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Resveratrol attenuates hepatic steatosis in high-fat fed mice by decreasing lipogenesis and inflammation.
Nutrition 2014 Jul-Aug; 30(7-8):915-9N

Abstract

OBJECTIVE

Resveratrol (RSV) is the most studied natural compound that activates sirtuins, which produce beneficial metabolic effects on lipid and glucose metabolism. The aim of the present study was to investigate the role of resveratrol in preventing nonalcoholic fatty liver disease (NAFLD) and expression of liver inflammatory markers in mice treated with a high-fat diet.

METHODS AND PROCEDURES

Eighteen male mice were divided into three groups and fed for 60 d with a standard diet (ST), high-fat diet (HFD), or high-fat diet plus resveratrol (HFD + RSV, 30 mg/kg/d). Body weight, food intake, and serum total cholesterol, triacylglycerol, insulin, alanine transaminase (ALT), and aspartate aminotransferase (AST) were evaluated. Liver histology was analyzed. Expression of ACC, PPAR-γ, ChREBP, SREBP-1 c, CPT-1, tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), NF-κB, interleukin 1 β (IL-1 β), and SIRT1 were evaluated by quantitative real-time reverse transcriptase PCR (qRT-PCR).

RESULTS

The major finding of the present study was that RSV reduced body fat, total cholesterol, triacylglycerol, transaminases, and insulin plasma level. These results were accompanied with a significant reduction in TNF-α, IL-6, and NF-κB mRNA expression in the liver. Analyses of liver adipogenesis related genes indicated that ACC, PPAR-γ, and SREBP-1 mRNA expression were significantly suppressed in HFD + RSV mice. In addition, we observed increased expression of SIRT1 in the HFD + RSV group.

CONCLUSIONS

We observed that treatment with resveratrol improved lipid metabolism, and decreased NAFLD and pro-inflammatory profile in liver of mice with obesity-inducible diets. These data suggest an important clinical application of RSV in preventing liver diseases.

Authors+Show Affiliations

Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil; Nursing Department, Faculdades Integradas Pitágoras, Montes Claros, Minas Gerais, Brazil.Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.Touro University Nevada College of Osteopathic and Medicine, Henderson, Las Vegas, Nevada, USA.Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil; Institute of Biological Sciences, Department of Pharmacology, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address: Sergiosousas@hotmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24985011

Citation

Andrade, João Marcus Oliveira, et al. "Resveratrol Attenuates Hepatic Steatosis in High-fat Fed Mice By Decreasing Lipogenesis and Inflammation." Nutrition (Burbank, Los Angeles County, Calif.), vol. 30, no. 7-8, 2014, pp. 915-9.
Andrade JM, Paraíso AF, de Oliveira MV, et al. Resveratrol attenuates hepatic steatosis in high-fat fed mice by decreasing lipogenesis and inflammation. Nutrition. 2014;30(7-8):915-9.
Andrade, J. M., Paraíso, A. F., de Oliveira, M. V., Martins, A. M., Neto, J. F., Guimarães, A. L., ... Santos, S. H. (2014). Resveratrol attenuates hepatic steatosis in high-fat fed mice by decreasing lipogenesis and inflammation. Nutrition (Burbank, Los Angeles County, Calif.), 30(7-8), pp. 915-9. doi:10.1016/j.nut.2013.11.016.
Andrade JM, et al. Resveratrol Attenuates Hepatic Steatosis in High-fat Fed Mice By Decreasing Lipogenesis and Inflammation. Nutrition. 2014;30(7-8):915-9. PubMed PMID: 24985011.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Resveratrol attenuates hepatic steatosis in high-fat fed mice by decreasing lipogenesis and inflammation. AU - Andrade,João Marcus Oliveira, AU - Paraíso,Alanna Fernandes, AU - de Oliveira,Marcos Vinícius Macedo, AU - Martins,Andréa Maria Eleutério, AU - Neto,João Felício, AU - Guimarães,André Luiz Sena, AU - de Paula,Alfredo Maurício, AU - Qureshi,Mahboob, AU - Santos,Sérgio Henrique Sousa, Y1 - 2013/12/04/ PY - 2013/09/19/received PY - 2013/11/01/revised PY - 2013/11/20/accepted PY - 2014/7/3/entrez PY - 2014/7/6/pubmed PY - 2015/2/11/medline KW - Inflammation KW - Nonalcoholic fatty liver disease KW - Obesity KW - Resveratrol KW - SIRT1 SP - 915 EP - 9 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 30 IS - 7-8 N2 - OBJECTIVE: Resveratrol (RSV) is the most studied natural compound that activates sirtuins, which produce beneficial metabolic effects on lipid and glucose metabolism. The aim of the present study was to investigate the role of resveratrol in preventing nonalcoholic fatty liver disease (NAFLD) and expression of liver inflammatory markers in mice treated with a high-fat diet. METHODS AND PROCEDURES: Eighteen male mice were divided into three groups and fed for 60 d with a standard diet (ST), high-fat diet (HFD), or high-fat diet plus resveratrol (HFD + RSV, 30 mg/kg/d). Body weight, food intake, and serum total cholesterol, triacylglycerol, insulin, alanine transaminase (ALT), and aspartate aminotransferase (AST) were evaluated. Liver histology was analyzed. Expression of ACC, PPAR-γ, ChREBP, SREBP-1 c, CPT-1, tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), NF-κB, interleukin 1 β (IL-1 β), and SIRT1 were evaluated by quantitative real-time reverse transcriptase PCR (qRT-PCR). RESULTS: The major finding of the present study was that RSV reduced body fat, total cholesterol, triacylglycerol, transaminases, and insulin plasma level. These results were accompanied with a significant reduction in TNF-α, IL-6, and NF-κB mRNA expression in the liver. Analyses of liver adipogenesis related genes indicated that ACC, PPAR-γ, and SREBP-1 mRNA expression were significantly suppressed in HFD + RSV mice. In addition, we observed increased expression of SIRT1 in the HFD + RSV group. CONCLUSIONS: We observed that treatment with resveratrol improved lipid metabolism, and decreased NAFLD and pro-inflammatory profile in liver of mice with obesity-inducible diets. These data suggest an important clinical application of RSV in preventing liver diseases. SN - 1873-1244 UR - https://www.unboundmedicine.com/medline/citation/24985011/Resveratrol_attenuates_hepatic_steatosis_in_high_fat_fed_mice_by_decreasing_lipogenesis_and_inflammation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0899-9007(13)00544-3 DB - PRIME DP - Unbound Medicine ER -