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Simultaneous determination and pharmacokinetics of five rhubarb anthraquinones in dog plasma by HPLC after orally administration the rhubarb extract.
Pak J Pharm Sci. 2014 Jul; 27(4):847-54.PJ

Abstract

Rhubarb is widely used in the treatment of obstipation, gastrointestinal indigestion and other diseases in China and other Asian countries for thousands of years. Anthraquinones are the major group of polyphenol constituents including aloe-emodin, rhein, emodin, chrysophanol and physcion. In order to evaluate the pharmacokinetics of five rhubarb anthraquinones, a high-performance liquid chromatography with fluorescence detection (HPLC-FLD) method for simultaneous determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in dog plasma was established. Solid phase extraction (SPE) was applied to the extraction and purification of samples. The calibration curves of five anthraquinones showed good linearity with r greater than 0.9925. The average extraction recoveries, examined at three concentration levels, carried from 92.1% to 102.3%, and the accuracies ranged from 87.7% to 102.5% with precision (RSD) <10%. The pharmacokinetic paremeters of five anthraquinones were investigated systematically after orally administration the rhubarb extract. Five anthraquinones were rapidly absorbed and Tmax for aloe-emodin, rhein, emodin, chrysophanol and physcion was at 0.75, 1.50, 0.75, 1.0 and 2.0 h respectively. The Cmax of five anthraquinones was 0.031, 3.39, 0.27, 0.036 and 0.032 μg/mL while the AUC of five anthraquinones was 0.35 ± 0.058, 32.22 ± 8.29, 2.97 ± 0.66, 0.43 ± 0.10 and 0.41 ± 0.12 mg h/L, respectively.

Authors+Show Affiliations

Feng SX
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China / College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, China.
Li JS
Institute of Geriatrics, Henan University of Traditional Chinese Medicine, Zhengzhou, China.
Wang SM
School of Traditional Chinese Materia Medica, Guangdong Pharmaceutical University, China.
Yang R
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China.
Zhou TQ
Institute of Geriatrics, Henan University of Traditional Chinese Medicine, Zhengzhou, China.
Li XY
College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, China.
Qu LB
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China.

MeSH

Administration, OralAnimalsAnthraquinonesChromatography, High Pressure LiquidDogsMalePlant ExtractsRheumSolid Phase Extraction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25015450

Citation

Feng, Su-xiang, et al. "Simultaneous Determination and Pharmacokinetics of Five Rhubarb Anthraquinones in Dog Plasma By HPLC After Orally Administration the Rhubarb Extract." Pakistan Journal of Pharmaceutical Sciences, vol. 27, no. 4, 2014, pp. 847-54.
Feng SX, Li JS, Wang SM, et al. Simultaneous determination and pharmacokinetics of five rhubarb anthraquinones in dog plasma by HPLC after orally administration the rhubarb extract. Pak J Pharm Sci. 2014;27(4):847-54.
Feng, S. X., Li, J. S., Wang, S. M., Yang, R., Zhou, T. Q., Li, X. Y., & Qu, L. B. (2014). Simultaneous determination and pharmacokinetics of five rhubarb anthraquinones in dog plasma by HPLC after orally administration the rhubarb extract. Pakistan Journal of Pharmaceutical Sciences, 27(4), 847-54.
Feng SX, et al. Simultaneous Determination and Pharmacokinetics of Five Rhubarb Anthraquinones in Dog Plasma By HPLC After Orally Administration the Rhubarb Extract. Pak J Pharm Sci. 2014;27(4):847-54. PubMed PMID: 25015450.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination and pharmacokinetics of five rhubarb anthraquinones in dog plasma by HPLC after orally administration the rhubarb extract. AU - Feng,Su-xiang, AU - Li,Jian-sheng, AU - Wang,Shu-mei, AU - Yang,Ran, AU - Zhou,Ti-qiang, AU - Li,Xiao-yu, AU - Qu,Ling-bo, PY - 2014/7/13/entrez PY - 2014/7/13/pubmed PY - 2014/9/19/medline SP - 847 EP - 54 JF - Pakistan journal of pharmaceutical sciences JO - Pak J Pharm Sci VL - 27 IS - 4 N2 - Rhubarb is widely used in the treatment of obstipation, gastrointestinal indigestion and other diseases in China and other Asian countries for thousands of years. Anthraquinones are the major group of polyphenol constituents including aloe-emodin, rhein, emodin, chrysophanol and physcion. In order to evaluate the pharmacokinetics of five rhubarb anthraquinones, a high-performance liquid chromatography with fluorescence detection (HPLC-FLD) method for simultaneous determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in dog plasma was established. Solid phase extraction (SPE) was applied to the extraction and purification of samples. The calibration curves of five anthraquinones showed good linearity with r greater than 0.9925. The average extraction recoveries, examined at three concentration levels, carried from 92.1% to 102.3%, and the accuracies ranged from 87.7% to 102.5% with precision (RSD) <10%. The pharmacokinetic paremeters of five anthraquinones were investigated systematically after orally administration the rhubarb extract. Five anthraquinones were rapidly absorbed and Tmax for aloe-emodin, rhein, emodin, chrysophanol and physcion was at 0.75, 1.50, 0.75, 1.0 and 2.0 h respectively. The Cmax of five anthraquinones was 0.031, 3.39, 0.27, 0.036 and 0.032 μg/mL while the AUC of five anthraquinones was 0.35 ± 0.058, 32.22 ± 8.29, 2.97 ± 0.66, 0.43 ± 0.10 and 0.41 ± 0.12 mg h/L, respectively. SN - 1011-601X UR - https://www.unboundmedicine.com/medline/citation/25015450/Simultaneous_determination_and_pharmacokinetics_of_five_rhubarb_anthraquinones_in_dog_plasma_by_HPLC_after_orally_administration_the_rhubarb_extract_ DB - PRIME DP - Unbound Medicine ER -