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CB2 receptor activation ameliorates the proinflammatory activity in acute lung injury induced by paraquat.
Biomed Res Int. 2014; 2014:971750.BR

Abstract

Paraquat, a widely used herbicide, is well known to exhibit oxidative stress and lung injury. In the present study, we investigated the possible underlying mechanisms of cannabinoid receptor-2 (CB2) activation to ameliorate the proinflammatory activity induced by PQ in rats. JWH133, a CB2 agonist, was administered by intraperitoneal injection 1 h prior to PQ exposure. After PQ exposure for 4, 8, 24, and 72 h, the bronchoalveolar lavage fluid was collected to determine levels of TNF-α and IL-1β, and the arterial blood samples were collected for detection of PaO2 level. At 72 h after PQ exposure, lung tissues were collected to determine the lung wet-to-dry weight ratios, myeloperoxidase activity, lung histopathology, the protein expression level of CB2, MAPKs (ERK1/2, p38MAPK, and JNK1/2), and NF-κBp65. After rats were pretreated with JWH133, PQ-induced lung edema and lung histopathological changes were significantly attenuated. PQ-induced TNF-α and IL-1β secretion in BALF, increases of PaO2 in arterial blood, and MPO levels in the lung tissue were significantly reduced. JWH133 could efficiently activate CB2, while inhibiting MAPKs and NF-κB activation. The results suggested that activating CB2 receptor exerted protective activity against PQ-induced ALI, and it potentially contributed to the suppression of the activation of MAPKs and NF-κB pathways.

Authors+Show Affiliations

Department of Emergency Medicine, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, Liaoning 110004, China.Department of Emergency Medicine, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, Liaoning 110004, China.Department of Emergency Medicine, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, Liaoning 110004, China.Department of Emergency Medicine, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, Liaoning 110004, China.Department of Emergency Medicine, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, Liaoning 110004, China.Department of Emergency Medicine, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, Liaoning 110004, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24963491

Citation

Liu, Zhenning, et al. "CB2 Receptor Activation Ameliorates the Proinflammatory Activity in Acute Lung Injury Induced By Paraquat." BioMed Research International, vol. 2014, 2014, p. 971750.
Liu Z, Wang Y, Zhao H, et al. CB2 receptor activation ameliorates the proinflammatory activity in acute lung injury induced by paraquat. Biomed Res Int. 2014;2014:971750.
Liu, Z., Wang, Y., Zhao, H., Zheng, Q., Xiao, L., & Zhao, M. (2014). CB2 receptor activation ameliorates the proinflammatory activity in acute lung injury induced by paraquat. BioMed Research International, 2014, 971750. https://doi.org/10.1155/2014/971750
Liu Z, et al. CB2 Receptor Activation Ameliorates the Proinflammatory Activity in Acute Lung Injury Induced By Paraquat. Biomed Res Int. 2014;2014:971750. PubMed PMID: 24963491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CB2 receptor activation ameliorates the proinflammatory activity in acute lung injury induced by paraquat. AU - Liu,Zhenning, AU - Wang,Yu, AU - Zhao,Hongyu, AU - Zheng,Qiang, AU - Xiao,Li, AU - Zhao,Min, Y1 - 2014/05/22/ PY - 2014/03/08/received PY - 2014/05/08/accepted PY - 2014/6/26/entrez PY - 2014/6/26/pubmed PY - 2015/2/13/medline SP - 971750 EP - 971750 JF - BioMed research international JO - Biomed Res Int VL - 2014 N2 - Paraquat, a widely used herbicide, is well known to exhibit oxidative stress and lung injury. In the present study, we investigated the possible underlying mechanisms of cannabinoid receptor-2 (CB2) activation to ameliorate the proinflammatory activity induced by PQ in rats. JWH133, a CB2 agonist, was administered by intraperitoneal injection 1 h prior to PQ exposure. After PQ exposure for 4, 8, 24, and 72 h, the bronchoalveolar lavage fluid was collected to determine levels of TNF-α and IL-1β, and the arterial blood samples were collected for detection of PaO2 level. At 72 h after PQ exposure, lung tissues were collected to determine the lung wet-to-dry weight ratios, myeloperoxidase activity, lung histopathology, the protein expression level of CB2, MAPKs (ERK1/2, p38MAPK, and JNK1/2), and NF-κBp65. After rats were pretreated with JWH133, PQ-induced lung edema and lung histopathological changes were significantly attenuated. PQ-induced TNF-α and IL-1β secretion in BALF, increases of PaO2 in arterial blood, and MPO levels in the lung tissue were significantly reduced. JWH133 could efficiently activate CB2, while inhibiting MAPKs and NF-κB activation. The results suggested that activating CB2 receptor exerted protective activity against PQ-induced ALI, and it potentially contributed to the suppression of the activation of MAPKs and NF-κB pathways. SN - 2314-6141 UR - https://www.unboundmedicine.com/medline/citation/24963491/CB2_receptor_activation_ameliorates_the_proinflammatory_activity_in_acute_lung_injury_induced_by_paraquat_ L2 - https://doi.org/10.1155/2014/971750 DB - PRIME DP - Unbound Medicine ER -