TY - JOUR T1 - Comparative chemical and biochemical analysis of extracts of Hibiscus sabdariffa. AU - Sindi,Heba A, AU - Marshall,Lisa J, AU - Morgan,Michael R A, Y1 - 2014/05/06/ PY - 2013/11/19/received PY - 2014/04/25/revised PY - 2014/04/27/accepted PY - 2014/7/6/entrez PY - 2014/7/6/pubmed PY - 2015/12/15/medline KW - Anthocyanins KW - Antioxidant capacity KW - Cyanidin 3-sambubioside KW - DPPH KW - Delphinidin 3-sambubioside KW - FRAP KW - Hibiscus sabdariffa KW - Solvent extraction KW - TEAC KW - Total polyphenols SP - 23 EP - 9 JF - Food chemistry JO - Food Chem VL - 164 N2 - Hibiscus sabdariffa extracts have attracted attention because of potentially useful bioactivity. However, there have been no systematic studies of extraction efficiencies of H. sabdariffa. The nature of extracts used in different studies has varied considerably, making comparisons difficult. Therefore, a systematic study of extracts of H. sabdariffa made with different solvents was carried out using water, methanol, ethyl acetate and hexane in the presence/absence of formic acid, using different extraction times and temperatures. The extracts were analysed for total polyphenol content, antioxidant capacity using DPPH, FRAP and TEAC assays, and specific anthocyanins were determined using HPLC and LC-MS. The results showed the highest antioxidant capacities were obtained by extracting using water, with or without formic acid, for 10 min at 100°C. These extracts provided the highest concentrations of cyanidin 3-sambubioside and delphinidin 3-sambubioside. It will be important to use extraction conditions giving optimal extraction efficiencies for subsequent bioactivity experiments. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/24996300/Comparative_chemical_and_biochemical_analysis_of_extracts_of_Hibiscus_sabdariffa_ DB - PRIME DP - Unbound Medicine ER -