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One or 4 h of "in-house" reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneys.
Transpl Int. 2014 Nov; 27(11):1214-9.TI

Abstract

In-house machine perfusion after cold storage (hypothermic reconditioning) has been proposed as convenient tool to improve kidney graft function. This study investigated the role of machine perfusion duration for early reperfusion parameters in porcine kidneys. Kidney function after cold preservation (4 °C, 18 h) and subsequent reconditioning by one or 4 h of pulsatile, nonoxygenated hypothermic machine perfusion (HMP) was studied in an isolated kidney perfusion model in pigs (n = 6, respectively) and compared with simply cold-stored grafts (CS). Compared with CS alone, one or 4 h of subsequent HMP similarly and significantly improved renal flow and kidney function (clearance and sodium reabsorption) upon warm reperfusion, along with reduced perfusate concentrations of endothelin-1 and increased vascular release of nitric oxide. Molecular effects of HMP comprised a significant (vs CS) mRNA increase in the endothelial transcription factor KLF2 and lower expression of endothelin that were observed already at the end of one-hour HMP after CS. Reconditioning of cold-stored kidneys is possible, even if clinical logistics only permit one hour of therapy, while limited extension of the overall storage time by in-house machine perfusion might also allow for postponing of transplantation from night to early day work.

Authors+Show Affiliations

Clinic of General, Visceral and Transplantation Surgery, University Hospital of Essen, Essen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

24977654

Citation

Gallinat, Anja, et al. "One or 4 H of "in-house" Reconditioning By Machine Perfusion After Cold Storage Improve Reperfusion Parameters in Porcine Kidneys." Transplant International : Official Journal of the European Society for Organ Transplantation, vol. 27, no. 11, 2014, pp. 1214-9.
Gallinat A, Efferz P, Paul A, et al. One or 4 h of "in-house" reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneys. Transpl Int. 2014;27(11):1214-9.
Gallinat, A., Efferz, P., Paul, A., & Minor, T. (2014). One or 4 h of "in-house" reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneys. Transplant International : Official Journal of the European Society for Organ Transplantation, 27(11), 1214-9. https://doi.org/10.1111/tri.12393
Gallinat A, et al. One or 4 H of "in-house" Reconditioning By Machine Perfusion After Cold Storage Improve Reperfusion Parameters in Porcine Kidneys. Transpl Int. 2014;27(11):1214-9. PubMed PMID: 24977654.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - One or 4 h of "in-house" reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneys. AU - Gallinat,Anja, AU - Efferz,Patrik, AU - Paul,Andreas, AU - Minor,Thomas, Y1 - 2014/08/20/ PY - 2014/03/29/received PY - 2014/04/22/revised PY - 2014/06/26/accepted PY - 2014/7/1/entrez PY - 2014/7/1/pubmed PY - 2015/6/30/medline KW - machine perfusion KW - reconditioning KW - renovasculature KW - time SP - 1214 EP - 9 JF - Transplant international : official journal of the European Society for Organ Transplantation JO - Transpl Int VL - 27 IS - 11 N2 - In-house machine perfusion after cold storage (hypothermic reconditioning) has been proposed as convenient tool to improve kidney graft function. This study investigated the role of machine perfusion duration for early reperfusion parameters in porcine kidneys. Kidney function after cold preservation (4 °C, 18 h) and subsequent reconditioning by one or 4 h of pulsatile, nonoxygenated hypothermic machine perfusion (HMP) was studied in an isolated kidney perfusion model in pigs (n = 6, respectively) and compared with simply cold-stored grafts (CS). Compared with CS alone, one or 4 h of subsequent HMP similarly and significantly improved renal flow and kidney function (clearance and sodium reabsorption) upon warm reperfusion, along with reduced perfusate concentrations of endothelin-1 and increased vascular release of nitric oxide. Molecular effects of HMP comprised a significant (vs CS) mRNA increase in the endothelial transcription factor KLF2 and lower expression of endothelin that were observed already at the end of one-hour HMP after CS. Reconditioning of cold-stored kidneys is possible, even if clinical logistics only permit one hour of therapy, while limited extension of the overall storage time by in-house machine perfusion might also allow for postponing of transplantation from night to early day work. SN - 1432-2277 UR - https://www.unboundmedicine.com/medline/citation/24977654/One_or_4_h_of_"in_house"_reconditioning_by_machine_perfusion_after_cold_storage_improve_reperfusion_parameters_in_porcine_kidneys_ L2 - https://doi.org/10.1111/tri.12393 DB - PRIME DP - Unbound Medicine ER -