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Egr-1 mRNA expression patterns in the prefrontal cortex, hippocampus, and amygdala during variants of contextual fear conditioning in adolescent rats.
Brain Res. 2014 Aug 12; 1576:63-72.BR

Abstract

We report activation of the immediate-early gene Egr-1 in the lateral amygdala (LA), hippocampus (CA1), and medial prefrontal cortex (mPFC) 30-min following the training phase in the context pre-exposure facilitation effect (CPFE) and standard context fear conditioning (180 s context exposure→shock). On day one of the CPFE paradigm, postnatal day (PD) 31 rats (±1) were pre-exposed to Context A (Pre) or Context B (Alt-Pre) for 5 min followed by five additional 1-min exposures. A day later, Pre and Alt-Pre rats received a 2-s, 1.5 mA footshock immediately upon placement in Context A. Animals included in in situ hybridization were then sacrificed 30 (±3) min later. On day three, the behaviorally-tested Pre rats showed significantly more fear-conditioned freezing in Context A than Alt-Pre rats. Standard context fear conditioning groups showed much greater freezing than the Pre group, as well as no shock and immediate-shock controls. Thirty minutes after immediate shock training, Pre rats showed increased Egr-1 mRNA in the prelimbic mPFC relative to Alt-Pre rats. Standard context conditioning selectively increased Egr-1 in CA1. In the LA and mPFC, Egr-1 increased to a similar extent in no shock, immediate shock, and standard context conditioning relative to homecage controls. The present study demonstrates that Egr-1 mRNA expression has a complex relationship to fear learning in different brain regions and variants of context conditioning.

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Authors+Show Affiliations

Schreiber WB
Department of Psychological & Brain Sciences, University of Delaware, Newark, DE 19716, USA.
Asok A
Department of Psychological & Brain Sciences, University of Delaware, Newark, DE 19716, USA.
Jablonski SA
Department of Psychological & Brain Sciences, University of Delaware, Newark, DE 19716, USA.
Rosen JB
Department of Psychological & Brain Sciences, University of Delaware, Newark, DE 19716, USA.
Stanton ME
Department of Psychological & Brain Sciences, University of Delaware, Newark, DE 19716, USA. Electronic address: stanton@udel.edu.

MeSH

Age FactorsAmygdalaAnimalsConditioning, ClassicalEarly Growth Response Protein 1ElectroshockFearFemaleFreezing Reaction, CatalepticGenes, Immediate-EarlyHandling, PsychologicalHippocampusMaleNerve Tissue ProteinsNeuronal PlasticityPrefrontal CortexRNA, MessengerRatsRats, Long-EvansTime Factors

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24976583

Citation

Schreiber, W B., et al. "Egr-1 mRNA Expression Patterns in the Prefrontal Cortex, Hippocampus, and Amygdala During Variants of Contextual Fear Conditioning in Adolescent Rats." Brain Research, vol. 1576, 2014, pp. 63-72.
Schreiber WB, Asok A, Jablonski SA, et al. Egr-1 mRNA expression patterns in the prefrontal cortex, hippocampus, and amygdala during variants of contextual fear conditioning in adolescent rats. Brain Res. 2014;1576:63-72.
Schreiber, W. B., Asok, A., Jablonski, S. A., Rosen, J. B., & Stanton, M. E. (2014). Egr-1 mRNA expression patterns in the prefrontal cortex, hippocampus, and amygdala during variants of contextual fear conditioning in adolescent rats. Brain Research, 1576, 63-72. https://doi.org/10.1016/j.brainres.2014.06.007
Schreiber WB, et al. Egr-1 mRNA Expression Patterns in the Prefrontal Cortex, Hippocampus, and Amygdala During Variants of Contextual Fear Conditioning in Adolescent Rats. Brain Res. 2014 Aug 12;1576:63-72. PubMed PMID: 24976583.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Egr-1 mRNA expression patterns in the prefrontal cortex, hippocampus, and amygdala during variants of contextual fear conditioning in adolescent rats. AU - Schreiber,W B, AU - Asok,A, AU - Jablonski,S A, AU - Rosen,J B, AU - Stanton,M E, Y1 - 2014/06/26/ PY - 2013/12/19/received PY - 2014/06/03/revised PY - 2014/06/05/accepted PY - 2014/7/1/entrez PY - 2014/7/1/pubmed PY - 2015/4/1/medline KW - Amygdala KW - CPFE KW - Context fear conditioning KW - Egr-1 KW - Hippocampus KW - Prefrontal cortex SP - 63 EP - 72 JF - Brain research JO - Brain Res VL - 1576 N2 - We report activation of the immediate-early gene Egr-1 in the lateral amygdala (LA), hippocampus (CA1), and medial prefrontal cortex (mPFC) 30-min following the training phase in the context pre-exposure facilitation effect (CPFE) and standard context fear conditioning (180 s context exposure→shock). On day one of the CPFE paradigm, postnatal day (PD) 31 rats (±1) were pre-exposed to Context A (Pre) or Context B (Alt-Pre) for 5 min followed by five additional 1-min exposures. A day later, Pre and Alt-Pre rats received a 2-s, 1.5 mA footshock immediately upon placement in Context A. Animals included in in situ hybridization were then sacrificed 30 (±3) min later. On day three, the behaviorally-tested Pre rats showed significantly more fear-conditioned freezing in Context A than Alt-Pre rats. Standard context fear conditioning groups showed much greater freezing than the Pre group, as well as no shock and immediate-shock controls. Thirty minutes after immediate shock training, Pre rats showed increased Egr-1 mRNA in the prelimbic mPFC relative to Alt-Pre rats. Standard context conditioning selectively increased Egr-1 in CA1. In the LA and mPFC, Egr-1 increased to a similar extent in no shock, immediate shock, and standard context conditioning relative to homecage controls. The present study demonstrates that Egr-1 mRNA expression has a complex relationship to fear learning in different brain regions and variants of context conditioning. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/24976583/Egr_1_mRNA_expression_patterns_in_the_prefrontal_cortex_hippocampus_and_amygdala_during_variants_of_contextual_fear_conditioning_in_adolescent_rats_ DB - PRIME DP - Unbound Medicine ER -