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The virion of Cafeteria roenbergensis virus (CroV) contains a complex suite of proteins for transcription and DNA repair.
Virology. 2014 Oct; 466-467:82-94.V

Abstract

Cafeteria roenbergensis virus (CroV) is a giant virus of the Mimiviridae family that infects the marine phagotrophic flagellate C. roenbergensis. CroV possesses a DNA genome of ~730 kilobase pairs that is predicted to encode 544 proteins. We analyzed the protein composition of purified CroV particles by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and identified 141 virion-associated CroV proteins and 60 host proteins. Data are available via ProteomeXchange with identifier PXD000993. Predicted functions could be assigned to 36% of the virion proteins, which include structural proteins as well as enzymes for transcription, DNA repair, redox reactions and protein modification. Homologs of 36 CroV virion proteins have previously been found in the virion of Acanthamoeba polyphaga mimivirus. The overlapping virion proteome of CroV and Mimivirus reveals a set of conserved virion protein functions that were presumably present in the last common ancestor of the Mimiviridae.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Fischer MG
Department of Microbiology & Immunology, University of British Columbia, Vancouver, Canada V6T 1Z4. Electronic address: mfischer@mpimf-heidelberg.mpg.de.
Kelly I
Department of Biochemistry & Molecular Biology and Centre for High-Throughput Biology, University of British Columbia, Vancouver, Canada V6T 1Z4.
Foster LJ
Department of Biochemistry & Molecular Biology and Centre for High-Throughput Biology, University of British Columbia, Vancouver, Canada V6T 1Z4.
Suttle CA
Department of Microbiology & Immunology, University of British Columbia, Vancouver, Canada V6T 1Z4; Department of Botany, University of British Columbia, Vancouver, Canada V6T 1Z4; Department of Earth & Ocean Sciences, University of British Columbia, Vancouver, Canada V6T 1Z4.

MeSH

Amino Acid SequenceChromatography, LiquidDNA RepairDNA, ViralGenome, ViralMimiviridaeMolecular Sequence DataNucleotide MotifsOxidation-ReductionPromoter Regions, GeneticProteomeStramenopilesTandem Mass SpectrometryTranscription, GeneticViral ProteinsViral StructuresVirion

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24973308

Citation

Fischer, Matthias G., et al. "The Virion of Cafeteria Roenbergensis Virus (CroV) Contains a Complex Suite of Proteins for Transcription and DNA Repair." Virology, vol. 466-467, 2014, pp. 82-94.
Fischer MG, Kelly I, Foster LJ, et al. The virion of Cafeteria roenbergensis virus (CroV) contains a complex suite of proteins for transcription and DNA repair. Virology. 2014;466-467:82-94.
Fischer, M. G., Kelly, I., Foster, L. J., & Suttle, C. A. (2014). The virion of Cafeteria roenbergensis virus (CroV) contains a complex suite of proteins for transcription and DNA repair. Virology, 466-467, 82-94. https://doi.org/10.1016/j.virol.2014.05.029
Fischer MG, et al. The Virion of Cafeteria Roenbergensis Virus (CroV) Contains a Complex Suite of Proteins for Transcription and DNA Repair. Virology. 2014;466-467:82-94. PubMed PMID: 24973308.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The virion of Cafeteria roenbergensis virus (CroV) contains a complex suite of proteins for transcription and DNA repair. AU - Fischer,Matthias G, AU - Kelly,Isabelle, AU - Foster,Leonard J, AU - Suttle,Curtis A, Y1 - 2014/06/25/ PY - 2014/05/13/received PY - 2014/05/25/revised PY - 2014/05/27/accepted PY - 2014/6/29/entrez PY - 2014/6/29/pubmed PY - 2015/3/11/medline KW - Cafeteria roenbergensis virus KW - CroV KW - Giant virus KW - LC–MS/MS KW - Megavirales KW - Mimivirus KW - NCLDV KW - Proteome KW - Virion KW - Virophage SP - 82 EP - 94 JF - Virology JO - Virology VL - 466-467 N2 - Cafeteria roenbergensis virus (CroV) is a giant virus of the Mimiviridae family that infects the marine phagotrophic flagellate C. roenbergensis. CroV possesses a DNA genome of ~730 kilobase pairs that is predicted to encode 544 proteins. We analyzed the protein composition of purified CroV particles by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and identified 141 virion-associated CroV proteins and 60 host proteins. Data are available via ProteomeXchange with identifier PXD000993. Predicted functions could be assigned to 36% of the virion proteins, which include structural proteins as well as enzymes for transcription, DNA repair, redox reactions and protein modification. Homologs of 36 CroV virion proteins have previously been found in the virion of Acanthamoeba polyphaga mimivirus. The overlapping virion proteome of CroV and Mimivirus reveals a set of conserved virion protein functions that were presumably present in the last common ancestor of the Mimiviridae. SN - 1096-0341 UR - https://www.unboundmedicine.com/medline/citation/24973308/The_virion_of_Cafeteria_roenbergensis_virus__CroV__contains_a_complex_suite_of_proteins_for_transcription_and_DNA_repair_ DB - PRIME DP - Unbound Medicine ER -