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In vitro activity of MCB3681 against Clostridium difficile strains.
Anaerobe. 2014 Aug; 28:216-9.A

Abstract

One hundred fourteen Clostridium difficile strains were collected from 67 patients and analyzed for the presence of C. difficile toxin B by the cell cytotoxoicity neutralization assay, genes for toxin A, toxin B, binary toxin and TcdC deletion by PCR. All strains were also PCR-ribotyped. The MICs of the isolates were determined against MCB3681 and nine other antimicrobial agents by the agar dilution method. All isolates were positive for toxin B as well as for toxin A and B genes. In addition, 13 isolates were positive for the binary toxin genes. Thirty-two different ribotypes were identified. No strain of ribotype 027 was found. All 114 isolates were sensitive to MCB3681 (0.008-0.5 mg/l), cadazolid (0.064-0.5 mg/l), fidaxomicin (0.008-0.125 mg/l), metronidazole (0.125-2 mg/l), vancomycin (0.125-1 mg/l) and tigecycline (0.032-0.25 mg/l). Three isolates were resistant to linezolid (8 mg/l), 12 isolates were resistant to moxifloxacin (8-32 mg/l), 87 isolates were resistant to clindamycin (8-256 mg/l) and 107 isolates were resistant to ciprofloxacin (8-256 mg/l). No association between toxins A, B and binary toxin, ribotypes and the sensitivity to MCB3681 could be found. MCB3681 has a potent in vitro activity against C. difficile.

Authors+Show Affiliations

Department of Laboratory Medicine, Karolinska University Hospital, Karolinska Institutet, SE-141 86 Stockholm, Sweden.University Medical Center Schleswig-Holstein, Institute for Infection Medicine, Brunswiker Str. 4, D-24105 Kiel, Germany.Department of Laboratory Medicine, Karolinska University Hospital, Karolinska Institutet, SE-141 86 Stockholm, Sweden.Department of Laboratory Medicine, Karolinska University Hospital, Karolinska Institutet, SE-141 86 Stockholm, Sweden. Electronic address: carl.erik.nord@ki.se.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25016084

Citation

Rashid, Mamun-Ur, et al. "In Vitro Activity of MCB3681 Against Clostridium Difficile Strains." Anaerobe, vol. 28, 2014, pp. 216-9.
Rashid MU, Dalhoff A, Weintraub A, et al. In vitro activity of MCB3681 against Clostridium difficile strains. Anaerobe. 2014;28:216-9.
Rashid, M. U., Dalhoff, A., Weintraub, A., & Nord, C. E. (2014). In vitro activity of MCB3681 against Clostridium difficile strains. Anaerobe, 28, 216-9. https://doi.org/10.1016/j.anaerobe.2014.07.001
Rashid MU, et al. In Vitro Activity of MCB3681 Against Clostridium Difficile Strains. Anaerobe. 2014;28:216-9. PubMed PMID: 25016084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro activity of MCB3681 against Clostridium difficile strains. AU - Rashid,Mamun-Ur, AU - Dalhoff,Axel, AU - Weintraub,Andrej, AU - Nord,Carl Erik, Y1 - 2014/07/10/ PY - 2014/06/09/received PY - 2014/06/30/revised PY - 2014/07/02/accepted PY - 2014/7/13/entrez PY - 2014/7/13/pubmed PY - 2015/4/2/medline KW - Clostridium difficile infections KW - In vitro activity KW - MCB3681, MCB3837 SP - 216 EP - 9 JF - Anaerobe JO - Anaerobe VL - 28 N2 - One hundred fourteen Clostridium difficile strains were collected from 67 patients and analyzed for the presence of C. difficile toxin B by the cell cytotoxoicity neutralization assay, genes for toxin A, toxin B, binary toxin and TcdC deletion by PCR. All strains were also PCR-ribotyped. The MICs of the isolates were determined against MCB3681 and nine other antimicrobial agents by the agar dilution method. All isolates were positive for toxin B as well as for toxin A and B genes. In addition, 13 isolates were positive for the binary toxin genes. Thirty-two different ribotypes were identified. No strain of ribotype 027 was found. All 114 isolates were sensitive to MCB3681 (0.008-0.5 mg/l), cadazolid (0.064-0.5 mg/l), fidaxomicin (0.008-0.125 mg/l), metronidazole (0.125-2 mg/l), vancomycin (0.125-1 mg/l) and tigecycline (0.032-0.25 mg/l). Three isolates were resistant to linezolid (8 mg/l), 12 isolates were resistant to moxifloxacin (8-32 mg/l), 87 isolates were resistant to clindamycin (8-256 mg/l) and 107 isolates were resistant to ciprofloxacin (8-256 mg/l). No association between toxins A, B and binary toxin, ribotypes and the sensitivity to MCB3681 could be found. MCB3681 has a potent in vitro activity against C. difficile. SN - 1095-8274 UR - https://www.unboundmedicine.com/medline/citation/25016084/In_vitro_activity_of_MCB3681_against_Clostridium_difficile_strains_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1075-9964(14)00087-0 DB - PRIME DP - Unbound Medicine ER -