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Protective effect of bone marrow mesenchymal stem cells in intestinal barrier permeability after heterotopic intestinal transplantation.
World J Gastroenterol. 2014 Jun 21; 20(23):7442-51.WJ

Abstract

AIM

To explore the protective effect of bone marrow mesenchymal stem cells (BM MSCs) in the small intestinal mucosal barrier following heterotopic intestinal transplantation (HIT) in a rat model.

METHODS

BM MSCs were isolated from male Lewis rats by density gradient centrifugation, cultured, and analyzed by flow cytometry. The HIT models were divided into a non-rejection group, saline-treated rejection group (via penile vein), and BM MSC-treated group (via penile vein). Intestinal mucosal barrier injury was estimated by diamine oxidase (DAO) and D-lactic acid (D-LA) expression levels. Tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ), interleukin-10 (IL-10), and transforming growth factor-β (TGF-β) were detected by enzyme-linked immunosorbent assay. Ultrastructural change of tight junctions (TJs) was observed under transmission electron microscope. Expression levels of the TJ proteins occludin and zona occludens (ZO)-1, affected by the inflammatory factors, were measured using real-time polymerase chain reaction and Western blotting.

RESULTS

The pathological score at each time point after surgery indicated significantly less serious injury in the BM MSCs-treated group than in the rejection group (P < 0.05). In the former, graft levels of DAO and D-LA were reduced, and TNF-α and INF-γ production was inhibited (at day 7: 10.6473 ± 0.0710 vs 17.2128 ± 0.4991, P < 0.05; 545.1506 ± 31.9416 vs 810.2637 ± 25.1175, P < 0.05). IL-10 and TGF-β production was increased greatly (at day 7: 125.7773 ± 4.7719 vs 80.3756 ± 2.5866, P < 0.05; 234.5273 ± 9.3980 vs 545.1506 ± 31.9416, P < 0.05). There was increased expression of occludin and ZO-1 protein (at day 7: 0.2674 ± 0.0128 vs 0.1352 ± 0.0142, P < 0.05; at day 5: 0.7189 ± 0.0289 vs 0.4556 ± 0.0242, P < 0.05) and mRNA (at day 7: 0.3860 ± 0.0254 vs 0.1673 ± 0.0369, P < 0.05; at day 5: 0.5727 ± 0.0419 vs 0.3598 ± 0.0242, P < 0.05).

CONCLUSION

BM MSCs can improve intestinal barrier permeability, repair TJs, and increase occludin and ZO-1 protein expression. With altered cytokine levels, they can protect the intestinal mucosa after transplantation.

Authors+Show Affiliations

Wen Zhang, Zhong-Yang Shen, Hong-Li Song, Yang Yang, Ben-Juan Wu, Nan-Nan Fu, Department of Organ Transplantation, Tianjin First Central Hospital, Tianjin 300192, China.Wen Zhang, Zhong-Yang Shen, Hong-Li Song, Yang Yang, Ben-Juan Wu, Nan-Nan Fu, Department of Organ Transplantation, Tianjin First Central Hospital, Tianjin 300192, China.Wen Zhang, Zhong-Yang Shen, Hong-Li Song, Yang Yang, Ben-Juan Wu, Nan-Nan Fu, Department of Organ Transplantation, Tianjin First Central Hospital, Tianjin 300192, China.Wen Zhang, Zhong-Yang Shen, Hong-Li Song, Yang Yang, Ben-Juan Wu, Nan-Nan Fu, Department of Organ Transplantation, Tianjin First Central Hospital, Tianjin 300192, China.Wen Zhang, Zhong-Yang Shen, Hong-Li Song, Yang Yang, Ben-Juan Wu, Nan-Nan Fu, Department of Organ Transplantation, Tianjin First Central Hospital, Tianjin 300192, China.Wen Zhang, Zhong-Yang Shen, Hong-Li Song, Yang Yang, Ben-Juan Wu, Nan-Nan Fu, Department of Organ Transplantation, Tianjin First Central Hospital, Tianjin 300192, China.Wen Zhang, Zhong-Yang Shen, Hong-Li Song, Yang Yang, Ben-Juan Wu, Nan-Nan Fu, Department of Organ Transplantation, Tianjin First Central Hospital, Tianjin 300192, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24966614

Citation

Zhang, Wen, et al. "Protective Effect of Bone Marrow Mesenchymal Stem Cells in Intestinal Barrier Permeability After Heterotopic Intestinal Transplantation." World Journal of Gastroenterology, vol. 20, no. 23, 2014, pp. 7442-51.
Zhang W, Shen ZY, Song HL, et al. Protective effect of bone marrow mesenchymal stem cells in intestinal barrier permeability after heterotopic intestinal transplantation. World J Gastroenterol. 2014;20(23):7442-51.
Zhang, W., Shen, Z. Y., Song, H. L., Yang, Y., Wu, B. J., Fu, N. N., & Liu, T. (2014). Protective effect of bone marrow mesenchymal stem cells in intestinal barrier permeability after heterotopic intestinal transplantation. World Journal of Gastroenterology, 20(23), 7442-51. https://doi.org/10.3748/wjg.v20.i23.7442
Zhang W, et al. Protective Effect of Bone Marrow Mesenchymal Stem Cells in Intestinal Barrier Permeability After Heterotopic Intestinal Transplantation. World J Gastroenterol. 2014 Jun 21;20(23):7442-51. PubMed PMID: 24966614.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of bone marrow mesenchymal stem cells in intestinal barrier permeability after heterotopic intestinal transplantation. AU - Zhang,Wen, AU - Shen,Zhong-Yang, AU - Song,Hong-Li, AU - Yang,Yang, AU - Wu,Ben-Juan, AU - Fu,Nan-Nan, AU - Liu,Tao, PY - 2014/01/09/received PY - 2014/03/11/revised PY - 2014/04/21/accepted PY - 2014/6/27/entrez PY - 2014/6/27/pubmed PY - 2015/4/14/medline KW - Bone marrow mesenchymal stem cells KW - Intestinal mucosal barrier KW - Occludin KW - Small intestinal transplantation KW - Zona occludens-1 SP - 7442 EP - 51 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 20 IS - 23 N2 - AIM: To explore the protective effect of bone marrow mesenchymal stem cells (BM MSCs) in the small intestinal mucosal barrier following heterotopic intestinal transplantation (HIT) in a rat model. METHODS: BM MSCs were isolated from male Lewis rats by density gradient centrifugation, cultured, and analyzed by flow cytometry. The HIT models were divided into a non-rejection group, saline-treated rejection group (via penile vein), and BM MSC-treated group (via penile vein). Intestinal mucosal barrier injury was estimated by diamine oxidase (DAO) and D-lactic acid (D-LA) expression levels. Tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ), interleukin-10 (IL-10), and transforming growth factor-β (TGF-β) were detected by enzyme-linked immunosorbent assay. Ultrastructural change of tight junctions (TJs) was observed under transmission electron microscope. Expression levels of the TJ proteins occludin and zona occludens (ZO)-1, affected by the inflammatory factors, were measured using real-time polymerase chain reaction and Western blotting. RESULTS: The pathological score at each time point after surgery indicated significantly less serious injury in the BM MSCs-treated group than in the rejection group (P < 0.05). In the former, graft levels of DAO and D-LA were reduced, and TNF-α and INF-γ production was inhibited (at day 7: 10.6473 ± 0.0710 vs 17.2128 ± 0.4991, P < 0.05; 545.1506 ± 31.9416 vs 810.2637 ± 25.1175, P < 0.05). IL-10 and TGF-β production was increased greatly (at day 7: 125.7773 ± 4.7719 vs 80.3756 ± 2.5866, P < 0.05; 234.5273 ± 9.3980 vs 545.1506 ± 31.9416, P < 0.05). There was increased expression of occludin and ZO-1 protein (at day 7: 0.2674 ± 0.0128 vs 0.1352 ± 0.0142, P < 0.05; at day 5: 0.7189 ± 0.0289 vs 0.4556 ± 0.0242, P < 0.05) and mRNA (at day 7: 0.3860 ± 0.0254 vs 0.1673 ± 0.0369, P < 0.05; at day 5: 0.5727 ± 0.0419 vs 0.3598 ± 0.0242, P < 0.05). CONCLUSION: BM MSCs can improve intestinal barrier permeability, repair TJs, and increase occludin and ZO-1 protein expression. With altered cytokine levels, they can protect the intestinal mucosa after transplantation. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/24966614/Protective_effect_of_bone_marrow_mesenchymal_stem_cells_in_intestinal_barrier_permeability_after_heterotopic_intestinal_transplantation_ L2 - http://www.wjgnet.com/1007-9327/full/v20/i23/7442.htm DB - PRIME DP - Unbound Medicine ER -