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Antioxidant activity profiling by spectrophotometric methods of aqueous methanolic extracts of Helichrysum stoechas subsp. rupestre and Phagnalon saxatile subsp. saxatile.
Chin J Nat Med. 2014 Jun; 12(6):415-22.CJ

Abstract

AIM

The aqueous methanolic extracts of two plants from Algeria, Helichrysum stoechas subsp. rupestre and Phagnalon saxatile subsp. saxatile, were investigated for their antioxidant activity.

METHOD

Total phenolics, flavonoids, and tannins were determined by spectrophotometric techniques. In vitro antioxidant and radical scavenging profiling was determined by spectrophotometric methods, through: Total antioxidant capacity, and radical scavenging effects by the DPPH and ABTS methods, reducing and chelating power, and blanching inhibition of the β-carotene.

RESULTS

All of the extracts showed interesting antioxidant and radical scavenging activity. The highest contents in phenolics, tannins, and the highest total antioxidant capacity as gallic acid equivalents of 97.5 ± 0.33 mg GAE/g DW was obtained for the flowers of H. stoechas subsp. rupestre extract in the phosphomolybdenum assay. An extract of the leafy stems of P. saxatile subsp. saxatile revealed the highest content of flavonoids, and the highest antioxidant activity by the radical scavenging and β-carotene assays when compared with standards. The best activity was by the scavenging radical DPPH with an IC50 value of 5.65 ± 0.10 μg·mL(-1).

CONCLUSION

The studied medicinal plants could provide scientific evidence for some traditional uses in the treatment of diseases related to the production of reactive oxygen species (ROS) and oxidative stress.

Authors+Show Affiliations

Laboratory of Natural Products, Department of Biology, Faculty of Sciences, Abou Bekr Belkaïd University, B. P 119, Tlemcen, 13000, Algeria. Electronic address: biofar23@yahoo.fr.Laboratory of Natural Products, Department of Biology, Faculty of Sciences, Abou Bekr Belkaïd University, B. P 119, Tlemcen, 13000, Algeria.Laboratory of Plant Adaptation to Abiotic Stresses, Biotechnologic Center in Borj-Cedria Technopol (CBBC), B. P 901, 2050 Hammam-Lif, Tunisia.Laboratory of Plant Adaptation to Abiotic Stresses, Biotechnologic Center in Borj-Cedria Technopol (CBBC), B. P 901, 2050 Hammam-Lif, Tunisia.Department of Biotechnology, Faculty of Natural Science and Life, Abdelel Hamid Ibn Badiss University, Mostaganem, 27000, Algeria.Laboratory of Natural Products, Department of Biology, Faculty of Sciences, Abou Bekr Belkaïd University, B. P 119, Tlemcen, 13000, Algeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24969521

Citation

Haddouchi, Farah, et al. "Antioxidant Activity Profiling By Spectrophotometric Methods of Aqueous Methanolic Extracts of Helichrysum Stoechas Subsp. Rupestre and Phagnalon Saxatile Subsp. Saxatile." Chinese Journal of Natural Medicines, vol. 12, no. 6, 2014, pp. 415-22.
Haddouchi F, Chaouche TM, Ksouri R, et al. Antioxidant activity profiling by spectrophotometric methods of aqueous methanolic extracts of Helichrysum stoechas subsp. rupestre and Phagnalon saxatile subsp. saxatile. Chin J Nat Med. 2014;12(6):415-22.
Haddouchi, F., Chaouche, T. M., Ksouri, R., Medini, F., Sekkal, F. Z., & Benmansour, A. (2014). Antioxidant activity profiling by spectrophotometric methods of aqueous methanolic extracts of Helichrysum stoechas subsp. rupestre and Phagnalon saxatile subsp. saxatile. Chinese Journal of Natural Medicines, 12(6), 415-22. https://doi.org/10.1016/S1875-5364(14)60065-0
Haddouchi F, et al. Antioxidant Activity Profiling By Spectrophotometric Methods of Aqueous Methanolic Extracts of Helichrysum Stoechas Subsp. Rupestre and Phagnalon Saxatile Subsp. Saxatile. Chin J Nat Med. 2014;12(6):415-22. PubMed PMID: 24969521.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activity profiling by spectrophotometric methods of aqueous methanolic extracts of Helichrysum stoechas subsp. rupestre and Phagnalon saxatile subsp. saxatile. AU - Haddouchi,Farah, AU - Chaouche,Tarik Mohammed, AU - Ksouri,Riadh, AU - Medini,Faten, AU - Sekkal,Fatima Zohra, AU - Benmansour,Abdelhafid, PY - 2013/03/20/received PY - 2014/6/28/entrez PY - 2014/6/28/pubmed PY - 2015/2/5/medline KW - Antioxidant activity KW - Helichrysum stoechas subsp. rupestre KW - Phagnalon saxatile subsp. saxatile KW - Phenolic compounds SP - 415 EP - 22 JF - Chinese journal of natural medicines JO - Chin J Nat Med VL - 12 IS - 6 N2 - AIM: The aqueous methanolic extracts of two plants from Algeria, Helichrysum stoechas subsp. rupestre and Phagnalon saxatile subsp. saxatile, were investigated for their antioxidant activity. METHOD: Total phenolics, flavonoids, and tannins were determined by spectrophotometric techniques. In vitro antioxidant and radical scavenging profiling was determined by spectrophotometric methods, through: Total antioxidant capacity, and radical scavenging effects by the DPPH and ABTS methods, reducing and chelating power, and blanching inhibition of the β-carotene. RESULTS: All of the extracts showed interesting antioxidant and radical scavenging activity. The highest contents in phenolics, tannins, and the highest total antioxidant capacity as gallic acid equivalents of 97.5 ± 0.33 mg GAE/g DW was obtained for the flowers of H. stoechas subsp. rupestre extract in the phosphomolybdenum assay. An extract of the leafy stems of P. saxatile subsp. saxatile revealed the highest content of flavonoids, and the highest antioxidant activity by the radical scavenging and β-carotene assays when compared with standards. The best activity was by the scavenging radical DPPH with an IC50 value of 5.65 ± 0.10 μg·mL(-1). CONCLUSION: The studied medicinal plants could provide scientific evidence for some traditional uses in the treatment of diseases related to the production of reactive oxygen species (ROS) and oxidative stress. SN - 1875-5364 UR - https://www.unboundmedicine.com/medline/citation/24969521/Antioxidant_activity_profiling_by_spectrophotometric_methods_of_aqueous_methanolic_extracts_of_Helichrysum_stoechas_subsp__rupestre_and_Phagnalon_saxatile_subsp__saxatile_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1875-5364(14)60065-0 DB - PRIME DP - Unbound Medicine ER -