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RRR-α-tocopheryl succinate induces apoptosis in human gastric cancer cells via the NF-κB signaling pathway.
Oncol Rep. 2014 Sep; 32(3):1243-8.OR

Abstract

To investigate the effects of the nuclear factor (NF)-κB signaling pathway on the induction of apoptosis by vitamin E succinate (RRR-α-tocopheryl succinate; VES) in human gastric carcinoma cells. Human gastric carcinoma SGC-7901 cells were treated with temperate concentrations of VES and pyrrolidine dithiocarbamate (PDTC), an inhibitor of NF-κB. Cell viability and apoptosis were respectively estimated by methylthiazol tetrazolium (MTT) assay and the Annexin V‑FITC method. Western blot analysis was used to evaluate the protein expressions of NF-κBp65 and Bcl-2 family members Bcl-2, Bax and cleavage of caspase-3, caspase-9, and poly (ADP-ribose) polymerase (PARP). The DNA-binding activity of NF-κBp65 was measured by electrophoretic mobility shift assay (EMSA). Reverse transcription and polymerase chain reaction (RT-PCR) was implemented to evaluate the transcription of inhibitor of apoptosis (IAP) genes. Apoptosis assessment showed that VES induces apoptotic cell death in human gastric carcinoma cells. In the following experiments, PDTC (100 µM) was used in cell treatment 2 h before VES. The decreased ratio of the nuclear and cytosolic NF-κBp65 protein level was induced by VES and PDTC reinforced this trend. PDTC treatment significantly enhanced the decrease of NF-κB-DNA binding activity induced by VES in human gastric SGC-7901. The decrease in protein expression of Bcl-2 as well as the increase in the protein expression of Bax were induced by VES treatment. The cleavage of caspase-9, caspase-3 and PARP was induced. There was no effect on the gene transcription of c-IAP-1, c-IAP-2, and x-linked IAP (XIAP) compared with the control group, whereas mRNA levels of survivin and the neuronal apoptosis inhibitory protein (NAIP) markedly decreased. Notably, pretreatment with PDTC reinforced all the above VES-induced effects. In conclusion, VES-induced apoptosis in SGC-7901 cells is accompanied by the inhibition of the NF-κB signaling pathway, including changes in Bcl-2 family members, cleavage of caspases and gene transcription of survivin and NAIP.

Authors+Show Affiliations

Department of Nutrition and Food, Harbin Medical University, Harbin, Heilongjiang, P.R. China.Department of Nutrition and Food, Harbin Medical University, Harbin, Heilongjiang, P.R. China.Department of Nutrition and Food, Harbin Medical University, Harbin, Heilongjiang, P.R. China.Department of Nutrition and Food, Harbin Medical University, Harbin, Heilongjiang, P.R. China.Food Processing Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, P.R. China.Department of Nutrition and Food, Harbin Medical University, Harbin, Heilongjiang, P.R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24970592

Citation

Sun, Yanpei, et al. "RRR-α-tocopheryl Succinate Induces Apoptosis in Human Gastric Cancer Cells Via the NF-κB Signaling Pathway." Oncology Reports, vol. 32, no. 3, 2014, pp. 1243-8.
Sun Y, Zhao Y, Hou L, et al. RRR-α-tocopheryl succinate induces apoptosis in human gastric cancer cells via the NF-κB signaling pathway. Oncol Rep. 2014;32(3):1243-8.
Sun, Y., Zhao, Y., Hou, L., Zhang, X., Zhang, Z., & Wu, K. (2014). RRR-α-tocopheryl succinate induces apoptosis in human gastric cancer cells via the NF-κB signaling pathway. Oncology Reports, 32(3), 1243-8. https://doi.org/10.3892/or.2014.3282
Sun Y, et al. RRR-α-tocopheryl Succinate Induces Apoptosis in Human Gastric Cancer Cells Via the NF-κB Signaling Pathway. Oncol Rep. 2014;32(3):1243-8. PubMed PMID: 24970592.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RRR-α-tocopheryl succinate induces apoptosis in human gastric cancer cells via the NF-κB signaling pathway. AU - Sun,Yanpei, AU - Zhao,Yan, AU - Hou,Liying, AU - Zhang,Xuguang, AU - Zhang,Zhihong, AU - Wu,Kun, Y1 - 2014/06/23/ PY - 2014/02/01/received PY - 2014/04/15/accepted PY - 2014/6/28/entrez PY - 2014/6/28/pubmed PY - 2015/11/4/medline SP - 1243 EP - 8 JF - Oncology reports JO - Oncol. Rep. VL - 32 IS - 3 N2 - To investigate the effects of the nuclear factor (NF)-κB signaling pathway on the induction of apoptosis by vitamin E succinate (RRR-α-tocopheryl succinate; VES) in human gastric carcinoma cells. Human gastric carcinoma SGC-7901 cells were treated with temperate concentrations of VES and pyrrolidine dithiocarbamate (PDTC), an inhibitor of NF-κB. Cell viability and apoptosis were respectively estimated by methylthiazol tetrazolium (MTT) assay and the Annexin V‑FITC method. Western blot analysis was used to evaluate the protein expressions of NF-κBp65 and Bcl-2 family members Bcl-2, Bax and cleavage of caspase-3, caspase-9, and poly (ADP-ribose) polymerase (PARP). The DNA-binding activity of NF-κBp65 was measured by electrophoretic mobility shift assay (EMSA). Reverse transcription and polymerase chain reaction (RT-PCR) was implemented to evaluate the transcription of inhibitor of apoptosis (IAP) genes. Apoptosis assessment showed that VES induces apoptotic cell death in human gastric carcinoma cells. In the following experiments, PDTC (100 µM) was used in cell treatment 2 h before VES. The decreased ratio of the nuclear and cytosolic NF-κBp65 protein level was induced by VES and PDTC reinforced this trend. PDTC treatment significantly enhanced the decrease of NF-κB-DNA binding activity induced by VES in human gastric SGC-7901. The decrease in protein expression of Bcl-2 as well as the increase in the protein expression of Bax were induced by VES treatment. The cleavage of caspase-9, caspase-3 and PARP was induced. There was no effect on the gene transcription of c-IAP-1, c-IAP-2, and x-linked IAP (XIAP) compared with the control group, whereas mRNA levels of survivin and the neuronal apoptosis inhibitory protein (NAIP) markedly decreased. Notably, pretreatment with PDTC reinforced all the above VES-induced effects. In conclusion, VES-induced apoptosis in SGC-7901 cells is accompanied by the inhibition of the NF-κB signaling pathway, including changes in Bcl-2 family members, cleavage of caspases and gene transcription of survivin and NAIP. SN - 1791-2431 UR - https://www.unboundmedicine.com/medline/citation/24970592/RRR_α_tocopheryl_succinate_induces_apoptosis_in_human_gastric_cancer_cells_via_the_NF_κB_signaling_pathway_ L2 - http://www.spandidos-publications.com/or/32/3/1243 DB - PRIME DP - Unbound Medicine ER -