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Comparative studies of the venom of a new Taipan species, Oxyuranus temporalis, with other members of its genus.
Toxins (Basel). 2014 Jul 02; 6(7):1979-95.T

Abstract

Taipans are highly venomous Australo-Papuan elapids. A new species of taipan, the Western Desert Taipan (Oxyuranus temporalis), has been discovered with two specimens housed in captivity at the Adelaide Zoo. This study is the first investigation of O. temporalis venom and seeks to characterise and compare the neurotoxicity, lethality and biochemical properties of O. temporalis venom with other taipan venoms. Analysis of O. temporalis venom using size-exclusion and reverse-phase HPLC indicated a markedly simplified "profile" compared to other taipan venoms. SDS-PAGE and agarose gel electrophoresis analysis also indicated a relatively simple composition. Murine LD50 studies showed that O. temporalis venom is less lethal than O. microlepidotus venom. Venoms were tested in vitro, using the chick biventer cervicis nerve-muscle preparation. Based on t90 values, O. temporalis venom is highly neurotoxic abolishing indirect twitches far more rapidly than other taipan venoms. O. temporalis venom also abolished responses to exogenous acetylcholine and carbachol, indicating the presence of postsynaptic neurotoxins. Prior administration of CSL Taipan antivenom (CSL Limited) neutralised the inhibitory effects of all taipan venoms. The results of this study suggest that the venom of the O. temporalis is highly neurotoxic in vitro and may contain procoagulant toxins, making this snake potentially dangerous to humans.

Authors+Show Affiliations

Monash Venom Group, Department of Pharmacology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Victoria 3168, Australia. carmel.barber@monash.edu.Venom Science Pty Ltd, Tanunda, South Australia 5352, Australia. frankmadaras@esc.net.au.SA Pathology, IMVS Veterinary Services, Gilles Plains, South Australia 5086, Australia. randcturnbull@bigpond.com.Adelaide Zoo, Adelaide, South Australia 5000, Australia. tmorley@zoossa.com.au.Venom Supplies, Tanunda, South Australia, South Australia 5352, Australia. luke@venomsupplies.com.Venom Supplies, Tanunda, South Australia, South Australia 5352, Australia. nathan@venomsupplies.com.SA Pathology, IMVS Veterinary Services, Gilles Plains, South Australia 5086, Australia. tim.kuchel@sahmri.com.Venom Science Pty Ltd, Tanunda, South Australia 5352, Australia. peter@venomsupplies.com.Monash Venom Group, Department of Pharmacology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Victoria 3168, Australia. wayne.hodgson@monash.edu.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24992081

Citation

Barber, Carmel M., et al. "Comparative Studies of the Venom of a New Taipan Species, Oxyuranus Temporalis, With Other Members of Its Genus." Toxins, vol. 6, no. 7, 2014, pp. 1979-95.
Barber CM, Madaras F, Turnbull RK, et al. Comparative studies of the venom of a new Taipan species, Oxyuranus temporalis, with other members of its genus. Toxins (Basel). 2014;6(7):1979-95.
Barber, C. M., Madaras, F., Turnbull, R. K., Morley, T., Dunstan, N., Allen, L., Kuchel, T., Mirtschin, P., & Hodgson, W. C. (2014). Comparative studies of the venom of a new Taipan species, Oxyuranus temporalis, with other members of its genus. Toxins, 6(7), 1979-95. https://doi.org/10.3390/toxins6071979
Barber CM, et al. Comparative Studies of the Venom of a New Taipan Species, Oxyuranus Temporalis, With Other Members of Its Genus. Toxins (Basel). 2014 Jul 2;6(7):1979-95. PubMed PMID: 24992081.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative studies of the venom of a new Taipan species, Oxyuranus temporalis, with other members of its genus. AU - Barber,Carmel M, AU - Madaras,Frank, AU - Turnbull,Richard K, AU - Morley,Terry, AU - Dunstan,Nathan, AU - Allen,Luke, AU - Kuchel,Tim, AU - Mirtschin,Peter, AU - Hodgson,Wayne C, Y1 - 2014/07/02/ PY - 2014/05/05/received PY - 2014/06/11/revised PY - 2014/06/16/accepted PY - 2014/7/4/entrez PY - 2014/7/6/pubmed PY - 2015/2/5/medline SP - 1979 EP - 95 JF - Toxins JO - Toxins (Basel) VL - 6 IS - 7 N2 - Taipans are highly venomous Australo-Papuan elapids. A new species of taipan, the Western Desert Taipan (Oxyuranus temporalis), has been discovered with two specimens housed in captivity at the Adelaide Zoo. This study is the first investigation of O. temporalis venom and seeks to characterise and compare the neurotoxicity, lethality and biochemical properties of O. temporalis venom with other taipan venoms. Analysis of O. temporalis venom using size-exclusion and reverse-phase HPLC indicated a markedly simplified "profile" compared to other taipan venoms. SDS-PAGE and agarose gel electrophoresis analysis also indicated a relatively simple composition. Murine LD50 studies showed that O. temporalis venom is less lethal than O. microlepidotus venom. Venoms were tested in vitro, using the chick biventer cervicis nerve-muscle preparation. Based on t90 values, O. temporalis venom is highly neurotoxic abolishing indirect twitches far more rapidly than other taipan venoms. O. temporalis venom also abolished responses to exogenous acetylcholine and carbachol, indicating the presence of postsynaptic neurotoxins. Prior administration of CSL Taipan antivenom (CSL Limited) neutralised the inhibitory effects of all taipan venoms. The results of this study suggest that the venom of the O. temporalis is highly neurotoxic in vitro and may contain procoagulant toxins, making this snake potentially dangerous to humans. SN - 2072-6651 UR - https://www.unboundmedicine.com/medline/citation/24992081/Comparative_studies_of_the_venom_of_a_new_Taipan_species_Oxyuranus_temporalis_with_other_members_of_its_genus_ L2 - http://www.mdpi.com/resolver?pii=toxins6071979 DB - PRIME DP - Unbound Medicine ER -