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Virus infection of Haptolina ericina and Phaeocystis pouchetii implicates evolutionary conservation of programmed cell death induction in marine haptophyte-virus interactions.
J Plankton Res. 2014 Jul; 36(4):943-955.JP

Abstract

The mechanisms by which phytoplankton cope with stressors in the marine environment are neither fully characterized nor understood. As viruses are the most abundant entities in the global ocean and represent a strong top-down regulator of phytoplankton abundance and diversity, we sought to characterize the cellular response of two marine haptophytes to virus infection in order to gain more knowledge about the nature and diversity of microalgal responses to this chronic biotic stressor. We infected laboratory cultures of the haptophytes Haptolina ericina and Phaeocystis pouchetii with CeV-01B or PpV-01B dsDNA viruses, respectively, and assessed the extent to which host cellular responses resemble programmed cell death (PCD) through the activation of diagnostic molecular and biochemical markers. Pronounced DNA fragmentation and activation of cysteine aspartate-specific proteases (caspases) were only detected in virus-infected cultures of these phytoplankton. Inhibition of host caspase activity by addition of the pan-caspase inhibitor z-VAD-fmk did not impair virus production in either host-virus system, differentiating it from the Emiliania huxleyi-Coccolithovirus model of haptophyte-virus interactions. Nonetheless, our findings point to a general conservation of PCD-like activation during virus infection in ecologically diverse haptophytes, with the subtle heterogeneity of cell death biochemical responses possibly exerting differential regulation on phytoplankton abundance and diversity.

Authors+Show Affiliations

Uni Research As , Thormøhlensgt 49B, N-5006 Bergen , Norway ; Department of Biology , University of Bergen , Thormøhlensgt 53A, N-5006 Bergen , Norway.Institute of Marine and Coastal Sciences, Rutgers University , 71 Dudley Road, New Brunswick, NJ 08901 , USA.Department of Biology , University of Bergen , Thormøhlensgt 53A, N-5006 Bergen , Norway.Marine Chemistry and Geochemistry , Woods Hole Oceanographic Institution , Woods Hole, MA 02543 , USA.Marine Chemistry and Geochemistry , Woods Hole Oceanographic Institution , Woods Hole, MA 02543 , USA.Uni Research As , Thormøhlensgt 49B, N-5006 Bergen , Norway ; Department of Biology , University of Bergen , Thormøhlensgt 53A, N-5006 Bergen , Norway.Institute of Marine and Coastal Sciences, Rutgers University , 71 Dudley Road, New Brunswick, NJ 08901 , USA.Department of Biology , University of Bergen , Thormøhlensgt 53A, N-5006 Bergen , Norway.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25013242

Citation

Ray, Jessica L., et al. "Virus Infection of Haptolina Ericina and Phaeocystis Pouchetii Implicates Evolutionary Conservation of Programmed Cell Death Induction in Marine Haptophyte-virus Interactions." Journal of Plankton Research, vol. 36, no. 4, 2014, pp. 943-955.
Ray JL, Haramaty L, Thyrhaug R, et al. Virus infection of Haptolina ericina and Phaeocystis pouchetii implicates evolutionary conservation of programmed cell death induction in marine haptophyte-virus interactions. J Plankton Res. 2014;36(4):943-955.
Ray, J. L., Haramaty, L., Thyrhaug, R., Fredricks, H. F., Van Mooy, B. A., Larsen, A., Bidle, K. D., & Sandaa, R. A. (2014). Virus infection of Haptolina ericina and Phaeocystis pouchetii implicates evolutionary conservation of programmed cell death induction in marine haptophyte-virus interactions. Journal of Plankton Research, 36(4), 943-955.
Ray JL, et al. Virus Infection of Haptolina Ericina and Phaeocystis Pouchetii Implicates Evolutionary Conservation of Programmed Cell Death Induction in Marine Haptophyte-virus Interactions. J Plankton Res. 2014;36(4):943-955. PubMed PMID: 25013242.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Virus infection of Haptolina ericina and Phaeocystis pouchetii implicates evolutionary conservation of programmed cell death induction in marine haptophyte-virus interactions. AU - Ray,Jessica L, AU - Haramaty,Liti, AU - Thyrhaug,Runar, AU - Fredricks,Helen F, AU - Van Mooy,Benjamin A S, AU - Larsen,Aud, AU - Bidle,Kay D, AU - Sandaa,Ruth-Anne, Y1 - 2014/05/05/ PY - 2013/12/10/received PY - 2014/03/24/accepted PY - 2014/7/12/entrez PY - 2014/7/12/pubmed PY - 2014/7/12/medline KW - DNA fragmentation KW - IETD KW - Phycodnaviridae KW - caspase KW - haptophyte KW - z-VAD-fmk SP - 943 EP - 955 JF - Journal of plankton research JO - J Plankton Res VL - 36 IS - 4 N2 - The mechanisms by which phytoplankton cope with stressors in the marine environment are neither fully characterized nor understood. As viruses are the most abundant entities in the global ocean and represent a strong top-down regulator of phytoplankton abundance and diversity, we sought to characterize the cellular response of two marine haptophytes to virus infection in order to gain more knowledge about the nature and diversity of microalgal responses to this chronic biotic stressor. We infected laboratory cultures of the haptophytes Haptolina ericina and Phaeocystis pouchetii with CeV-01B or PpV-01B dsDNA viruses, respectively, and assessed the extent to which host cellular responses resemble programmed cell death (PCD) through the activation of diagnostic molecular and biochemical markers. Pronounced DNA fragmentation and activation of cysteine aspartate-specific proteases (caspases) were only detected in virus-infected cultures of these phytoplankton. Inhibition of host caspase activity by addition of the pan-caspase inhibitor z-VAD-fmk did not impair virus production in either host-virus system, differentiating it from the Emiliania huxleyi-Coccolithovirus model of haptophyte-virus interactions. Nonetheless, our findings point to a general conservation of PCD-like activation during virus infection in ecologically diverse haptophytes, with the subtle heterogeneity of cell death biochemical responses possibly exerting differential regulation on phytoplankton abundance and diversity. SN - 0142-7873 UR - https://www.unboundmedicine.com/medline/citation/25013242/Virus_infection_of_Haptolina_ericina_and_Phaeocystis_pouchetii_implicates_evolutionary_conservation_of_programmed_cell_death_induction_in_marine_haptophyte_virus_interactions_ L2 - https://academic.oup.com/plankt/article-lookup/doi/10.1093/plankt/fbu029 DB - PRIME DP - Unbound Medicine ER -