Tags

Type your tag names separated by a space and hit enter

Bioassay-guided isolation and identification of anti-platelet-active compounds from the root of Ashitaba (Angelica keiskei Koidz.).
Nat Prod Res 2014; 28(24):2312-6NP

Abstract

Platelet aggregation is fundamental to a wide range of physiological and pathological processes, including the induction of thrombosis and arteriosclerosis. Anti-platelet activity of a crude methanol extract and solvent fractions of Ashitaba roots (Angelica keiskei Koidz.) was evaluated using a turbidimetric method using washed rabbit platelets. We identified the anti-platelet activities of two chalcones, 4-hydroxyderricin and xanthoangelol, isolated from the ethyl acetate-soluble fraction of Ashitaba roots by using a bioassay-guided isolation method. 4-Hydroxyderricin and xanthoangelol effectively inhibited platelet aggregation induced by collagen (IC50 of 41.9 and 35.9 μM, respectively), platelet-activating factor (IC50 of 46.1 and 42.3 μM, respectively) and phorbol 12-myristate 13-acetate (IC50 of 16.5 and 45.9 μM, respectively). These compounds did not inhibit thrombin-induced platelet aggregation (IC50 of>80 μM). The results suggest that the chalcones 4-hydroxyderricin and xanthoangelol may be potent anti-thrombotic components of A. keiskei Koidz.

Authors+Show Affiliations

a School of Applied Bioscience, Kyungpook National University , Daegu 702-701 , South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25019280

Citation

Son, Dong Ju, et al. "Bioassay-guided Isolation and Identification of Anti-platelet-active Compounds From the Root of Ashitaba (Angelica Keiskei Koidz.)." Natural Product Research, vol. 28, no. 24, 2014, pp. 2312-6.
Son DJ, Park YO, Yu C, et al. Bioassay-guided isolation and identification of anti-platelet-active compounds from the root of Ashitaba (Angelica keiskei Koidz.). Nat Prod Res. 2014;28(24):2312-6.
Son, D. J., Park, Y. O., Yu, C., Lee, S. E., & Park, Y. H. (2014). Bioassay-guided isolation and identification of anti-platelet-active compounds from the root of Ashitaba (Angelica keiskei Koidz.). Natural Product Research, 28(24), pp. 2312-6. doi:10.1080/14786419.2014.931389.
Son DJ, et al. Bioassay-guided Isolation and Identification of Anti-platelet-active Compounds From the Root of Ashitaba (Angelica Keiskei Koidz.). Nat Prod Res. 2014;28(24):2312-6. PubMed PMID: 25019280.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioassay-guided isolation and identification of anti-platelet-active compounds from the root of Ashitaba (Angelica keiskei Koidz.). AU - Son,Dong Ju, AU - Park,Ye Oak, AU - Yu,Chengguang, AU - Lee,Sung Eun, AU - Park,Young Hyun, Y1 - 2014/07/14/ PY - 2014/7/15/entrez PY - 2014/7/16/pubmed PY - 2015/2/24/medline KW - 4-hydroxyderricin KW - Angelica keiskei Koidaz KW - Ashitaba KW - anti-platelet KW - xanthoangelol SP - 2312 EP - 6 JF - Natural product research JO - Nat. Prod. Res. VL - 28 IS - 24 N2 - Platelet aggregation is fundamental to a wide range of physiological and pathological processes, including the induction of thrombosis and arteriosclerosis. Anti-platelet activity of a crude methanol extract and solvent fractions of Ashitaba roots (Angelica keiskei Koidz.) was evaluated using a turbidimetric method using washed rabbit platelets. We identified the anti-platelet activities of two chalcones, 4-hydroxyderricin and xanthoangelol, isolated from the ethyl acetate-soluble fraction of Ashitaba roots by using a bioassay-guided isolation method. 4-Hydroxyderricin and xanthoangelol effectively inhibited platelet aggregation induced by collagen (IC50 of 41.9 and 35.9 μM, respectively), platelet-activating factor (IC50 of 46.1 and 42.3 μM, respectively) and phorbol 12-myristate 13-acetate (IC50 of 16.5 and 45.9 μM, respectively). These compounds did not inhibit thrombin-induced platelet aggregation (IC50 of>80 μM). The results suggest that the chalcones 4-hydroxyderricin and xanthoangelol may be potent anti-thrombotic components of A. keiskei Koidz. SN - 1478-6427 UR - https://www.unboundmedicine.com/medline/citation/25019280/Bioassay_guided_isolation_and_identification_of_anti_platelet_active_compounds_from_the_root_of_Ashitaba__Angelica_keiskei_Koidz___ L2 - http://www.tandfonline.com/doi/full/10.1080/14786419.2014.931389 DB - PRIME DP - Unbound Medicine ER -