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Cloning and characterization of low-molecular-weight glutenin subunit alleles from Chinese wheat landraces (Triticum aestivum L.).
ScientificWorldJournal. 2014; 2014:371045.S

Abstract

Low-molecular-weight glutenin subunits (LMW-GS) are of great importance in processing quality and participate in the formation of polymers in wheat. In this study, eight new LMW-GS alleles were isolated from Chinese wheat landraces (Triticum aestivum L.) and designated as Glu-A3-1a, Glu-A3-1b, Glu-B3-1a, Glu-B3-1b, Glu-B3-1c, Glu-D3-1a, Glu-D3-1b, and Glu-D3-1c, which were located at the Glu-A3, Glu-B3, and Glu-D3 loci, respectively. Based on the proteins encoded, the number of deduced amino acids of Glu-B3 alleles was approximately 50 more than those of Glu-A3 and Glu-D3 alleles. The first cysteine of Glu-A3 and Glu-D3 alleles was located at the N-terminal domain, while that of Glu-B3 alleles was found in the repetitive domain, which may lead to the different functioning in forming disulfide bonds. All the eight genes were LMW-m types and the new allele of Glu-B3-1a which had nine cysteine residues may be the desirable LMW-GS gene for improving bread-making quality.

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Authors+Show Affiliations

Si H
School of Agronomy, Anhui Agricultural University, Hefei 230036, China ; Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036, China.
Zhao M
School of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Zhang X
School of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Yao G
School of Agronomy, Anhui Agricultural University, Hefei 230036, China.
Sun G
School of Agronomy, Anhui Agricultural University, Hefei 230036, China ; Biology Department, Saint Mary's University, Halifax, NS, Canada B3H 3C3.
Ma C
School of Agronomy, Anhui Agricultural University, Hefei 230036, China ; Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036, China.

MeSH

AllelesGlutensPlant ProteinsSequence Analysis, DNATriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24982945

Citation

Si, Hongqi, et al. "Cloning and Characterization of Low-molecular-weight Glutenin Subunit Alleles From Chinese Wheat Landraces (Triticum Aestivum L.)." TheScientificWorldJournal, vol. 2014, 2014, p. 371045.
Si H, Zhao M, Zhang X, et al. Cloning and characterization of low-molecular-weight glutenin subunit alleles from Chinese wheat landraces (Triticum aestivum L.). ScientificWorldJournal. 2014;2014:371045.
Si, H., Zhao, M., Zhang, X., Yao, G., Sun, G., & Ma, C. (2014). Cloning and characterization of low-molecular-weight glutenin subunit alleles from Chinese wheat landraces (Triticum aestivum L.). TheScientificWorldJournal, 2014, 371045. https://doi.org/10.1155/2014/371045
Si H, et al. Cloning and Characterization of Low-molecular-weight Glutenin Subunit Alleles From Chinese Wheat Landraces (Triticum Aestivum L.). ScientificWorldJournal. 2014;2014:371045. PubMed PMID: 24982945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and characterization of low-molecular-weight glutenin subunit alleles from Chinese wheat landraces (Triticum aestivum L.). AU - Si,Hongqi, AU - Zhao,Manli, AU - Zhang,Xin, AU - Yao,Guoliang, AU - Sun,Genlou, AU - Ma,Chuanxi, Y1 - 2014/04/10/ PY - 2014/02/13/received PY - 2014/03/19/accepted PY - 2014/7/2/entrez PY - 2014/7/2/pubmed PY - 2015/4/9/medline SP - 371045 EP - 371045 JF - TheScientificWorldJournal JO - ScientificWorldJournal VL - 2014 N2 - Low-molecular-weight glutenin subunits (LMW-GS) are of great importance in processing quality and participate in the formation of polymers in wheat. In this study, eight new LMW-GS alleles were isolated from Chinese wheat landraces (Triticum aestivum L.) and designated as Glu-A3-1a, Glu-A3-1b, Glu-B3-1a, Glu-B3-1b, Glu-B3-1c, Glu-D3-1a, Glu-D3-1b, and Glu-D3-1c, which were located at the Glu-A3, Glu-B3, and Glu-D3 loci, respectively. Based on the proteins encoded, the number of deduced amino acids of Glu-B3 alleles was approximately 50 more than those of Glu-A3 and Glu-D3 alleles. The first cysteine of Glu-A3 and Glu-D3 alleles was located at the N-terminal domain, while that of Glu-B3 alleles was found in the repetitive domain, which may lead to the different functioning in forming disulfide bonds. All the eight genes were LMW-m types and the new allele of Glu-B3-1a which had nine cysteine residues may be the desirable LMW-GS gene for improving bread-making quality. SN - 1537-744X UR - https://www.unboundmedicine.com/medline/citation/24982945/Cloning_and_characterization_of_low_molecular_weight_glutenin_subunit_alleles_from_Chinese_wheat_landraces__Triticum_aestivum_L___ DB - PRIME DP - Unbound Medicine ER -