Tags

Type your tag names separated by a space and hit enter

Subtype-specific mechanisms for functional interaction between α6β4* nicotinic acetylcholine receptors and P2X receptors.
Mol Pharmacol 2014; 86(3):263-74MP

Abstract

P2X receptors and nicotinic acetylcholine receptors (nAChRs) display functional and physical interactions in many cell types and heterologous expression systems, but interactions between α6β4-containing (α6β4*) nAChRs and P2X2 receptors and/or P2X3 receptors have not been fully characterized. We measured several types of crosstalk in oocytes coexpressing α6β4 nAChRs and P2X2, P2X3, or P2X2/3 receptors. A novel form of crosstalk occurs between α6β4 nAChRs and P2X2 receptors. P2X2 receptors were forced into a prolonged desensitized state upon activation by ATP through a mechanism that does not depend on the intracellular C terminus of the P2X2 receptors. Coexpression of α6β4 nAChRs with P2X3 receptors shifts the ATP dose-response relation to the right, even in the absence of acetylcholine (ACh). Moreover, currents become nonadditive when ACh and ATP are coapplied, as previously reported for other Cys-loop receptors interacting with P2X receptors, and this crosstalk is dependent on the presence of the P2X3 C-terminal domain. P2X2 receptors also functionally interact with α6β4β3 but through a different mechanism from α6β4. The interaction with P2X3 receptors is less pronounced for the α6β4β3 nAChR than the α6β4 nAChR. We also measured a functional interaction between the α6β4 nAChRs and the heteromeric P2X2/3 receptor. Experiments with the nAChR channel blocker mecamylamine on P2X2-α6β4 oocytes point to the loss of P2X2 channel activity during the crosstalk, whereas the ion channel pores of the P2X receptors were fully functional and unaltered by the receptor interaction for P2X2-α6β4β3, P2X2/3-α6β4, and P2X2/3-α6β4β3. These results may be relevant to dorsal root ganglion cells and to other neurons that coexpress these receptor subunits.

Authors+Show Affiliations

Divisions of Chemistry and Chemical Engineering (W.L., D.A.D.) and Biology and Biological Engineering (H.A.L.), California Institute of Technology, Pasadena, California.Divisions of Chemistry and Chemical Engineering (W.L., D.A.D.) and Biology and Biological Engineering (H.A.L.), California Institute of Technology, Pasadena, California.Divisions of Chemistry and Chemical Engineering (W.L., D.A.D.) and Biology and Biological Engineering (H.A.L.), California Institute of Technology, Pasadena, California lester@caltech.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24966348

Citation

Limapichat, Walrati, et al. "Subtype-specific Mechanisms for Functional Interaction Between Α6β4* Nicotinic Acetylcholine Receptors and P2X Receptors." Molecular Pharmacology, vol. 86, no. 3, 2014, pp. 263-74.
Limapichat W, Dougherty DA, Lester HA. Subtype-specific mechanisms for functional interaction between α6β4* nicotinic acetylcholine receptors and P2X receptors. Mol Pharmacol. 2014;86(3):263-74.
Limapichat, W., Dougherty, D. A., & Lester, H. A. (2014). Subtype-specific mechanisms for functional interaction between α6β4* nicotinic acetylcholine receptors and P2X receptors. Molecular Pharmacology, 86(3), pp. 263-74. doi:10.1124/mol.114.093179.
Limapichat W, Dougherty DA, Lester HA. Subtype-specific Mechanisms for Functional Interaction Between Α6β4* Nicotinic Acetylcholine Receptors and P2X Receptors. Mol Pharmacol. 2014;86(3):263-74. PubMed PMID: 24966348.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Subtype-specific mechanisms for functional interaction between α6β4* nicotinic acetylcholine receptors and P2X receptors. AU - Limapichat,Walrati, AU - Dougherty,Dennis A, AU - Lester,Henry A, Y1 - 2014/06/25/ PY - 2014/6/27/entrez PY - 2014/6/27/pubmed PY - 2014/9/30/medline SP - 263 EP - 74 JF - Molecular pharmacology JO - Mol. Pharmacol. VL - 86 IS - 3 N2 - P2X receptors and nicotinic acetylcholine receptors (nAChRs) display functional and physical interactions in many cell types and heterologous expression systems, but interactions between α6β4-containing (α6β4*) nAChRs and P2X2 receptors and/or P2X3 receptors have not been fully characterized. We measured several types of crosstalk in oocytes coexpressing α6β4 nAChRs and P2X2, P2X3, or P2X2/3 receptors. A novel form of crosstalk occurs between α6β4 nAChRs and P2X2 receptors. P2X2 receptors were forced into a prolonged desensitized state upon activation by ATP through a mechanism that does not depend on the intracellular C terminus of the P2X2 receptors. Coexpression of α6β4 nAChRs with P2X3 receptors shifts the ATP dose-response relation to the right, even in the absence of acetylcholine (ACh). Moreover, currents become nonadditive when ACh and ATP are coapplied, as previously reported for other Cys-loop receptors interacting with P2X receptors, and this crosstalk is dependent on the presence of the P2X3 C-terminal domain. P2X2 receptors also functionally interact with α6β4β3 but through a different mechanism from α6β4. The interaction with P2X3 receptors is less pronounced for the α6β4β3 nAChR than the α6β4 nAChR. We also measured a functional interaction between the α6β4 nAChRs and the heteromeric P2X2/3 receptor. Experiments with the nAChR channel blocker mecamylamine on P2X2-α6β4 oocytes point to the loss of P2X2 channel activity during the crosstalk, whereas the ion channel pores of the P2X receptors were fully functional and unaltered by the receptor interaction for P2X2-α6β4β3, P2X2/3-α6β4, and P2X2/3-α6β4β3. These results may be relevant to dorsal root ganglion cells and to other neurons that coexpress these receptor subunits. SN - 1521-0111 UR - https://www.unboundmedicine.com/medline/citation/24966348/Subtype_specific_mechanisms_for_functional_interaction_between_α6β4__nicotinic_acetylcholine_receptors_and_P2X_receptors_ L2 - http://molpharm.aspetjournals.org/cgi/pmidlookup?view=long&pmid=24966348 DB - PRIME DP - Unbound Medicine ER -