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Dentin sialophosphoprotein: a regulatory protein for dental pulp stem cell identity and fate.
Stem Cells Dev. 2014 Dec 01; 23(23):2883-94.SC

Abstract

The dentin sialophosphoprotein (dspp) transcript is expressed during tooth development as a DSPP precursor protein, which then undergoes cleavage to form mature dentin sialoprotein (DSP) and phosphophoryn (PP) proteins. Previous studies using DSPP-knockout (KO) mice have reported that these animals have hypomineralized teeth, thin dentin, and a large dental pulp chamber, similar to those from patients with dentinogenesis imperfecta III. However, there is no information about factors that regulate dental pulp stem cell lineage fate, a critical early event in the odontoblast-dentin mineralization scheme. To reveal the role of DSPP in odontoblast lineage differentiation during tooth development, we systematically examined teeth from wild-type (wt) and DSPP-KO C57BL/6 mice between the ages of postnatal day 1 and 3 months. We found developmental abnormalities not previously reported, such as circular dentin formation within dental pulp cells and altered odontoblast differentiation in DSPP-KO mice, even as early as 1 day after birth. Surprisingly, we also identified chondrocyte-like cells in the dental pulp from KO-mice teeth. Thus, these studies that compare wt and DSPP-KO mice suggest that the expression of DSPP precursor protein is required for normal odontoblast lineage differentiation and that the absence of DSPP allows dental pulp cells to differentiate into chondrocyte-like cells, which could negatively impact pulpal wound healing and tissue regeneration.

Authors+Show Affiliations

1 Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Michigan , Ann Arbor, Michigan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

25027178

Citation

Guo, Shiliang, et al. "Dentin Sialophosphoprotein: a Regulatory Protein for Dental Pulp Stem Cell Identity and Fate." Stem Cells and Development, vol. 23, no. 23, 2014, pp. 2883-94.
Guo S, Lim D, Dong Z, et al. Dentin sialophosphoprotein: a regulatory protein for dental pulp stem cell identity and fate. Stem Cells Dev. 2014;23(23):2883-94.
Guo, S., Lim, D., Dong, Z., Saunders, T. L., Ma, P. X., Marcelo, C. L., & Ritchie, H. H. (2014). Dentin sialophosphoprotein: a regulatory protein for dental pulp stem cell identity and fate. Stem Cells and Development, 23(23), 2883-94. https://doi.org/10.1089/scd.2014.0066
Guo S, et al. Dentin Sialophosphoprotein: a Regulatory Protein for Dental Pulp Stem Cell Identity and Fate. Stem Cells Dev. 2014 Dec 1;23(23):2883-94. PubMed PMID: 25027178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dentin sialophosphoprotein: a regulatory protein for dental pulp stem cell identity and fate. AU - Guo,Shiliang, AU - Lim,Dandrich, AU - Dong,Zhihong, AU - Saunders,Thomas L, AU - Ma,Peter X, AU - Marcelo,Cynthia L, AU - Ritchie,Helena H, Y1 - 2014/08/21/ PY - 2014/7/17/entrez PY - 2014/7/17/pubmed PY - 2015/7/15/medline SP - 2883 EP - 94 JF - Stem cells and development JO - Stem Cells Dev VL - 23 IS - 23 N2 - The dentin sialophosphoprotein (dspp) transcript is expressed during tooth development as a DSPP precursor protein, which then undergoes cleavage to form mature dentin sialoprotein (DSP) and phosphophoryn (PP) proteins. Previous studies using DSPP-knockout (KO) mice have reported that these animals have hypomineralized teeth, thin dentin, and a large dental pulp chamber, similar to those from patients with dentinogenesis imperfecta III. However, there is no information about factors that regulate dental pulp stem cell lineage fate, a critical early event in the odontoblast-dentin mineralization scheme. To reveal the role of DSPP in odontoblast lineage differentiation during tooth development, we systematically examined teeth from wild-type (wt) and DSPP-KO C57BL/6 mice between the ages of postnatal day 1 and 3 months. We found developmental abnormalities not previously reported, such as circular dentin formation within dental pulp cells and altered odontoblast differentiation in DSPP-KO mice, even as early as 1 day after birth. Surprisingly, we also identified chondrocyte-like cells in the dental pulp from KO-mice teeth. Thus, these studies that compare wt and DSPP-KO mice suggest that the expression of DSPP precursor protein is required for normal odontoblast lineage differentiation and that the absence of DSPP allows dental pulp cells to differentiate into chondrocyte-like cells, which could negatively impact pulpal wound healing and tissue regeneration. SN - 1557-8534 UR - https://www.unboundmedicine.com/medline/citation/25027178/Dentin_sialophosphoprotein:_a_regulatory_protein_for_dental_pulp_stem_cell_identity_and_fate_ L2 - https://www.liebertpub.com/doi/10.1089/scd.2014.0066?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -