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Integrated assessment of oxidative stress and DNA damage in earthworms (Eisenia fetida) exposed to azoxystrobin.
Ecotoxicol Environ Saf. 2014 Sep; 107:214-9.EE

Abstract

Azoxystrobin has been widely used in recent years. The present study investigated the oxidative stress and DNA damage effects of azoxystrobin on earthworms (Eisenia fetida). Earthworms were exposed to different azoxystrobin concentrations in an artificial soil (0, 0.1, 1, and 10mg/kg) and sampled on days 7, 14, 21, and 28. Superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POD), glutathione-S-transferase (GST), reactive oxygen species (ROS), and malondialdehyde (MDA) content were measured by an ultraviolet spectrophotometer to determine the antioxidant responses and lipid peroxidation. Single cell gel electrophoresis (SCGE) was used to detect DNA damage in the coelomocytes. Compared with these in the controls, earthworms exposed to azoxystrobin had excess ROS accumulation and greater SOD, POD, and GST activity while the opposite trend occurred for CAT activity. MDA content increased after 14-day exposure, and DNA damage was enhanced with an increase in the concentration of azoxystrobin. In conclusion, azoxystrobin caused oxidative stress leading to lipid peroxidation and DNA damage in earthworms.

Authors+Show Affiliations

National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agriculture University, 61 Daizong Road, Taian 271018, PR China. Electronic address: hyn_call@163.com.National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agriculture University, 61 Daizong Road, Taian 271018, PR China. Electronic address: lushzhu@vip.163.com.National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agriculture University, 61 Daizong Road, Taian 271018, PR China. Electronic address: wjh@sdau.edu.cn.National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agriculture University, 61 Daizong Road, Taian 271018, PR China. Electronic address: jwang@sdau.edu.cn.National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agriculture University, 61 Daizong Road, Taian 271018, PR China. Electronic address: huixie@sdau.edu.cn.National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agriculture University, 61 Daizong Road, Taian 271018, PR China. Electronic address: zsm1126@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25011117

Citation

Han, Yingnan, et al. "Integrated Assessment of Oxidative Stress and DNA Damage in Earthworms (Eisenia Fetida) Exposed to Azoxystrobin." Ecotoxicology and Environmental Safety, vol. 107, 2014, pp. 214-9.
Han Y, Zhu L, Wang J, et al. Integrated assessment of oxidative stress and DNA damage in earthworms (Eisenia fetida) exposed to azoxystrobin. Ecotoxicol Environ Saf. 2014;107:214-9.
Han, Y., Zhu, L., Wang, J., Wang, J., Xie, H., & Zhang, S. (2014). Integrated assessment of oxidative stress and DNA damage in earthworms (Eisenia fetida) exposed to azoxystrobin. Ecotoxicology and Environmental Safety, 107, 214-9. https://doi.org/10.1016/j.ecoenv.2014.06.006
Han Y, et al. Integrated Assessment of Oxidative Stress and DNA Damage in Earthworms (Eisenia Fetida) Exposed to Azoxystrobin. Ecotoxicol Environ Saf. 2014;107:214-9. PubMed PMID: 25011117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Integrated assessment of oxidative stress and DNA damage in earthworms (Eisenia fetida) exposed to azoxystrobin. AU - Han,Yingnan, AU - Zhu,Lusheng, AU - Wang,Jinhua, AU - Wang,Jun, AU - Xie,Hui, AU - Zhang,Shumin, Y1 - 2014/07/08/ PY - 2014/03/20/received PY - 2014/06/03/revised PY - 2014/06/05/accepted PY - 2014/7/11/entrez PY - 2014/7/11/pubmed PY - 2014/12/15/medline KW - Antioxidative system KW - GST KW - MDA KW - ROS KW - SCGE KW - Toxicity SP - 214 EP - 9 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 107 N2 - Azoxystrobin has been widely used in recent years. The present study investigated the oxidative stress and DNA damage effects of azoxystrobin on earthworms (Eisenia fetida). Earthworms were exposed to different azoxystrobin concentrations in an artificial soil (0, 0.1, 1, and 10mg/kg) and sampled on days 7, 14, 21, and 28. Superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POD), glutathione-S-transferase (GST), reactive oxygen species (ROS), and malondialdehyde (MDA) content were measured by an ultraviolet spectrophotometer to determine the antioxidant responses and lipid peroxidation. Single cell gel electrophoresis (SCGE) was used to detect DNA damage in the coelomocytes. Compared with these in the controls, earthworms exposed to azoxystrobin had excess ROS accumulation and greater SOD, POD, and GST activity while the opposite trend occurred for CAT activity. MDA content increased after 14-day exposure, and DNA damage was enhanced with an increase in the concentration of azoxystrobin. In conclusion, azoxystrobin caused oxidative stress leading to lipid peroxidation and DNA damage in earthworms. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/25011117/Integrated_assessment_of_oxidative_stress_and_DNA_damage_in_earthworms__Eisenia_fetida__exposed_to_azoxystrobin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(14)00262-0 DB - PRIME DP - Unbound Medicine ER -