Tags

Type your tag names separated by a space and hit enter

Gastroprotective activity of polyphenolic-rich extract of Potentilla mooniana.
Pharm Biol. 2014 Dec; 52(12):1532-42.PB

Abstract

CONTEXT

Potentilla mooniana Wight. (Rosaceae) is a plant found in the Himalayan region where the root is traditionally used to treat stomach problems including gastric-ulcer.

OBJECTIVE

To scientifically validate the gastro-protective effect and derive the possible mechanistic activity of the ethanol root extract from P. mooniana (EPM).

MATERIALS AND METHODS

The gastroprotective effect of EPM (100-400 mg/kg, p.o.) was evaluated on both the physical (Pyloric ligation, PL; Cold restrain stress, CRS) and chemical (absolute ethanol, EtOH; aspirin, ASP) ulcerogens induced ulceration in rats. The mechanistic activity of EPM was tested on various gastric-ulcer parameters, namely gastric pH, volume, acid-pepsin output, DNA content, histamine level, H(+)K(+)-ATPase activity, mucus content, microvascular permeability, antioxidant markers, and gastric-histopathological study.

RESULTS

EPM significantly reduces the ulcer score against all the four tested gastric-ulcer models. In the PL model, EPM showed significant reduction (p < 0.05) in acid-pepsin output and cell shedding; however, no significant effect was observed on gastric volume, cell proliferation, stomach glandular weight, and histamine levels. EPM (400 mg/kg, p.o.) when compared with ulcer control showed significant increase in gastric pH by 41.6% and decrease in H(+)K(+)-ATPase activity by 47.73%. In addition, EPM showed significant increase in mucus content by 58.60% and a decrease in the microvascular permeability of Evans Blue by 85.00%, justifying its protective effects. Furthermore, EPM also showed significant antioxidant activity and histopathologically possessed excellent cytoprotective effect.

CONCLUSION

The gastro-protective effect of EPM is attributed mainly to the defensive mechanism owing to the presence of a good quantity of polyphenolic components.

Authors+Show Affiliations

Department of Pharmaceutics, Indian Institute of Technology (Banaras Hindu University) , Varanasi, Uttar Pradesh , India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25026332

Citation

Laloo, Damiki, et al. "Gastroprotective Activity of Polyphenolic-rich Extract of Potentilla Mooniana." Pharmaceutical Biology, vol. 52, no. 12, 2014, pp. 1532-42.
Laloo D, Prasad SK, Sairam K, et al. Gastroprotective activity of polyphenolic-rich extract of Potentilla mooniana. Pharm Biol. 2014;52(12):1532-42.
Laloo, D., Prasad, S. K., Sairam, K., & Hemalatha, S. (2014). Gastroprotective activity of polyphenolic-rich extract of Potentilla mooniana. Pharmaceutical Biology, 52(12), 1532-42. https://doi.org/10.3109/13880209.2014.905794
Laloo D, et al. Gastroprotective Activity of Polyphenolic-rich Extract of Potentilla Mooniana. Pharm Biol. 2014;52(12):1532-42. PubMed PMID: 25026332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gastroprotective activity of polyphenolic-rich extract of Potentilla mooniana. AU - Laloo,Damiki, AU - Prasad,Satyendra K, AU - Sairam,Krishnamurthy, AU - Hemalatha,S, Y1 - 2014/07/15/ PY - 2014/7/16/entrez PY - 2014/7/16/pubmed PY - 2016/1/9/medline KW - Antioxidant KW - ethanol KW - gastric-ulcer KW - histopathology KW - mucus KW - polysaccharides KW - pyloric ligation SP - 1532 EP - 42 JF - Pharmaceutical biology JO - Pharm Biol VL - 52 IS - 12 N2 - CONTEXT: Potentilla mooniana Wight. (Rosaceae) is a plant found in the Himalayan region where the root is traditionally used to treat stomach problems including gastric-ulcer. OBJECTIVE: To scientifically validate the gastro-protective effect and derive the possible mechanistic activity of the ethanol root extract from P. mooniana (EPM). MATERIALS AND METHODS: The gastroprotective effect of EPM (100-400 mg/kg, p.o.) was evaluated on both the physical (Pyloric ligation, PL; Cold restrain stress, CRS) and chemical (absolute ethanol, EtOH; aspirin, ASP) ulcerogens induced ulceration in rats. The mechanistic activity of EPM was tested on various gastric-ulcer parameters, namely gastric pH, volume, acid-pepsin output, DNA content, histamine level, H(+)K(+)-ATPase activity, mucus content, microvascular permeability, antioxidant markers, and gastric-histopathological study. RESULTS: EPM significantly reduces the ulcer score against all the four tested gastric-ulcer models. In the PL model, EPM showed significant reduction (p < 0.05) in acid-pepsin output and cell shedding; however, no significant effect was observed on gastric volume, cell proliferation, stomach glandular weight, and histamine levels. EPM (400 mg/kg, p.o.) when compared with ulcer control showed significant increase in gastric pH by 41.6% and decrease in H(+)K(+)-ATPase activity by 47.73%. In addition, EPM showed significant increase in mucus content by 58.60% and a decrease in the microvascular permeability of Evans Blue by 85.00%, justifying its protective effects. Furthermore, EPM also showed significant antioxidant activity and histopathologically possessed excellent cytoprotective effect. CONCLUSION: The gastro-protective effect of EPM is attributed mainly to the defensive mechanism owing to the presence of a good quantity of polyphenolic components. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/25026332/Gastroprotective_activity_of_polyphenolic_rich_extract_of_Potentilla_mooniana_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2014.905794 DB - PRIME DP - Unbound Medicine ER -