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Modification by UV radiation of the surface of thin films based on collagen extracted from fish scales.
Biointerphases. 2014 Jun; 9(2):029003.B

Abstract

Collagen was extracted from fish scales (Esox lucius) through demineralization process. Thin films by solvent evaporation from collagen extracted from fish scales were prepared. The surface of thin films made of fish scales collagen was modified by ultraviolet (UV)-irradiation with the wavelength λ = 254 nm. The amino acid composition of the Esox lucius scale collagen was analyzed before and after UV-irradiation by means of high-pressure liquid chromatography. The surface properties of films were investigated using the technique of atomic force microscopy (AFM) and by means of contact angle measurements allowing the calculation of surface free energy. Measurements of the contact angle for diiodomethane (D) and glycerol (G) on the surface of fish collagen films were made and surface free energy was calculated. The structure of collagen before and after UV-irradiation was studied using Fourier-transform infrared spectroscopy. It was found that after UV-irradiation the amount of all amino acids present in collagen molecule decreased. It was found also that the contact angle and the surface free energy were altered by UV-irradiation of collagen film. AFM showed that the surface roughness of collagen films was also altered by UV-irradiation. UV-irradiation caused the decrease of surface roughness due to photochemical processes, which occurred in the top layer of collagen film. The formation of collagen fibrils after solvent evaporation was observed using AFM. The diameter of collagen fibrils was bigger for irradiated collagen film than the diameter of collagen fibrils before UV-irradiation.

Authors+Show Affiliations

Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, Poland.Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, Poland.ISM UMR 5255, Université de Bordeaux, 351 Cours de la Libération, F-33405 Talence, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24985207

Citation

Sionkowska, Alina, et al. "Modification By UV Radiation of the Surface of Thin Films Based On Collagen Extracted From Fish Scales." Biointerphases, vol. 9, no. 2, 2014, p. 029003.
Sionkowska A, Kozłowska J, Lazare S. Modification by UV radiation of the surface of thin films based on collagen extracted from fish scales. Biointerphases. 2014;9(2):029003.
Sionkowska, A., Kozłowska, J., & Lazare, S. (2014). Modification by UV radiation of the surface of thin films based on collagen extracted from fish scales. Biointerphases, 9(2), 029003. https://doi.org/10.1116/1.4865753
Sionkowska A, Kozłowska J, Lazare S. Modification By UV Radiation of the Surface of Thin Films Based On Collagen Extracted From Fish Scales. Biointerphases. 2014;9(2):029003. PubMed PMID: 24985207.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modification by UV radiation of the surface of thin films based on collagen extracted from fish scales. AU - Sionkowska,Alina, AU - Kozłowska,Justyna, AU - Lazare,Sylvain, PY - 2014/7/3/entrez PY - 2014/7/6/pubmed PY - 2015/2/11/medline SP - 029003 EP - 029003 JF - Biointerphases JO - Biointerphases VL - 9 IS - 2 N2 - Collagen was extracted from fish scales (Esox lucius) through demineralization process. Thin films by solvent evaporation from collagen extracted from fish scales were prepared. The surface of thin films made of fish scales collagen was modified by ultraviolet (UV)-irradiation with the wavelength λ = 254 nm. The amino acid composition of the Esox lucius scale collagen was analyzed before and after UV-irradiation by means of high-pressure liquid chromatography. The surface properties of films were investigated using the technique of atomic force microscopy (AFM) and by means of contact angle measurements allowing the calculation of surface free energy. Measurements of the contact angle for diiodomethane (D) and glycerol (G) on the surface of fish collagen films were made and surface free energy was calculated. The structure of collagen before and after UV-irradiation was studied using Fourier-transform infrared spectroscopy. It was found that after UV-irradiation the amount of all amino acids present in collagen molecule decreased. It was found also that the contact angle and the surface free energy were altered by UV-irradiation of collagen film. AFM showed that the surface roughness of collagen films was also altered by UV-irradiation. UV-irradiation caused the decrease of surface roughness due to photochemical processes, which occurred in the top layer of collagen film. The formation of collagen fibrils after solvent evaporation was observed using AFM. The diameter of collagen fibrils was bigger for irradiated collagen film than the diameter of collagen fibrils before UV-irradiation. SN - 1559-4106 UR - https://www.unboundmedicine.com/medline/citation/24985207/Modification_by_UV_radiation_of_the_surface_of_thin_films_based_on_collagen_extracted_from_fish_scales_ DB - PRIME DP - Unbound Medicine ER -