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Metabolic discrimination of Swertia mussotii and Swertia chirayita known as "Zangyinchen" in traditional Tibetan medicine by (1)H NMR-based metabolomics.
J Pharm Biomed Anal. 2014 Sep; 98:364-70.JP

Abstract

Swertia mussotii Franch. and Swertia chirayita Buch.-Ham. have been commonly used under the same name "Zangyinchen" for the treatment of liver and gallbladder diseases in traditional Tibetan medicine. Detailed characterization and comparison of the complete set of metabolites of these two species are critical for their objective identification and quality control. In this study, a rapid, simple and comprehensive (1)H NMR-based metabolomics method was first developed to differentiate the two species. A broad range of metabolites, including iridoid glycosides, xanthones, triterpenoids, flavonoids, carbohydrates, and amino acids, were identified. Statistical analysis showed evident differences between the two species, and the major markers responsible for the differences were screened. In addition, quantitative (1)H NMR method (qHNMR) was used for the target analysis of the discriminating metabolites. The results showed that S. mussotii had significantly higher contents of gentiopicrin, isoorientin, glucose, loganic acid, and choline, whereas S. chirayita exhibited higher levels of swertiamarin, oleanolic acid, valine, and fatty acids. These findings indicate that (1)H NMR-based metabolomics is a reliable and effective method for the metabolic profiling and discrimination of the two Swertia species, and can be used to verify the genuine origin of Zangyinchen.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Fan G
College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Luo WZ
Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.
Luo SH
College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Li Y
College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Meng XL
College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Zhou XD
Department of Medicinal Chemistry, College of Pharmacy, Third Military Medical University, Chongqing 400038, China. Electronic address: zhouxd188@163.com.
Zhang Y
College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. Electronic address: tcmzhangyi@aliyun.com.

MeSH

CholineFatty AcidsFlavonoidsGlucoseIridoid GlucosidesIridoid GlycosidesIridoidsLuteolinMagnetic Resonance SpectroscopyMedicine, Tibetan TraditionalMetabolomeMetabolomicsOleanolic AcidPyronesSwertiaTerpenesValineXanthones

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24992216

Citation

Fan, Gang, et al. "Metabolic Discrimination of Swertia Mussotii and Swertia Chirayita Known as "Zangyinchen" in Traditional Tibetan Medicine By (1)H NMR-based Metabolomics." Journal of Pharmaceutical and Biomedical Analysis, vol. 98, 2014, pp. 364-70.
Fan G, Luo WZ, Luo SH, et al. Metabolic discrimination of Swertia mussotii and Swertia chirayita known as "Zangyinchen" in traditional Tibetan medicine by (1)H NMR-based metabolomics. J Pharm Biomed Anal. 2014;98:364-70.
Fan, G., Luo, W. Z., Luo, S. H., Li, Y., Meng, X. L., Zhou, X. D., & Zhang, Y. (2014). Metabolic discrimination of Swertia mussotii and Swertia chirayita known as "Zangyinchen" in traditional Tibetan medicine by (1)H NMR-based metabolomics. Journal of Pharmaceutical and Biomedical Analysis, 98, 364-70. https://doi.org/10.1016/j.jpba.2014.06.014
Fan G, et al. Metabolic Discrimination of Swertia Mussotii and Swertia Chirayita Known as "Zangyinchen" in Traditional Tibetan Medicine By (1)H NMR-based Metabolomics. J Pharm Biomed Anal. 2014;98:364-70. PubMed PMID: 24992216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic discrimination of Swertia mussotii and Swertia chirayita known as "Zangyinchen" in traditional Tibetan medicine by (1)H NMR-based metabolomics. AU - Fan,Gang, AU - Luo,Wei-Zao, AU - Luo,Shang-Hua, AU - Li,Yan, AU - Meng,Xian-Li, AU - Zhou,Xiang-Dong, AU - Zhang,Yi, Y1 - 2014/06/16/ PY - 2014/02/18/received PY - 2014/06/08/revised PY - 2014/06/09/accepted PY - 2014/7/4/entrez PY - 2014/7/6/pubmed PY - 2015/4/22/medline KW - (1)H NMR KW - Metabolomics KW - Multivariate statistical analysis KW - Swertia chirayita KW - Swertia mussotii SP - 364 EP - 70 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 98 N2 - Swertia mussotii Franch. and Swertia chirayita Buch.-Ham. have been commonly used under the same name "Zangyinchen" for the treatment of liver and gallbladder diseases in traditional Tibetan medicine. Detailed characterization and comparison of the complete set of metabolites of these two species are critical for their objective identification and quality control. In this study, a rapid, simple and comprehensive (1)H NMR-based metabolomics method was first developed to differentiate the two species. A broad range of metabolites, including iridoid glycosides, xanthones, triterpenoids, flavonoids, carbohydrates, and amino acids, were identified. Statistical analysis showed evident differences between the two species, and the major markers responsible for the differences were screened. In addition, quantitative (1)H NMR method (qHNMR) was used for the target analysis of the discriminating metabolites. The results showed that S. mussotii had significantly higher contents of gentiopicrin, isoorientin, glucose, loganic acid, and choline, whereas S. chirayita exhibited higher levels of swertiamarin, oleanolic acid, valine, and fatty acids. These findings indicate that (1)H NMR-based metabolomics is a reliable and effective method for the metabolic profiling and discrimination of the two Swertia species, and can be used to verify the genuine origin of Zangyinchen. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/24992216/Metabolic_discrimination_of_Swertia_mussotii_and_Swertia_chirayita_known_as_"Zangyinchen"_in_traditional_Tibetan_medicine_by__1_H_NMR_based_metabolomics_ DB - PRIME DP - Unbound Medicine ER -