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Fructose supplementation worsens the deleterious effects of short-term high-fat feeding on hepatic steatosis and lipid metabolism in adult rats.
Exp Physiol 2014; 99(9):1203-13EP

Abstract

The purpose of the present study was to examine the short-term effect of high-fat or high-fat-high-fructose feeding on hepatic lipid metabolism and mitochondrial function in adult sedentary rats. Adult male rats were fed a high-fat or high-fat-high-fructose diet for 2 weeks. Body and liver composition, hepatic steatosis, plasma lipid profile and hepatic insulin sensitivity, together with whole-body and hepatic de novo lipogenesis, were assessed. Hepatic mitochondrial mass, functionality, oxidative stress and antioxidant defense were also measured. Rats fed the high-fat-high-fructose diet exhibited significantly higher plasma triglycerides, non-esterified fatty acids, insulin and indexes of hepatic insulin resistance compared with rats fed a low-fat or a high-fat diet. Hepatic triglycerides and ceramide, as well as the degree of steatosis and necrosis, were significantly higher, while liver p-Akt was significantly lower, in rats fed high-fat-high-fructose diet than in rats fed high-fat diet. A significant increase in non-protein respiratory quotient and hepatic fatty acid synthase and stearoyl CoA desaturase activity was found in rats fed the high-fat-high-fructose diet compared with those fed the high-fat diet. Significantly lower mitochondrial oxidative capacity but significantly higher oxidative stress was found in rats fed high-fat and high-fat-high-fructose diets compared with rats fed low-fat diet, while mitochondrial mass significantly increased only in rats fed high-fat-high-fructose diet. In conclusion, short-term consumption of a Western diet, rich in saturated fats and fructose, is more conducive to the development of liver steatosis and deleterious to glucose homeostasis than a high-fat diet.

Authors+Show Affiliations

Department of Biology, University of Naples, Italy.Department of Biology, University of Naples, Italy.Department of Biology, University of Naples, Italy.Department of Biology, University of Naples, Italy.Department of Biology, University of Naples, Italy.Department of Biology, University of Naples, Italy.Department of Biology, University of Naples, Italy.Department of Biology, University of Naples, Italy susiossa@unina.it.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24972835

Citation

Crescenzo, Raffaella, et al. "Fructose Supplementation Worsens the Deleterious Effects of Short-term High-fat Feeding On Hepatic Steatosis and Lipid Metabolism in Adult Rats." Experimental Physiology, vol. 99, no. 9, 2014, pp. 1203-13.
Crescenzo R, Bianco F, Coppola P, et al. Fructose supplementation worsens the deleterious effects of short-term high-fat feeding on hepatic steatosis and lipid metabolism in adult rats. Exp Physiol. 2014;99(9):1203-13.
Crescenzo, R., Bianco, F., Coppola, P., Mazzoli, A., Tussellino, M., Carotenuto, R., ... Iossa, S. (2014). Fructose supplementation worsens the deleterious effects of short-term high-fat feeding on hepatic steatosis and lipid metabolism in adult rats. Experimental Physiology, 99(9), pp. 1203-13. doi:10.1113/expphysiol.2014.079632.
Crescenzo R, et al. Fructose Supplementation Worsens the Deleterious Effects of Short-term High-fat Feeding On Hepatic Steatosis and Lipid Metabolism in Adult Rats. Exp Physiol. 2014;99(9):1203-13. PubMed PMID: 24972835.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fructose supplementation worsens the deleterious effects of short-term high-fat feeding on hepatic steatosis and lipid metabolism in adult rats. AU - Crescenzo,Raffaella, AU - Bianco,Francesca, AU - Coppola,Paola, AU - Mazzoli,Arianna, AU - Tussellino,Margherita, AU - Carotenuto,Rosa, AU - Liverini,Giovanna, AU - Iossa,Susanna, Y1 - 2014/06/27/ PY - 2014/6/29/entrez PY - 2014/6/29/pubmed PY - 2015/7/3/medline SP - 1203 EP - 13 JF - Experimental physiology JO - Exp. Physiol. VL - 99 IS - 9 N2 - The purpose of the present study was to examine the short-term effect of high-fat or high-fat-high-fructose feeding on hepatic lipid metabolism and mitochondrial function in adult sedentary rats. Adult male rats were fed a high-fat or high-fat-high-fructose diet for 2 weeks. Body and liver composition, hepatic steatosis, plasma lipid profile and hepatic insulin sensitivity, together with whole-body and hepatic de novo lipogenesis, were assessed. Hepatic mitochondrial mass, functionality, oxidative stress and antioxidant defense were also measured. Rats fed the high-fat-high-fructose diet exhibited significantly higher plasma triglycerides, non-esterified fatty acids, insulin and indexes of hepatic insulin resistance compared with rats fed a low-fat or a high-fat diet. Hepatic triglycerides and ceramide, as well as the degree of steatosis and necrosis, were significantly higher, while liver p-Akt was significantly lower, in rats fed high-fat-high-fructose diet than in rats fed high-fat diet. A significant increase in non-protein respiratory quotient and hepatic fatty acid synthase and stearoyl CoA desaturase activity was found in rats fed the high-fat-high-fructose diet compared with those fed the high-fat diet. Significantly lower mitochondrial oxidative capacity but significantly higher oxidative stress was found in rats fed high-fat and high-fat-high-fructose diets compared with rats fed low-fat diet, while mitochondrial mass significantly increased only in rats fed high-fat-high-fructose diet. In conclusion, short-term consumption of a Western diet, rich in saturated fats and fructose, is more conducive to the development of liver steatosis and deleterious to glucose homeostasis than a high-fat diet. SN - 1469-445X UR - https://www.unboundmedicine.com/medline/citation/24972835/Fructose_supplementation_worsens_the_deleterious_effects_of_short_term_high_fat_feeding_on_hepatic_steatosis_and_lipid_metabolism_in_adult_rats_ L2 - https://doi.org/10.1113/expphysiol.2014.079632 DB - PRIME DP - Unbound Medicine ER -