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In vivo interactions between cobalt or ferric compounds and the pools of sulphide in the blood during and after H2S poisoning.
Toxicol Sci 2014; 141(2):493-504TS

Abstract

Hydrogen sulphide (H2S), a chemical hazard in oil and gas production, has recently become a dreadful method of suicide, posing specific risks and challenges for the first responders. Currently, there is no proven effective treatment against H2S poisoning and its severe neurological, respiratory or cardiac after-effects. We have recently described that H2S is present in various compartments, or pools, in the body during sulphide exposure, which have different levels of toxicity. The general goals of our study were to (1) determine the concentrations and kinetics of the various pools of hydrogen sulphide in the blood, i.e., gaseous (CgH2S) versus total sulphide, i.e., reacting with monobromobimane (CMBBH2S), during and following H2S exposure in a small and large mammal and (2) establish the interaction between the pools of H2S and a methemoglobin (MetHb) solution or a high dose of hydroxocobalamin (HyCo). We found that CgH2S during and following H2S infusion was similar in sedated sheep and rats at any given rate of infusion/kg and provoked symptoms, i.e., hyperpnea and apnea, at the same CgH2S. After H2S administration was stopped, CgH2S disappeared within 1 min. CMBBH2S also dropped to 2-3μM, but remained above baseline levels for at least 30 min. Infusion of a MetHb solution during H2S infusion produced an immediate reduction in the free/soluble pool of H2S only, whereas CMBBH2S increased by severalfold. HyCo (70 mg/kg) also decreased the concentrations of free/soluble H2S to almost zero; CgH2S returned to pre-HyCo levels within a maximum of 20 min, if H2S infusion is maintained. These results are discussed in the context of a relevant scenario, wherein antidotes can only be administered after H2S exposure.

Authors+Show Affiliations

Department of Medicine, Division of Pulmonary and Critical Care Medicine, Penn State University College of Medicine, Hershey, PA 17033 phaouzi@hmc.psu.edu.Department of Medicine, Division of Pulmonary and Critical Care Medicine, Penn State University College of Medicine, Hershey, PA 17033.Department of Medicine, Division of Pulmonary and Critical Care Medicine, Penn State University College of Medicine, Hershey, PA 17033.Department of Pharmacology, Penn State University College of Medicine, Hershey, PA 17033.Department of Medicine, Division of Pulmonary and Critical Care Medicine, Penn State University College of Medicine, Hershey, PA 17033.Department of Medicine, Division of Pulmonary and Critical Care Medicine, Penn State University College of Medicine, Hershey, PA 17033 Department of Biological and Allied Health Sciences, Bloomsburg University, Bloomsburg, PA 17815.

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25015662

Citation

Haouzi, Philippe, et al. "In Vivo Interactions Between Cobalt or Ferric Compounds and the Pools of Sulphide in the Blood During and After H2S Poisoning." Toxicological Sciences : an Official Journal of the Society of Toxicology, vol. 141, no. 2, 2014, pp. 493-504.
Haouzi P, Sonobe T, Torsell-Tubbs N, et al. In vivo interactions between cobalt or ferric compounds and the pools of sulphide in the blood during and after H2S poisoning. Toxicol Sci. 2014;141(2):493-504.
Haouzi, P., Sonobe, T., Torsell-Tubbs, N., Prokopczyk, B., Chenuel, B., & Klingerman, C. M. (2014). In vivo interactions between cobalt or ferric compounds and the pools of sulphide in the blood during and after H2S poisoning. Toxicological Sciences : an Official Journal of the Society of Toxicology, 141(2), pp. 493-504. doi:10.1093/toxsci/kfu140.
Haouzi P, et al. In Vivo Interactions Between Cobalt or Ferric Compounds and the Pools of Sulphide in the Blood During and After H2S Poisoning. Toxicol Sci. 2014;141(2):493-504. PubMed PMID: 25015662.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo interactions between cobalt or ferric compounds and the pools of sulphide in the blood during and after H2S poisoning. AU - Haouzi,Philippe, AU - Sonobe,Takashi, AU - Torsell-Tubbs,Nicole, AU - Prokopczyk,Bogdan, AU - Chenuel,Bruno, AU - Klingerman,Candice M, Y1 - 2014/07/11/ PY - 2014/7/13/entrez PY - 2014/7/13/pubmed PY - 2015/11/3/medline SP - 493 EP - 504 JF - Toxicological sciences : an official journal of the Society of Toxicology JO - Toxicol. Sci. VL - 141 IS - 2 N2 - Hydrogen sulphide (H2S), a chemical hazard in oil and gas production, has recently become a dreadful method of suicide, posing specific risks and challenges for the first responders. Currently, there is no proven effective treatment against H2S poisoning and its severe neurological, respiratory or cardiac after-effects. We have recently described that H2S is present in various compartments, or pools, in the body during sulphide exposure, which have different levels of toxicity. The general goals of our study were to (1) determine the concentrations and kinetics of the various pools of hydrogen sulphide in the blood, i.e., gaseous (CgH2S) versus total sulphide, i.e., reacting with monobromobimane (CMBBH2S), during and following H2S exposure in a small and large mammal and (2) establish the interaction between the pools of H2S and a methemoglobin (MetHb) solution or a high dose of hydroxocobalamin (HyCo). We found that CgH2S during and following H2S infusion was similar in sedated sheep and rats at any given rate of infusion/kg and provoked symptoms, i.e., hyperpnea and apnea, at the same CgH2S. After H2S administration was stopped, CgH2S disappeared within 1 min. CMBBH2S also dropped to 2-3μM, but remained above baseline levels for at least 30 min. Infusion of a MetHb solution during H2S infusion produced an immediate reduction in the free/soluble pool of H2S only, whereas CMBBH2S increased by severalfold. HyCo (70 mg/kg) also decreased the concentrations of free/soluble H2S to almost zero; CgH2S returned to pre-HyCo levels within a maximum of 20 min, if H2S infusion is maintained. These results are discussed in the context of a relevant scenario, wherein antidotes can only be administered after H2S exposure. SN - 1096-0929 UR - https://www.unboundmedicine.com/medline/citation/25015662/In_vivo_interactions_between_cobalt_or_ferric_compounds_and_the_pools_of_sulphide_in_the_blood_during_and_after_H2S_poisoning_ L2 - https://academic.oup.com/toxsci/article-lookup/doi/10.1093/toxsci/kfu140 DB - PRIME DP - Unbound Medicine ER -