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Exogenous glucagon-like peptide-2 improves outcomes of intestinal adaptation in a distal-intestinal resection neonatal piglet model of short bowel syndrome.
Pediatr Res. 2014 Oct; 76(4):370-7.PR

Abstract

BACKGROUND

Endogenous glucagon-like peptide-2 (GLP-2) levels and intestinal adaptation are reduced in distal-intestinal resection animal models of short bowel syndrome (SBS) that lack remnant ileum. We hypothesized that exogenous GLP-2 would improve intestinal adaptation in a distal-intestinal resection neonatal piglet model of SBS.

METHODS

In all, 35 piglets were randomized to 2 treatment and 3 surgical groups: control (sham), 75% mid-intestinal resection (JI), and 75% distal-intestinal resection (JC). Parenteral nutrition (PN) commenced on day 1 and was weaned as enteral nutrition (EN) advanced. IV GLP-2 (11 nmol/kg/d) or saline was initiated on day 2. Piglets were maintained for 14 d. Clinical, functional, morphological, and histological outcomes were obtained.

RESULTS

JC-GLP-2 piglets had fewer days on PN (10.0 ± 0.6 vs. 13.8 ± 0.2), more days on EN (4.0 ± 0.6 vs. 0.2 ± 0.2), a higher percentage of EN at termination (92 ± 5 vs. 52 ± 10%), fewer days of diarrhea (8.0 ± 0.7 vs. 12.3 ± 0.4), increased intestinal length (19 ± 4 vs. -5 ± 3%), and deeper jejunal crypts (248 ± 21 vs. 172 ± 12 μm), compared with saline piglets.

CONCLUSION

GLP-2 therapy improves clinical, morphological, and histological outcomes of intestinal adaptation in a distal-intestinal resection model of SBS. Since this anatomical subtype represents the majority of clinical cases of neonatal SBS, these results support a potential role for GLP-2 therapy in pediatric SBS.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Suri M
Department of General Surgery, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.
Turner JM
Department of Paediatrics, University of Alberta, Edmonton, Alberta, Canada.
Sigalet DL
Department of General Surgery, University of Calgary, Calgary, Alberta, Canada.
Wizzard PR
Department of Paediatrics, University of Alberta, Edmonton, Alberta, Canada.
Nation PN
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada.
Ball RO
Department of Agricultural Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
Pencharz PB
1] Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada [2] Department of Paediatrics, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada [3] Department of Nutritional Sciences, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.
Brubaker PL
1] Department of Physiology, University of Toronto, Toronto, Ontario, Canada [2] Department of Medicine, University of Toronto, Toronto, Ontario, Canada.
Wales PW
1] Department of General Surgery, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada [2] Department of Paediatrics, University of Alberta, Edmonton, Alberta, Canada [3] Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.

MeSH

Adaptation, PhysiologicalAnimalsAnimals, NewbornDisease Models, AnimalGlucagon-Like Peptide 2Intestine, SmallRNA, MessengerShort Bowel SyndromeSwine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24995913

Citation

Suri, Megha, et al. "Exogenous Glucagon-like Peptide-2 Improves Outcomes of Intestinal Adaptation in a Distal-intestinal Resection Neonatal Piglet Model of Short Bowel Syndrome." Pediatric Research, vol. 76, no. 4, 2014, pp. 370-7.
Suri M, Turner JM, Sigalet DL, et al. Exogenous glucagon-like peptide-2 improves outcomes of intestinal adaptation in a distal-intestinal resection neonatal piglet model of short bowel syndrome. Pediatr Res. 2014;76(4):370-7.
Suri, M., Turner, J. M., Sigalet, D. L., Wizzard, P. R., Nation, P. N., Ball, R. O., Pencharz, P. B., Brubaker, P. L., & Wales, P. W. (2014). Exogenous glucagon-like peptide-2 improves outcomes of intestinal adaptation in a distal-intestinal resection neonatal piglet model of short bowel syndrome. Pediatric Research, 76(4), 370-7. https://doi.org/10.1038/pr.2014.97
Suri M, et al. Exogenous Glucagon-like Peptide-2 Improves Outcomes of Intestinal Adaptation in a Distal-intestinal Resection Neonatal Piglet Model of Short Bowel Syndrome. Pediatr Res. 2014;76(4):370-7. PubMed PMID: 24995913.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exogenous glucagon-like peptide-2 improves outcomes of intestinal adaptation in a distal-intestinal resection neonatal piglet model of short bowel syndrome. AU - Suri,Megha, AU - Turner,Justine M, AU - Sigalet,David L, AU - Wizzard,Pamela R, AU - Nation,Patrick N, AU - Ball,Ron O, AU - Pencharz,Paul B, AU - Brubaker,Patricia L, AU - Wales,Paul W, Y1 - 2014/07/04/ PY - 2013/12/15/received PY - 2014/04/02/accepted PY - 2014/7/5/entrez PY - 2014/7/6/pubmed PY - 2015/5/27/medline SP - 370 EP - 7 JF - Pediatric research JO - Pediatr Res VL - 76 IS - 4 N2 - BACKGROUND: Endogenous glucagon-like peptide-2 (GLP-2) levels and intestinal adaptation are reduced in distal-intestinal resection animal models of short bowel syndrome (SBS) that lack remnant ileum. We hypothesized that exogenous GLP-2 would improve intestinal adaptation in a distal-intestinal resection neonatal piglet model of SBS. METHODS: In all, 35 piglets were randomized to 2 treatment and 3 surgical groups: control (sham), 75% mid-intestinal resection (JI), and 75% distal-intestinal resection (JC). Parenteral nutrition (PN) commenced on day 1 and was weaned as enteral nutrition (EN) advanced. IV GLP-2 (11 nmol/kg/d) or saline was initiated on day 2. Piglets were maintained for 14 d. Clinical, functional, morphological, and histological outcomes were obtained. RESULTS: JC-GLP-2 piglets had fewer days on PN (10.0 ± 0.6 vs. 13.8 ± 0.2), more days on EN (4.0 ± 0.6 vs. 0.2 ± 0.2), a higher percentage of EN at termination (92 ± 5 vs. 52 ± 10%), fewer days of diarrhea (8.0 ± 0.7 vs. 12.3 ± 0.4), increased intestinal length (19 ± 4 vs. -5 ± 3%), and deeper jejunal crypts (248 ± 21 vs. 172 ± 12 μm), compared with saline piglets. CONCLUSION: GLP-2 therapy improves clinical, morphological, and histological outcomes of intestinal adaptation in a distal-intestinal resection model of SBS. Since this anatomical subtype represents the majority of clinical cases of neonatal SBS, these results support a potential role for GLP-2 therapy in pediatric SBS. SN - 1530-0447 UR - https://www.unboundmedicine.com/medline/citation/24995913/Exogenous_glucagon_like_peptide_2_improves_outcomes_of_intestinal_adaptation_in_a_distal_intestinal_resection_neonatal_piglet_model_of_short_bowel_syndrome_ DB - PRIME DP - Unbound Medicine ER -