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Tetanus toxoids loaded glucomannosylated chitosan based nanohoming vaccine adjuvant with improved oral stability and immunostimulatory response.
Pharm Res. 2015 Jan; 32(1):122-34.PR

Abstract

PURPOSE

The present report embarks on rational designing of stable and functionalized chitosan nanoparticles for oral mucosal immunization.

METHODS

Stable glucomannosylated sCh-GM-NPs were prepared by tandem cross linking method followed by lyophilization. The in vitro stability of antigen and formulation, cellular uptake and immunostimulatory response were assessed by suitable experimental protocol.

RESULTS

Stability testing ensured the chemical and conformation permanency of encapsulated TT as well as robustness of sCh-GM-NPs in simulated biological media. The antigen release from sCh-GM-NPs followed initial burst followed by controlled Weibull's type of release profile. The higher intracellular uptake of sCh-GM-NPs in Raw 264.7 and Caco-2 was concentration and time dependent which mainly attributed to Clathrin and receptor mediated endocytosis via mannose and glucose receptor. The in vivo evaluation in animals revealed that sCh-GM-NPs posed significantly (p < 0.001) higher humoral, mucosal and cellular immune response than other counterparts. More importantly, commercial TT vaccine administered through oral or intramuscular route was unable to elicit all type of immune response.

CONCLUSION

The sCh-GM-NPs could be considered as promising vaccine adjuvant for oral tetanus immunization. Additionally, this technology expected to benefit the design and development of stable peroral formulation for administration of protein, peptides and variety of other antigens.

Authors+Show Affiliations

Centre for Pharmaceutical Nanotechnology, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, SAS Nagar, Punjab, 160 062, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25030184

Citation

Harde, Harshad, et al. "Tetanus Toxoids Loaded Glucomannosylated Chitosan Based Nanohoming Vaccine Adjuvant With Improved Oral Stability and Immunostimulatory Response." Pharmaceutical Research, vol. 32, no. 1, 2015, pp. 122-34.
Harde H, Agrawal AK, Jain S. Tetanus toxoids loaded glucomannosylated chitosan based nanohoming vaccine adjuvant with improved oral stability and immunostimulatory response. Pharm Res. 2015;32(1):122-34.
Harde, H., Agrawal, A. K., & Jain, S. (2015). Tetanus toxoids loaded glucomannosylated chitosan based nanohoming vaccine adjuvant with improved oral stability and immunostimulatory response. Pharmaceutical Research, 32(1), 122-34. https://doi.org/10.1007/s11095-014-1449-5
Harde H, Agrawal AK, Jain S. Tetanus Toxoids Loaded Glucomannosylated Chitosan Based Nanohoming Vaccine Adjuvant With Improved Oral Stability and Immunostimulatory Response. Pharm Res. 2015;32(1):122-34. PubMed PMID: 25030184.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tetanus toxoids loaded glucomannosylated chitosan based nanohoming vaccine adjuvant with improved oral stability and immunostimulatory response. AU - Harde,Harshad, AU - Agrawal,Ashish Kumar, AU - Jain,Sanyog, Y1 - 2014/07/17/ PY - 2014/04/18/received PY - 2014/07/02/accepted PY - 2014/7/18/entrez PY - 2014/7/18/pubmed PY - 2015/9/19/medline SP - 122 EP - 34 JF - Pharmaceutical research JO - Pharm Res VL - 32 IS - 1 N2 - PURPOSE: The present report embarks on rational designing of stable and functionalized chitosan nanoparticles for oral mucosal immunization. METHODS: Stable glucomannosylated sCh-GM-NPs were prepared by tandem cross linking method followed by lyophilization. The in vitro stability of antigen and formulation, cellular uptake and immunostimulatory response were assessed by suitable experimental protocol. RESULTS: Stability testing ensured the chemical and conformation permanency of encapsulated TT as well as robustness of sCh-GM-NPs in simulated biological media. The antigen release from sCh-GM-NPs followed initial burst followed by controlled Weibull's type of release profile. The higher intracellular uptake of sCh-GM-NPs in Raw 264.7 and Caco-2 was concentration and time dependent which mainly attributed to Clathrin and receptor mediated endocytosis via mannose and glucose receptor. The in vivo evaluation in animals revealed that sCh-GM-NPs posed significantly (p < 0.001) higher humoral, mucosal and cellular immune response than other counterparts. More importantly, commercial TT vaccine administered through oral or intramuscular route was unable to elicit all type of immune response. CONCLUSION: The sCh-GM-NPs could be considered as promising vaccine adjuvant for oral tetanus immunization. Additionally, this technology expected to benefit the design and development of stable peroral formulation for administration of protein, peptides and variety of other antigens. SN - 1573-904X UR - https://www.unboundmedicine.com/medline/citation/25030184/Tetanus_toxoids_loaded_glucomannosylated_chitosan_based_nanohoming_vaccine_adjuvant_with_improved_oral_stability_and_immunostimulatory_response_ L2 - https://doi.org/10.1007/s11095-014-1449-5 DB - PRIME DP - Unbound Medicine ER -