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Bromelain: an overview of industrial application and purification strategies.
Appl Microbiol Biotechnol 2014; 98(17):7283-97AM

Abstract

This review highlights the use of bromelain in various applications with up-to-date literature on the purification of bromelain from pineapple fruit and waste such as peel, core, crown, and leaves. Bromelain, a cysteine protease, has been exploited commercially in many applications in the food, beverage, tenderization, cosmetic, pharmaceutical, and textile industries. Researchers worldwide have been directing their interest to purification strategies by applying conventional and modern approaches, such as manipulating the pH, affinity, hydrophobicity, and temperature conditions in accord with the unique properties of bromelain. The amount of downstream processing will depend on its intended application in industries. The breakthrough of recombinant DNA technology has facilitated the large-scale production and purification of recombinant bromelain for novel applications in the future.

Authors+Show Affiliations

Bioprocess and Molecular Engineering Research Unit, Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

24965557

Citation

Arshad, Zatul Iffah Mohd, et al. "Bromelain: an Overview of Industrial Application and Purification Strategies." Applied Microbiology and Biotechnology, vol. 98, no. 17, 2014, pp. 7283-97.
Arshad ZI, Amid A, Yusof F, et al. Bromelain: an overview of industrial application and purification strategies. Appl Microbiol Biotechnol. 2014;98(17):7283-97.
Arshad, Z. I., Amid, A., Yusof, F., Jaswir, I., Ahmad, K., & Loke, S. P. (2014). Bromelain: an overview of industrial application and purification strategies. Applied Microbiology and Biotechnology, 98(17), pp. 7283-97. doi:10.1007/s00253-014-5889-y.
Arshad ZI, et al. Bromelain: an Overview of Industrial Application and Purification Strategies. Appl Microbiol Biotechnol. 2014;98(17):7283-97. PubMed PMID: 24965557.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bromelain: an overview of industrial application and purification strategies. AU - Arshad,Zatul Iffah Mohd, AU - Amid,Azura, AU - Yusof,Faridah, AU - Jaswir,Irwandi, AU - Ahmad,Kausar, AU - Loke,Show Pau, Y1 - 2014/06/26/ PY - 2014/03/19/received PY - 2014/06/10/accepted PY - 2014/06/08/revised PY - 2014/6/27/entrez PY - 2014/6/27/pubmed PY - 2015/5/13/medline SP - 7283 EP - 97 JF - Applied microbiology and biotechnology JO - Appl. Microbiol. Biotechnol. VL - 98 IS - 17 N2 - This review highlights the use of bromelain in various applications with up-to-date literature on the purification of bromelain from pineapple fruit and waste such as peel, core, crown, and leaves. Bromelain, a cysteine protease, has been exploited commercially in many applications in the food, beverage, tenderization, cosmetic, pharmaceutical, and textile industries. Researchers worldwide have been directing their interest to purification strategies by applying conventional and modern approaches, such as manipulating the pH, affinity, hydrophobicity, and temperature conditions in accord with the unique properties of bromelain. The amount of downstream processing will depend on its intended application in industries. The breakthrough of recombinant DNA technology has facilitated the large-scale production and purification of recombinant bromelain for novel applications in the future. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/24965557/Bromelain:_an_overview_of_industrial_application_and_purification_strategies_ L2 - https://dx.doi.org/10.1007/s00253-014-5889-y DB - PRIME DP - Unbound Medicine ER -