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Atomic force microscopy (AFM) imaging suggests that stromal interaction molecule 1 (STIM1) binds to Orai1 with sixfold symmetry.
FEBS Lett. 2014 Aug 25; 588(17):2874-80.FL

Abstract

Depletion of Ca(2+) from the endoplasmic reticulum (ER) lumen triggers the opening of Ca(2+) release-activated Ca(2+) (CRAC) channels at the plasma membrane. CRAC channels are activated by stromal interaction molecule 1 (STIM1), an ER resident protein that senses Ca(2+) store depletion and interacts with Orai1, the pore-forming subunit of the channel. The subunit stoichiometry of the CRAC channel is controversial. Here we provide evidence, using atomic force microscopy (AFM) imaging, that Orai1 assembles as a hexamer, and that STIM1 binds to Orai1 with sixfold symmetry. STIM1 associates with Orai1 in the form of monomers, dimers, and multimeric string-like structures that form links between the Orai1 hexamers. Our results provide new insights into the nature of the interactions between STIM1 and Orai1.

Authors+Show Affiliations

Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, United Kingdom.Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, United Kingdom.Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, United Kingdom.Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, United Kingdom. Electronic address: jme1000@cam.ac.uk.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24996186

Citation

Balasuriya, Dilshan, et al. "Atomic Force Microscopy (AFM) Imaging Suggests That Stromal Interaction Molecule 1 (STIM1) Binds to Orai1 With Sixfold Symmetry." FEBS Letters, vol. 588, no. 17, 2014, pp. 2874-80.
Balasuriya D, Srivats S, Murrell-Lagnado RD, et al. Atomic force microscopy (AFM) imaging suggests that stromal interaction molecule 1 (STIM1) binds to Orai1 with sixfold symmetry. FEBS Lett. 2014;588(17):2874-80.
Balasuriya, D., Srivats, S., Murrell-Lagnado, R. D., & Edwardson, J. M. (2014). Atomic force microscopy (AFM) imaging suggests that stromal interaction molecule 1 (STIM1) binds to Orai1 with sixfold symmetry. FEBS Letters, 588(17), 2874-80. https://doi.org/10.1016/j.febslet.2014.06.054
Balasuriya D, et al. Atomic Force Microscopy (AFM) Imaging Suggests That Stromal Interaction Molecule 1 (STIM1) Binds to Orai1 With Sixfold Symmetry. FEBS Lett. 2014 Aug 25;588(17):2874-80. PubMed PMID: 24996186.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Atomic force microscopy (AFM) imaging suggests that stromal interaction molecule 1 (STIM1) binds to Orai1 with sixfold symmetry. AU - Balasuriya,Dilshan, AU - Srivats,Shyam, AU - Murrell-Lagnado,Ruth D, AU - Edwardson,J Michael, Y1 - 2014/07/01/ PY - 2014/04/24/received PY - 2014/06/19/revised PY - 2014/06/23/accepted PY - 2014/7/5/entrez PY - 2014/7/6/pubmed PY - 2014/10/16/medline KW - Atomic force microscopy KW - CRAC channel KW - Orai1 KW - STIM1 KW - Store-operated Ca(2+) entry SP - 2874 EP - 80 JF - FEBS letters JO - FEBS Lett. VL - 588 IS - 17 N2 - Depletion of Ca(2+) from the endoplasmic reticulum (ER) lumen triggers the opening of Ca(2+) release-activated Ca(2+) (CRAC) channels at the plasma membrane. CRAC channels are activated by stromal interaction molecule 1 (STIM1), an ER resident protein that senses Ca(2+) store depletion and interacts with Orai1, the pore-forming subunit of the channel. The subunit stoichiometry of the CRAC channel is controversial. Here we provide evidence, using atomic force microscopy (AFM) imaging, that Orai1 assembles as a hexamer, and that STIM1 binds to Orai1 with sixfold symmetry. STIM1 associates with Orai1 in the form of monomers, dimers, and multimeric string-like structures that form links between the Orai1 hexamers. Our results provide new insights into the nature of the interactions between STIM1 and Orai1. SN - 1873-3468 UR - https://www.unboundmedicine.com/medline/citation/24996186/Atomic_force_microscopy__AFM__imaging_suggests_that_stromal_interaction_molecule_1__STIM1__binds_to_Orai1_with_sixfold_symmetry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-5793(14)00518-3 DB - PRIME DP - Unbound Medicine ER -