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Role of Gag and lipids during HIV-1 assembly in CD4(+) T cells and macrophages.
Front Microbiol. 2014; 5:312.FM

Abstract

HIV-1 is an RNA enveloped virus that preferentially infects CD4(+) T lymphocytes and also macrophages. In CD4(+) T cells, HIV-1 mainly buds from the host cell plasma membrane. The viral Gag polyprotein targets the plasma membrane and is the orchestrator of the HIV assembly as its expression is sufficient to promote the formation of virus-like particles carrying a lipidic envelope derived from the host cell membrane. Certain lipids are enriched in the viral membrane and are thought to play a key role in the assembly process and the envelop composition. A large body of work performed on infected CD4(+) T cells has provided important knowledge about the assembly process and the membrane virus lipid composition. While HIV assembly and budding in macrophages is thought to follow the same general Gag-driven mechanism as in T-lymphocytes, the HIV cycle in macrophage exhibits specific features. In these cells, new virions bud from the limiting membrane of seemingly intracellular compartments, where they accumulate while remaining infectious. These structures are now often referred to as Virus Containing Compartments (VCCs). Recent studies suggest that VCCs represent intracellularly sequestered regions of the plasma membrane, but their precise nature remains elusive. The proteomic and lipidomic characterization of virions produced by T cells or macrophages has highlighted the similarity between their composition and that of the plasma membrane of producer cells, as well as their enrichment in acidic lipids, some components of raft lipids and in tetraspanin-enriched microdomains. It is likely that Gag promotes the coalescence of these components into an assembly platform from which viral budding takes place. How Gag exactly interacts with membrane lipids and what are the mechanisms involved in the interaction between the different membrane nanodomains within the assembly platform remains unclear. Here we review recent literature regarding the role of Gag and lipids on HIV-1 assembly in CD4(+) T cells and macrophages.

Authors+Show Affiliations

Membrane Domains and Viral Assembly, CNRS UMR-5236, Centre d'étude d'agents Pathogènes et Biotechnologies pour la Santé Montpellier, Cedex, France.Intracellular Transport and Immunity, Immunité et Cancer, Institut Curie - Inserm U932 Paris, France.Membrane Domains and Viral Assembly, CNRS UMR-5236, Centre d'étude d'agents Pathogènes et Biotechnologies pour la Santé Montpellier, Cedex, France.Intracellular Transport and Immunity, Immunité et Cancer, Institut Curie - Inserm U932 Paris, France.Membrane Domains and Viral Assembly, CNRS UMR-5236, Centre d'étude d'agents Pathogènes et Biotechnologies pour la Santé Montpellier, Cedex, France.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

25009540

Citation

Mariani, Charlotte, et al. "Role of Gag and Lipids During HIV-1 Assembly in CD4(+) T Cells and Macrophages." Frontiers in Microbiology, vol. 5, 2014, p. 312.
Mariani C, Desdouits M, Favard C, et al. Role of Gag and lipids during HIV-1 assembly in CD4(+) T cells and macrophages. Front Microbiol. 2014;5:312.
Mariani, C., Desdouits, M., Favard, C., Benaroch, P., & Muriaux, D. M. (2014). Role of Gag and lipids during HIV-1 assembly in CD4(+) T cells and macrophages. Frontiers in Microbiology, 5, 312. https://doi.org/10.3389/fmicb.2014.00312
Mariani C, et al. Role of Gag and Lipids During HIV-1 Assembly in CD4(+) T Cells and Macrophages. Front Microbiol. 2014;5:312. PubMed PMID: 25009540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of Gag and lipids during HIV-1 assembly in CD4(+) T cells and macrophages. AU - Mariani,Charlotte, AU - Desdouits,Marion, AU - Favard,Cyril, AU - Benaroch,Philippe, AU - Muriaux,Delphine M, Y1 - 2014/06/25/ PY - 2014/03/19/received PY - 2014/06/08/accepted PY - 2014/7/11/entrez PY - 2014/7/11/pubmed PY - 2014/7/11/medline KW - CD4+ T cells KW - Gag KW - HIV-1 KW - assembly KW - lipids KW - macrophages SP - 312 EP - 312 JF - Frontiers in microbiology JO - Front Microbiol VL - 5 N2 - HIV-1 is an RNA enveloped virus that preferentially infects CD4(+) T lymphocytes and also macrophages. In CD4(+) T cells, HIV-1 mainly buds from the host cell plasma membrane. The viral Gag polyprotein targets the plasma membrane and is the orchestrator of the HIV assembly as its expression is sufficient to promote the formation of virus-like particles carrying a lipidic envelope derived from the host cell membrane. Certain lipids are enriched in the viral membrane and are thought to play a key role in the assembly process and the envelop composition. A large body of work performed on infected CD4(+) T cells has provided important knowledge about the assembly process and the membrane virus lipid composition. While HIV assembly and budding in macrophages is thought to follow the same general Gag-driven mechanism as in T-lymphocytes, the HIV cycle in macrophage exhibits specific features. In these cells, new virions bud from the limiting membrane of seemingly intracellular compartments, where they accumulate while remaining infectious. These structures are now often referred to as Virus Containing Compartments (VCCs). Recent studies suggest that VCCs represent intracellularly sequestered regions of the plasma membrane, but their precise nature remains elusive. The proteomic and lipidomic characterization of virions produced by T cells or macrophages has highlighted the similarity between their composition and that of the plasma membrane of producer cells, as well as their enrichment in acidic lipids, some components of raft lipids and in tetraspanin-enriched microdomains. It is likely that Gag promotes the coalescence of these components into an assembly platform from which viral budding takes place. How Gag exactly interacts with membrane lipids and what are the mechanisms involved in the interaction between the different membrane nanodomains within the assembly platform remains unclear. Here we review recent literature regarding the role of Gag and lipids on HIV-1 assembly in CD4(+) T cells and macrophages. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/25009540/Role_of_Gag_and_lipids_during_HIV_1_assembly_in_CD4_+__T_cells_and_macrophages_ L2 - https://doi.org/10.3389/fmicb.2014.00312 DB - PRIME DP - Unbound Medicine ER -
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