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Ultrasensitive sandwich-type electrochemical immunosensor based on a novel signal amplification strategy using highly loaded toluidine blue/gold nanoparticles decorated KIT-6/carboxymethyl chitosan/ionic liquids as signal labels.
Biosens Bioelectron. 2014 Nov 15; 61:618-24.BB

Abstract

An ultrasensitive sandwich-type electrochemical immunosensor for the quantitative detection of carcinoembryonic antigen (CEA) was designed based on a novel signal amplification strategy. Gold nanoparticles decorated mesoporous silica KIT-6 (Au@KIT-6) with large specific surface area and good adsorption properties was used as a label matrix to immobilize both the secondary antibodies (Ab2) and the electron transfer mediator toluidine blue (TB). Ab2 was loaded on KIT-6 due to the presence of gold nanoparticles (Au NPs) and TB was loaded on KIT-6 by physical adsorption. Ionic liquids (ILs) doped carboxymethyl chitosan (CMC) was used to prevent the leak of TB and facilitate the electron transfer. For the immobilization of primary antibodies (Ab1), gold nanoparticles decorated 3-aminopropyltriethoxysilane functionalized graphene sheets (Au@APTES-GS) were used as transducing materials to modify glassy carbon electrodes (GCE). High sensitivity was achieved for the designed immunosensor based on this novel signal amplification strategy. Under optimal conditions, the immunosensor exhibited an extremely low detection limit of 3.3 fg/mL and wide liner range from 10(-5) ng/mL to 10(2) ng/mL for CEA. Moreover, it exhibited good selectivity, acceptable reproducibility and stability, indicating potential application promising in clinical monitoring of tumor biomarkers.

Authors+Show Affiliations

Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: sdjndxwq@163.com.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24967751

Citation

Wang, Yulan, et al. "Ultrasensitive Sandwich-type Electrochemical Immunosensor Based On a Novel Signal Amplification Strategy Using Highly Loaded Toluidine Blue/gold Nanoparticles Decorated KIT-6/carboxymethyl Chitosan/ionic Liquids as Signal Labels." Biosensors & Bioelectronics, vol. 61, 2014, pp. 618-24.
Wang Y, Li X, Cao W, et al. Ultrasensitive sandwich-type electrochemical immunosensor based on a novel signal amplification strategy using highly loaded toluidine blue/gold nanoparticles decorated KIT-6/carboxymethyl chitosan/ionic liquids as signal labels. Biosens Bioelectron. 2014;61:618-24.
Wang, Y., Li, X., Cao, W., Li, Y., Li, H., Du, B., & Wei, Q. (2014). Ultrasensitive sandwich-type electrochemical immunosensor based on a novel signal amplification strategy using highly loaded toluidine blue/gold nanoparticles decorated KIT-6/carboxymethyl chitosan/ionic liquids as signal labels. Biosensors & Bioelectronics, 61, 618-24. https://doi.org/10.1016/j.bios.2014.05.059
Wang Y, et al. Ultrasensitive Sandwich-type Electrochemical Immunosensor Based On a Novel Signal Amplification Strategy Using Highly Loaded Toluidine Blue/gold Nanoparticles Decorated KIT-6/carboxymethyl Chitosan/ionic Liquids as Signal Labels. Biosens Bioelectron. 2014 Nov 15;61:618-24. PubMed PMID: 24967751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrasensitive sandwich-type electrochemical immunosensor based on a novel signal amplification strategy using highly loaded toluidine blue/gold nanoparticles decorated KIT-6/carboxymethyl chitosan/ionic liquids as signal labels. AU - Wang,Yulan, AU - Li,Xiaojian, AU - Cao,Wei, AU - Li,Yueyun, AU - Li,He, AU - Du,Bin, AU - Wei,Qin, Y1 - 2014/06/06/ PY - 2014/02/26/received PY - 2014/05/26/revised PY - 2014/05/29/accepted PY - 2014/6/27/entrez PY - 2014/6/27/pubmed PY - 2015/2/24/medline KW - Carboxymethyl chitosan KW - Carcinoembryonic antigen KW - Ionic liquids KW - KIT-6 KW - Sandwich-type electrochemical immunosensor SP - 618 EP - 24 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 61 N2 - An ultrasensitive sandwich-type electrochemical immunosensor for the quantitative detection of carcinoembryonic antigen (CEA) was designed based on a novel signal amplification strategy. Gold nanoparticles decorated mesoporous silica KIT-6 (Au@KIT-6) with large specific surface area and good adsorption properties was used as a label matrix to immobilize both the secondary antibodies (Ab2) and the electron transfer mediator toluidine blue (TB). Ab2 was loaded on KIT-6 due to the presence of gold nanoparticles (Au NPs) and TB was loaded on KIT-6 by physical adsorption. Ionic liquids (ILs) doped carboxymethyl chitosan (CMC) was used to prevent the leak of TB and facilitate the electron transfer. For the immobilization of primary antibodies (Ab1), gold nanoparticles decorated 3-aminopropyltriethoxysilane functionalized graphene sheets (Au@APTES-GS) were used as transducing materials to modify glassy carbon electrodes (GCE). High sensitivity was achieved for the designed immunosensor based on this novel signal amplification strategy. Under optimal conditions, the immunosensor exhibited an extremely low detection limit of 3.3 fg/mL and wide liner range from 10(-5) ng/mL to 10(2) ng/mL for CEA. Moreover, it exhibited good selectivity, acceptable reproducibility and stability, indicating potential application promising in clinical monitoring of tumor biomarkers. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/24967751/Ultrasensitive_sandwich_type_electrochemical_immunosensor_based_on_a_novel_signal_amplification_strategy_using_highly_loaded_toluidine_blue/gold_nanoparticles_decorated_KIT_6/carboxymethyl_chitosan/ionic_liquids_as_signal_labels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(14)00401-1 DB - PRIME DP - Unbound Medicine ER -