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The utility of p63, p40, and GATA-binding protein 3 immunohistochemistry in diagnosing micropapillary urothelial carcinoma.
Hum Pathol. 2014 Sep; 45(9):1824-9.HP

Abstract

Micropapillary urothelial carcinoma (MPUC) is an uncommon variant of urothelial carcinoma (UC) with an aggressive clinical course. There have been limited studies on the UC markers GATA-binding protein 3 (GATA3), p63, and p40 in MPUC. Our study investigated the immunoreactivity of these 3 markers in MPUC compared with conventional UC of different grades and stages. Immunohistochemistry was performed on 62 cases of high-grade urothelial carcinoma (HGUC), 16 low-grade urothelial carcinoma (LGUC), and 20 MPUC. p63 expression was strong and diffuse in all LGUC, significantly decreased in high stage and HGUC, and virtually absent in MPUC. p40 expression was decreased in HGUC and markedly decreased in MPUC relative to LGUC. These results suggest that loss of p63 expression in a UC appears to be associated with adverse features--including cases with micropapillary differentiation. Decreased GATA3 expression was seen frequently in high-grade and high-pathologic stage (≥pT2) tumors but was retained in MPUC cases. The findings of retained GATA3 expression in MPUC, which often shows a loss of expression of other urothelial markers such as p63, may be helpful for determining the origin of micropapillary carcinoma of unknown primary. Compared with the traditional markers p63 and p40, GATA3 is the most sensitive marker of conventional UC and MPUC.

Authors+Show Affiliations

Department of Pathology, Northwestern Memorial Hospital, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.Department of Pathology, Northwestern Memorial Hospital, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.Department of Pathology, Northwestern Memorial Hospital, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.Department of Pathology, Westchester Medical Center, Valhalla, NY 10595.Department of Urology, Northwestern Memorial Hospital, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.Department of Pathology, Northwestern Memorial Hospital, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.Department of Pathology, Northwestern Memorial Hospital, Northwestern University Feinberg School of Medicine, Chicago, IL 60611. Electronic address: xyang@northwestern.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24993315

Citation

Lin, Xiaoqi, et al. "The Utility of P63, P40, and GATA-binding Protein 3 Immunohistochemistry in Diagnosing Micropapillary Urothelial Carcinoma." Human Pathology, vol. 45, no. 9, 2014, pp. 1824-9.
Lin X, Zhu B, Villa C, et al. The utility of p63, p40, and GATA-binding protein 3 immunohistochemistry in diagnosing micropapillary urothelial carcinoma. Hum Pathol. 2014;45(9):1824-9.
Lin, X., Zhu, B., Villa, C., Zhong, M., Kundu, S., Rohan, S. M., & Yang, X. J. (2014). The utility of p63, p40, and GATA-binding protein 3 immunohistochemistry in diagnosing micropapillary urothelial carcinoma. Human Pathology, 45(9), 1824-9. https://doi.org/10.1016/j.humpath.2014.04.015
Lin X, et al. The Utility of P63, P40, and GATA-binding Protein 3 Immunohistochemistry in Diagnosing Micropapillary Urothelial Carcinoma. Hum Pathol. 2014;45(9):1824-9. PubMed PMID: 24993315.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The utility of p63, p40, and GATA-binding protein 3 immunohistochemistry in diagnosing micropapillary urothelial carcinoma. AU - Lin,Xiaoqi, AU - Zhu,Bing, AU - Villa,Celina, AU - Zhong,Minghao, AU - Kundu,Shilajit, AU - Rohan,Stephen M, AU - Yang,Ximing J, Y1 - 2014/05/08/ PY - 2014/01/23/received PY - 2014/04/13/revised PY - 2014/04/16/accepted PY - 2014/7/5/entrez PY - 2014/7/6/pubmed PY - 2014/11/13/medline KW - GATA3 KW - Micropapillary KW - Urothelial carcinoma KW - p40 KW - p63 SP - 1824 EP - 9 JF - Human pathology JO - Hum. Pathol. VL - 45 IS - 9 N2 - Micropapillary urothelial carcinoma (MPUC) is an uncommon variant of urothelial carcinoma (UC) with an aggressive clinical course. There have been limited studies on the UC markers GATA-binding protein 3 (GATA3), p63, and p40 in MPUC. Our study investigated the immunoreactivity of these 3 markers in MPUC compared with conventional UC of different grades and stages. Immunohistochemistry was performed on 62 cases of high-grade urothelial carcinoma (HGUC), 16 low-grade urothelial carcinoma (LGUC), and 20 MPUC. p63 expression was strong and diffuse in all LGUC, significantly decreased in high stage and HGUC, and virtually absent in MPUC. p40 expression was decreased in HGUC and markedly decreased in MPUC relative to LGUC. These results suggest that loss of p63 expression in a UC appears to be associated with adverse features--including cases with micropapillary differentiation. Decreased GATA3 expression was seen frequently in high-grade and high-pathologic stage (≥pT2) tumors but was retained in MPUC cases. The findings of retained GATA3 expression in MPUC, which often shows a loss of expression of other urothelial markers such as p63, may be helpful for determining the origin of micropapillary carcinoma of unknown primary. Compared with the traditional markers p63 and p40, GATA3 is the most sensitive marker of conventional UC and MPUC. SN - 1532-8392 UR - https://www.unboundmedicine.com/medline/citation/24993315/The_utility_of_p63_p40_and_GATA_binding_protein_3_immunohistochemistry_in_diagnosing_micropapillary_urothelial_carcinoma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0046-8177(14)00187-7 DB - PRIME DP - Unbound Medicine ER -