(17), 4943-58. https://doi.org/10.1093/jxb/eru258
Chen C, et al. Transcriptome Profiling Reveals Roles of Meristem Regulators and Polarity Genes During Fruit Trichome Development in Cucumber (Cucumis Sativus L.). J Exp Bot. 2014;65(17):4943-58. PubMed PMID: 24962999.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptome profiling reveals roles of meristem regulators and polarity genes during fruit trichome development in cucumber (Cucumis sativus L.). AU - Chen,Chunhua, AU - Liu,Meiling, AU - Jiang,Li, AU - Liu,Xiaofeng, AU - Zhao,Jianyu, AU - Yan,Shuangshuang, AU - Yang,Sen, AU - Ren,Huazhong, AU - Liu,Renyi, AU - Zhang,Xiaolan, Y1 - 2014/06/24/ PY - 2014/6/26/entrez PY - 2014/6/26/pubmed PY - 2015/5/29/medline KW - Cucumber KW - fruit spine KW - meristem regulator KW - polarity transcriptome KW - trichome. SP - 4943 EP - 58 JF - Journal of experimental botany JO - J Exp Bot VL - 65 IS - 17 N2 - Trichomes are epidermal hair-like structures that function in plant defence against biotic and abiotic stresses. Extensive studies have been performed on foliar trichomes development in Arabidopsis and tomato, but the molecular mechanism of fruit trichome formation remains elusive. Cucumber fruit is covered with trichomes (spines) that directly affect the appearance and quality of cucumber products. Here, we characterized the fruit spine development in wild-type (WT) cucumber and a spontaneous mutant, tiny branched hair (tbh). Our data showed that the cucumber trichome was multicellular and non-glandular, with malformed organelles and no endoreduplication. Fruit spine development was generally homogenous and marked by a rapid base expansion stage. Trichomes in the tbh mutant were tiny and branched, with increased density and aberrant cell shape. Transcriptome profiling indicated that meristem-related genes were highly enriched in the upregulated genes in the tbh versus the WT, as well as in WT spines after versus before base expansion, and that polarity regulators were greatly induced during spine base expansion. Quantitative reverse transcription PCR and in situ hybridization confirmed the differential expression of CUP-SHAPED COTYLEDON3 (CUC3) and SHOOT MERISTEMLESS (STM) during spine development. Therefore, cucumber trichomes are morphologically different from those of Arabidopsis and tomato, and their development may be regulated by a distinct pathway involving meristem genes and polarity regulators. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/24962999/Transcriptome_profiling_reveals_roles_of_meristem_regulators_and_polarity_genes_during_fruit_trichome_development_in_cucumber__Cucumis_sativus_L___ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/eru258 DB - PRIME DP - Unbound Medicine ER -