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Development and validation of an HPLC-method for determination of free and bound phenolic acids in cereals after solid-phase extraction.
Food Chem. 2012 Oct 01; 134(3):1624-32.FC

Abstract

Whole cereal grains are a good source of phenolic acids associated with reduced risk of chronic diseases. This paper reports the development and validation of a high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the determination of phenolic acids in cereals in either free or bound form. Extraction of free phenolic acids and clean-up was performed by an optimised solid-phase extraction (SPE) protocol on Oasis HLB cartridges using aqueous methanol as eluant. The mean recovery of analytes ranged between 84% and 106%. Bound phenolic acids were extracted using alkaline hydrolysis with mean recoveries of 80-95%, except for gallic acid, caffeic acid and protocatechuic acid. Both free and bound phenolic extracts were separated on a Nucleosil 100 C18 column, 5 μm (250 mm × 4.6 mm) thermostated at 30 °C, using a linear gradient elution system consisting of 1% (v/v) acetic acid in methanol. Method validation was performed by means of linearity, accuracy, intra-day and inter-day precision and sensitivity. Detection limits ranged between 0.13 and 0.18 μg/g. The method was applied to the analysis of free and bound phenolic acids contents in durum wheat, bread wheat, barley, oat, rice, rye, corn and triticale.

Authors+Show Affiliations

Cereal Institute, National Agricultural Research Foundation, P.O. Box 60411, Thessaloniki 57 001, Greece.Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University, Thessaloniki 54 124, Greece.Laboratory of Food Chemistry and Biochemistry, Department of Food Science and Technology, Aristotle University, P.O. Box 235, Thessaloniki 54 124, Greece.Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University, Thessaloniki 54 124, Greece.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25005991

Citation

Irakli, Maria N., et al. "Development and Validation of an HPLC-method for Determination of Free and Bound Phenolic Acids in Cereals After Solid-phase Extraction." Food Chemistry, vol. 134, no. 3, 2012, pp. 1624-32.
Irakli MN, Samanidou VF, Biliaderis CG, et al. Development and validation of an HPLC-method for determination of free and bound phenolic acids in cereals after solid-phase extraction. Food Chem. 2012;134(3):1624-32.
Irakli, M. N., Samanidou, V. F., Biliaderis, C. G., & Papadoyannis, I. N. (2012). Development and validation of an HPLC-method for determination of free and bound phenolic acids in cereals after solid-phase extraction. Food Chemistry, 134(3), 1624-32. https://doi.org/10.1016/j.foodchem.2012.03.046
Irakli MN, et al. Development and Validation of an HPLC-method for Determination of Free and Bound Phenolic Acids in Cereals After Solid-phase Extraction. Food Chem. 2012 Oct 1;134(3):1624-32. PubMed PMID: 25005991.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of an HPLC-method for determination of free and bound phenolic acids in cereals after solid-phase extraction. AU - Irakli,Maria N, AU - Samanidou,Victoria F, AU - Biliaderis,Costas G, AU - Papadoyannis,Ioannis N, Y1 - 2012/03/19/ PY - 2011/11/07/received PY - 2012/02/29/revised PY - 2012/03/11/accepted PY - 2014/7/10/entrez PY - 2012/10/1/pubmed PY - 2017/4/19/medline SP - 1624 EP - 32 JF - Food chemistry JO - Food Chem VL - 134 IS - 3 N2 - Whole cereal grains are a good source of phenolic acids associated with reduced risk of chronic diseases. This paper reports the development and validation of a high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the determination of phenolic acids in cereals in either free or bound form. Extraction of free phenolic acids and clean-up was performed by an optimised solid-phase extraction (SPE) protocol on Oasis HLB cartridges using aqueous methanol as eluant. The mean recovery of analytes ranged between 84% and 106%. Bound phenolic acids were extracted using alkaline hydrolysis with mean recoveries of 80-95%, except for gallic acid, caffeic acid and protocatechuic acid. Both free and bound phenolic extracts were separated on a Nucleosil 100 C18 column, 5 μm (250 mm × 4.6 mm) thermostated at 30 °C, using a linear gradient elution system consisting of 1% (v/v) acetic acid in methanol. Method validation was performed by means of linearity, accuracy, intra-day and inter-day precision and sensitivity. Detection limits ranged between 0.13 and 0.18 μg/g. The method was applied to the analysis of free and bound phenolic acids contents in durum wheat, bread wheat, barley, oat, rice, rye, corn and triticale. SN - 0308-8146 UR - https://www.unboundmedicine.com/medline/citation/25005991/Development_and_validation_of_an_HPLC_method_for_determination_of_free_and_bound_phenolic_acids_in_cereals_after_solid_phase_extraction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(12)00509-2 DB - PRIME DP - Unbound Medicine ER -