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A fatty acid condensing enzyme from Physaria fendleri increases hydroxy fatty acid accumulation in transgenic oilseeds of Camelina sativa.
Planta. 2014 Sep; 240(3):599-610.P

Abstract

MAIN CONCLUSION

Co-expression of a lesquerella fatty acid elongase and the castor fatty acid hydroxylase in camelina results in higher hydroxy fatty acid containing seeds with normal oil content and viability. Producing hydroxy fatty acids (HFA) in oilseed crops has been a long-standing goal to replace castor oil as a renewable source for numerous industrial applications. A fatty acid hydroxylase, RcFAH, from Ricinus communis, was introduced into Camelina sativa, but yielded only 15 % of HFA in its seed oil, much lower than the 90 % found in castor bean. Furthermore, the transgenic seeds contained decreased oil content and the germination ability was severely affected. Interestingly, HFA accumulation was significantly increased in camelina seed when co-expressing RcFAH with a fatty acid condensing enzyme, LfKCS3, from Physaria fendleri, a native HFA accumulator relative to camelina. The oil content and seed germination of the transgenic seeds also appeared normal compared to non-transgenics. LfKCS3 has been previously characterized to specifically elongate the hydroxylated ricinoleic acid to lesquerolic acid, the 20-carbon HFA found in lesquerella oil. The elongation reaction may facilitate the HFA flux from phosphatidylcholine (PC), the site of HFA formation, into the acyl-CoA pool for more efficient utilization in triacylglycerol (TAG) biosynthesis. This was demonstrated by increased HFA accumulation in TAG concurrent with reduced HFA content in PC during camelina seed development, and increased C20-HFA in HFA-TAG molecules. These effects of LfKCS3 thus may effectively relieve the bottleneck for HFA utilization in TAG biosynthesis and the feedback inhibition to fatty acid synthesis, result in higher HFA accumulation and restore oil content and seed viability.

Authors+Show Affiliations

Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT, 59717-3150, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

25023632

Citation

Snapp, Anna R., et al. "A Fatty Acid Condensing Enzyme From Physaria Fendleri Increases Hydroxy Fatty Acid Accumulation in Transgenic Oilseeds of Camelina Sativa." Planta, vol. 240, no. 3, 2014, pp. 599-610.
Snapp AR, Kang J, Qi X, et al. A fatty acid condensing enzyme from Physaria fendleri increases hydroxy fatty acid accumulation in transgenic oilseeds of Camelina sativa. Planta. 2014;240(3):599-610.
Snapp, A. R., Kang, J., Qi, X., & Lu, C. (2014). A fatty acid condensing enzyme from Physaria fendleri increases hydroxy fatty acid accumulation in transgenic oilseeds of Camelina sativa. Planta, 240(3), 599-610. https://doi.org/10.1007/s00425-014-2122-2
Snapp AR, et al. A Fatty Acid Condensing Enzyme From Physaria Fendleri Increases Hydroxy Fatty Acid Accumulation in Transgenic Oilseeds of Camelina Sativa. Planta. 2014;240(3):599-610. PubMed PMID: 25023632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A fatty acid condensing enzyme from Physaria fendleri increases hydroxy fatty acid accumulation in transgenic oilseeds of Camelina sativa. AU - Snapp,Anna R, AU - Kang,Jinling, AU - Qi,Xiaoli, AU - Lu,Chaofu, Y1 - 2014/07/15/ PY - 2014/05/19/received PY - 2014/07/04/accepted PY - 2014/7/16/entrez PY - 2014/7/16/pubmed PY - 2015/11/10/medline SP - 599 EP - 610 JF - Planta JO - Planta VL - 240 IS - 3 N2 - MAIN CONCLUSION: Co-expression of a lesquerella fatty acid elongase and the castor fatty acid hydroxylase in camelina results in higher hydroxy fatty acid containing seeds with normal oil content and viability. Producing hydroxy fatty acids (HFA) in oilseed crops has been a long-standing goal to replace castor oil as a renewable source for numerous industrial applications. A fatty acid hydroxylase, RcFAH, from Ricinus communis, was introduced into Camelina sativa, but yielded only 15 % of HFA in its seed oil, much lower than the 90 % found in castor bean. Furthermore, the transgenic seeds contained decreased oil content and the germination ability was severely affected. Interestingly, HFA accumulation was significantly increased in camelina seed when co-expressing RcFAH with a fatty acid condensing enzyme, LfKCS3, from Physaria fendleri, a native HFA accumulator relative to camelina. The oil content and seed germination of the transgenic seeds also appeared normal compared to non-transgenics. LfKCS3 has been previously characterized to specifically elongate the hydroxylated ricinoleic acid to lesquerolic acid, the 20-carbon HFA found in lesquerella oil. The elongation reaction may facilitate the HFA flux from phosphatidylcholine (PC), the site of HFA formation, into the acyl-CoA pool for more efficient utilization in triacylglycerol (TAG) biosynthesis. This was demonstrated by increased HFA accumulation in TAG concurrent with reduced HFA content in PC during camelina seed development, and increased C20-HFA in HFA-TAG molecules. These effects of LfKCS3 thus may effectively relieve the bottleneck for HFA utilization in TAG biosynthesis and the feedback inhibition to fatty acid synthesis, result in higher HFA accumulation and restore oil content and seed viability. SN - 1432-2048 UR - https://www.unboundmedicine.com/medline/citation/25023632/A_fatty_acid_condensing_enzyme_from_Physaria_fendleri_increases_hydroxy_fatty_acid_accumulation_in_transgenic_oilseeds_of_Camelina_sativa_ L2 - https://dx.doi.org/10.1007/s00425-014-2122-2 DB - PRIME DP - Unbound Medicine ER -