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Impact of oral fluid collection device on cannabinoid stability following smoked cannabis.
Drug Test Anal 2015; 7(2):114-20DT

Abstract

Evaluation of cannabinoid stability in authentic oral fluid (OF) is critical, as most OF stability studies employed fortified or synthetic OF. Participants (n = 16) smoked a 6.8% delta-9-tetrahydrocannabinol (THC) cigarette, and baseline concentrations of THC, 11-nor-9-carboxy-THC (THCCOOH), cannabidiol (CBD), and cannabinol (CBN) were determined within 24 h in 16 separate pooled samples (collected 1 h before to 10.5 or 13 h after smoking). OF was collected with the StatSure Saliva Sampler™ and Oral-Eze® devices. Oral-Eze samples were re-analyzed after room temperature (RT) storage for 1 week, and for both devices after 4 °C for 1 and 4 weeks, and -20 °C for 4 and 24 weeks. Concentrations ±20% from initial concentrations were considered stable. With the StatSure device, all cannabinoids were within 80-120% median %baseline for all storage conditions. Individual THC, CBD, CBN and THCCOOH pool concentrations were stable in 100%, 100%, 80-94% and >85%, respectively, across storage conditions. With the Oral-Eze device, at RT or refrigerated storage (for 1 and 4 weeks), THC, CBD and THCCOOH were stable in 94-100%, 78-89%, and 93-100% of samples, respectively, while CBN concentrations were 53-79% stable. However, after 24 weeks at -20 °C, stability decreased, especially for CBD, with a median of 56% stability. Overall, the collection devices' elution/stabilizing buffers provided good stability for OF cannabinoids, with the exception of the more labile CBN. To ensure OF cannabinoid concentration accuracy, these data suggest analysis within 4 weeks at 4 °C storage for Oral-Eze collection and within 4 weeks at 4 °C or 24 weeks at -20 °C for StatSure collection. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.

Authors+Show Affiliations

Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, 251 Bayview Blvd, Suite 200, Baltimore, MD, 21224, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24995604

Citation

Anizan, Sébastien, et al. "Impact of Oral Fluid Collection Device On Cannabinoid Stability Following Smoked Cannabis." Drug Testing and Analysis, vol. 7, no. 2, 2015, pp. 114-20.
Anizan S, Bergamaschi MM, Barnes AJ, et al. Impact of oral fluid collection device on cannabinoid stability following smoked cannabis. Drug Test Anal. 2015;7(2):114-20.
Anizan, S., Bergamaschi, M. M., Barnes, A. J., Milman, G., Desrosiers, N., Lee, D., ... Huestis, M. A. (2015). Impact of oral fluid collection device on cannabinoid stability following smoked cannabis. Drug Testing and Analysis, 7(2), pp. 114-20. doi:10.1002/dta.1688.
Anizan S, et al. Impact of Oral Fluid Collection Device On Cannabinoid Stability Following Smoked Cannabis. Drug Test Anal. 2015;7(2):114-20. PubMed PMID: 24995604.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of oral fluid collection device on cannabinoid stability following smoked cannabis. AU - Anizan,Sébastien, AU - Bergamaschi,Mateus M, AU - Barnes,Allan J, AU - Milman,Garry, AU - Desrosiers,Nathalie, AU - Lee,Dayong, AU - Gorelick,David A, AU - Huestis,Marilyn A, Y1 - 2014/07/03/ PY - 2014/05/06/received PY - 2014/06/06/revised PY - 2014/06/08/accepted PY - 2014/7/5/entrez PY - 2014/7/6/pubmed PY - 2015/9/25/medline KW - THC KW - cannabinoids KW - cannabis KW - oral fluid KW - stability SP - 114 EP - 20 JF - Drug testing and analysis JO - Drug Test Anal VL - 7 IS - 2 N2 - Evaluation of cannabinoid stability in authentic oral fluid (OF) is critical, as most OF stability studies employed fortified or synthetic OF. Participants (n = 16) smoked a 6.8% delta-9-tetrahydrocannabinol (THC) cigarette, and baseline concentrations of THC, 11-nor-9-carboxy-THC (THCCOOH), cannabidiol (CBD), and cannabinol (CBN) were determined within 24 h in 16 separate pooled samples (collected 1 h before to 10.5 or 13 h after smoking). OF was collected with the StatSure Saliva Sampler™ and Oral-Eze® devices. Oral-Eze samples were re-analyzed after room temperature (RT) storage for 1 week, and for both devices after 4 °C for 1 and 4 weeks, and -20 °C for 4 and 24 weeks. Concentrations ±20% from initial concentrations were considered stable. With the StatSure device, all cannabinoids were within 80-120% median %baseline for all storage conditions. Individual THC, CBD, CBN and THCCOOH pool concentrations were stable in 100%, 100%, 80-94% and >85%, respectively, across storage conditions. With the Oral-Eze device, at RT or refrigerated storage (for 1 and 4 weeks), THC, CBD and THCCOOH were stable in 94-100%, 78-89%, and 93-100% of samples, respectively, while CBN concentrations were 53-79% stable. However, after 24 weeks at -20 °C, stability decreased, especially for CBD, with a median of 56% stability. Overall, the collection devices' elution/stabilizing buffers provided good stability for OF cannabinoids, with the exception of the more labile CBN. To ensure OF cannabinoid concentration accuracy, these data suggest analysis within 4 weeks at 4 °C storage for Oral-Eze collection and within 4 weeks at 4 °C or 24 weeks at -20 °C for StatSure collection. Published 2014. This article is a U.S. Government work and is in the public domain in the USA. SN - 1942-7611 UR - https://www.unboundmedicine.com/medline/citation/24995604/Impact_of_oral_fluid_collection_device_on_cannabinoid_stability_following_smoked_cannabis_ L2 - https://doi.org/10.1002/dta.1688 DB - PRIME DP - Unbound Medicine ER -