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Overoxidized polypyrrole/graphene nanocomposite with good electrochemical performance as novel electrode material for the detection of adenine and guanine.
Biosens Bioelectron. 2014 Dec 15; 62:261-7.BB

Abstract

Most conducting polymer/graphene composites have excellent electrical conductivity. However, the background currents of these composites modified electrodes are much larger. In order to improve the sensitivities of these methods, it is necessary to decrease the background signal. In this paper, porous structure films of overoxidized polypyrrole/graphene (PPyox/GR) have been electrochemically coated onto glassy carbon electrode (GCE) and successfully utilized as an efficient electrode material for the quantitive detection of adenine and guanine, two of the most important components of DNA and RNA. The permselective polymer coatings with low background current could improve the selectivity and sensitivity of microelectrodes for the electropositive purine bases. The GRs into these polymers would further improve sensitivity by increasing the electroactive surface area. The electrochemical sensor can be applied to the quantification of adenine and guanine with a linear range covering 0.06-100 µM and 0.04-100 µM, and a low detection limit of 0.02 μM and 0.01 μM, respectively. More importantly, the proposed method was applied to quantify adenine and guanine in calf thymus DNA with satisfactory results.

Authors+Show Affiliations

Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, PR China.Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, PR China. Electronic address: xujingkun@tsinghua.org.cn.College of Science, Jiangxi Agricultural University, Nanchang 330045, PR China. Electronic address: lulimin816@hotmail.com.Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, PR China.Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, PR China.Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, PR China; College of Science, Jiangxi Agricultural University, Nanchang 330045, PR China.Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, PR China.Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, PR China.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25022509

Citation

Gao, Yan-Sha, et al. "Overoxidized Polypyrrole/graphene Nanocomposite With Good Electrochemical Performance as Novel Electrode Material for the Detection of Adenine and Guanine." Biosensors & Bioelectronics, vol. 62, 2014, pp. 261-7.
Gao YS, Xu JK, Lu LM, et al. Overoxidized polypyrrole/graphene nanocomposite with good electrochemical performance as novel electrode material for the detection of adenine and guanine. Biosens Bioelectron. 2014;62:261-7.
Gao, Y. S., Xu, J. K., Lu, L. M., Wu, L. P., Zhang, K. X., Nie, T., Zhu, X. F., & Wu, Y. (2014). Overoxidized polypyrrole/graphene nanocomposite with good electrochemical performance as novel electrode material for the detection of adenine and guanine. Biosensors & Bioelectronics, 62, 261-7. https://doi.org/10.1016/j.bios.2014.06.044
Gao YS, et al. Overoxidized Polypyrrole/graphene Nanocomposite With Good Electrochemical Performance as Novel Electrode Material for the Detection of Adenine and Guanine. Biosens Bioelectron. 2014 Dec 15;62:261-7. PubMed PMID: 25022509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overoxidized polypyrrole/graphene nanocomposite with good electrochemical performance as novel electrode material for the detection of adenine and guanine. AU - Gao,Yan-Sha, AU - Xu,Jing-Kun, AU - Lu,Li-Min, AU - Wu,Li-Ping, AU - Zhang,Kai-Xin, AU - Nie,Tao, AU - Zhu,Xiao-Fei, AU - Wu,Yao, Y1 - 2014/06/26/ PY - 2014/04/10/received PY - 2014/06/12/revised PY - 2014/06/19/accepted PY - 2014/7/16/entrez PY - 2014/7/16/pubmed PY - 2015/9/17/medline KW - Adenine KW - Electrochemical detection KW - Graphene KW - Guanine KW - Overoxidized polypyrrole SP - 261 EP - 7 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 62 N2 - Most conducting polymer/graphene composites have excellent electrical conductivity. However, the background currents of these composites modified electrodes are much larger. In order to improve the sensitivities of these methods, it is necessary to decrease the background signal. In this paper, porous structure films of overoxidized polypyrrole/graphene (PPyox/GR) have been electrochemically coated onto glassy carbon electrode (GCE) and successfully utilized as an efficient electrode material for the quantitive detection of adenine and guanine, two of the most important components of DNA and RNA. The permselective polymer coatings with low background current could improve the selectivity and sensitivity of microelectrodes for the electropositive purine bases. The GRs into these polymers would further improve sensitivity by increasing the electroactive surface area. The electrochemical sensor can be applied to the quantification of adenine and guanine with a linear range covering 0.06-100 µM and 0.04-100 µM, and a low detection limit of 0.02 μM and 0.01 μM, respectively. More importantly, the proposed method was applied to quantify adenine and guanine in calf thymus DNA with satisfactory results. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/25022509/Overoxidized_polypyrrole/graphene_nanocomposite_with_good_electrochemical_performance_as_novel_electrode_material_for_the_detection_of_adenine_and_guanine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(14)00457-6 DB - PRIME DP - Unbound Medicine ER -