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Supercritical fluid extraction (SFE) of cranberries does not extract oligomeric proanthocyanidins (PAC) but does alter the chromatography and bioactivity of PAC fractions extracted from SFE residues.
J Agric Food Chem 2014; 62(31):7730-7JA

Abstract

Supercritical fluid extraction (SFE) removed lipophilic compounds and low molecular weight flavonoids from cranberries. However, SFE did not extract proanthocyanidins (PAC). The SFE PAC-enriched residue was submitted to fractionation on Sephadex LH-20 using ethanol, ethanol/methanol, and 80% acetone. PAC degree of polymerization (DP) and ratios of "A-type" to "B-type" interflavan bonds were compared with those of PAC fractions without SFE. Mass spectrometry showed that when SFE was used, PAC distribution was shifted toward higher DP and contained higher amounts of two and three "A-type" bonds compared to PAC fractions without SFE. The 80% acetone fraction with SFE had significantly greater extraintestinal pathogenic Escherichia coli (ExPEC) agglutination and significantly lower ExPEC invasion of enterocytes than the fraction without SFE. Cranberry PAC with higher numbers of "A-type" interflavan bonds are more bioactive in agglutinating ExPEC and inhibiting ExPEC enterocyte invasion.

Authors+Show Affiliations

Department of Food Science, University of Wisconsin-Madison , 1605 Linden Drive, Madison, Wisconsin 53706, United States.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

25019644

Citation

Feliciano, Rodrigo P., et al. "Supercritical Fluid Extraction (SFE) of Cranberries Does Not Extract Oligomeric Proanthocyanidins (PAC) but Does Alter the Chromatography and Bioactivity of PAC Fractions Extracted From SFE Residues." Journal of Agricultural and Food Chemistry, vol. 62, no. 31, 2014, pp. 7730-7.
Feliciano RP, Meudt JJ, Shanmuganayagam D, et al. Supercritical fluid extraction (SFE) of cranberries does not extract oligomeric proanthocyanidins (PAC) but does alter the chromatography and bioactivity of PAC fractions extracted from SFE residues. J Agric Food Chem. 2014;62(31):7730-7.
Feliciano, R. P., Meudt, J. J., Shanmuganayagam, D., Metzger, B. T., Krueger, C. G., & Reed, J. D. (2014). Supercritical fluid extraction (SFE) of cranberries does not extract oligomeric proanthocyanidins (PAC) but does alter the chromatography and bioactivity of PAC fractions extracted from SFE residues. Journal of Agricultural and Food Chemistry, 62(31), pp. 7730-7. doi:10.1021/jf502296b.
Feliciano RP, et al. Supercritical Fluid Extraction (SFE) of Cranberries Does Not Extract Oligomeric Proanthocyanidins (PAC) but Does Alter the Chromatography and Bioactivity of PAC Fractions Extracted From SFE Residues. J Agric Food Chem. 2014 Aug 6;62(31):7730-7. PubMed PMID: 25019644.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Supercritical fluid extraction (SFE) of cranberries does not extract oligomeric proanthocyanidins (PAC) but does alter the chromatography and bioactivity of PAC fractions extracted from SFE residues. AU - Feliciano,Rodrigo P, AU - Meudt,Jennifer J, AU - Shanmuganayagam,Dhanansayan, AU - Metzger,Brandon T, AU - Krueger,Christian G, AU - Reed,Jess D, Y1 - 2014/07/24/ PY - 2014/7/15/entrez PY - 2014/7/16/pubmed PY - 2015/4/14/medline SP - 7730 EP - 7 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 62 IS - 31 N2 - Supercritical fluid extraction (SFE) removed lipophilic compounds and low molecular weight flavonoids from cranberries. However, SFE did not extract proanthocyanidins (PAC). The SFE PAC-enriched residue was submitted to fractionation on Sephadex LH-20 using ethanol, ethanol/methanol, and 80% acetone. PAC degree of polymerization (DP) and ratios of "A-type" to "B-type" interflavan bonds were compared with those of PAC fractions without SFE. Mass spectrometry showed that when SFE was used, PAC distribution was shifted toward higher DP and contained higher amounts of two and three "A-type" bonds compared to PAC fractions without SFE. The 80% acetone fraction with SFE had significantly greater extraintestinal pathogenic Escherichia coli (ExPEC) agglutination and significantly lower ExPEC invasion of enterocytes than the fraction without SFE. Cranberry PAC with higher numbers of "A-type" interflavan bonds are more bioactive in agglutinating ExPEC and inhibiting ExPEC enterocyte invasion. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/25019644/Supercritical_fluid_extraction__SFE__of_cranberries_does_not_extract_oligomeric_proanthocyanidins__PAC__but_does_alter_the_chromatography_and_bioactivity_of_PAC_fractions_extracted_from_SFE_residues_ L2 - https://dx.doi.org/10.1021/jf502296b DB - PRIME DP - Unbound Medicine ER -