Comparison of hydrodynamically closed isotachophoresis-capillary zone electrophoresis with hydrodynamically open capillary zone electrophoresis hyphenated with tandem mass spectrometry in drug analysis: pheniramine, its metabolite and phenylephrine in human urine.
J Chromatogr A. 2014 Sep 05; 1358:285-92.JC

Abstract

The advanced two dimensional isotachophoresis (ITP)-capillary zone electrophoresis (CZE) hyphenated with tandem mass spectrometry (MS/MS, here triple quadrupole, QqQ) was developed in this work to demonstrate analytical potentialities of this approach in the analysis of drugs in multicomponent ionic matrices. Pheniramine (PHM), phenylephrine (PHE), paracetamol (PCM) and their potential metabolic products were taken for the analysis by the ITP-CZE-ESI-QqQ technique working in hydrodynamically closed CE separation system and then a comparison with the conventional (hydrodynamically open) CZE-ESI-QqQ technique was made. The ITP-CZE-ESI-QqQ method was favorable in terms of obtainable selectivity (due to highly effective heart-cut analysis), concentration limits of detection (LOD at pgmL(-1) levels due to enhanced sample load capacity and ITP preconcentration), sample handling (on-line sample pretreatment, i.e. clean-up, preconcentration, preseparation), and, by that, possibilities for future automation and miniaturization. On the other hand, this experimental arrangement, in contrast to the CZE-ESI-QqQ arrangement supported by an electroosmotic flow, is principally limited to the analysis of uniformly (i.e. positively or negatively) charged analytes in one run without any possibilities to analyze neutral compounds (here, PCM and neutral or acidic metabolites of the drugs had to be excluded from the analysis). Hence, these general characteristics should be considered when choosing a proper analytical CE-MS approach for a given biomedical application. Here, the analytical potential of the ITP-CZE-ESI-QqQ method was demonstrated showing the real time profiles of excreted targeted drugs and metabolite (PHM, PHE, M-PHM) in human urine after the administration of one dose of Theraflu(®) to the volunteers.

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Authors+Show Affiliations

Piešťanský J

Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, Odbojárov 10, SK-832 32 Bratislava, Slovak Republic; Toxicological and Antidoping Center, Faculty of Pharmacy, Comenius University, Odbojárov 10, SK-832 32 Bratislava, Slovak Republic.

Maráková K

Kovaľ M

Villa Labeco spol. s r. o., Chrapčiakova 1, 052 01 Spišská Nová Ves, Slovak Republic.

Mikuš P

MeSH

AcetaminophenElectroosmosisElectrophoresis, CapillaryHumansHydrodynamicsIsotachophoresisLimit of DetectionPheniraminePhenylephrineReference StandardsSpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryUrinalysis

Pub Type(s)

Comparative Study

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25035234

Citation

Piešťanský, Juraj, et al. "Comparison of Hydrodynamically Closed Isotachophoresis-capillary Zone Electrophoresis With Hydrodynamically Open Capillary Zone Electrophoresis Hyphenated With Tandem Mass Spectrometry in Drug Analysis: Pheniramine, Its Metabolite and Phenylephrine in Human Urine." Journal of Chromatography. A, vol. 1358, 2014, pp. 285-92.

Piešťanský J, Maráková K, Kovaľ M, et al. Comparison of hydrodynamically closed isotachophoresis-capillary zone electrophoresis with hydrodynamically open capillary zone electrophoresis hyphenated with tandem mass spectrometry in drug analysis: pheniramine, its metabolite and phenylephrine in human urine. J Chromatogr A. 2014;1358:285-92.

Piešťanský, J., Maráková, K., Kovaľ, M., & Mikuš, P. (2014). Comparison of hydrodynamically closed isotachophoresis-capillary zone electrophoresis with hydrodynamically open capillary zone electrophoresis hyphenated with tandem mass spectrometry in drug analysis: pheniramine, its metabolite and phenylephrine in human urine. Journal of Chromatography. A, 1358, 285-92. https://doi.org/10.1016/j.chroma.2014.06.083

Piešťanský J, et al. Comparison of Hydrodynamically Closed Isotachophoresis-capillary Zone Electrophoresis With Hydrodynamically Open Capillary Zone Electrophoresis Hyphenated With Tandem Mass Spectrometry in Drug Analysis: Pheniramine, Its Metabolite and Phenylephrine in Human Urine. J Chromatogr A. 2014 Sep 5;1358:285-92. PubMed PMID: 25035234.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Comparison of hydrodynamically closed isotachophoresis-capillary zone electrophoresis with hydrodynamically open capillary zone electrophoresis hyphenated with tandem mass spectrometry in drug analysis: pheniramine, its metabolite and phenylephrine in human urine. AU - Piešťanský,Juraj, AU - Maráková,Katarína, AU - Kovaľ,Marián, AU - Mikuš,Peter, Y1 - 2014/07/01/ PY - 2014/02/27/received PY - 2014/06/04/revised PY - 2014/06/25/accepted PY - 2014/7/19/entrez PY - 2014/7/19/pubmed PY - 2014/10/11/medline KW - Capillary isotachophoresis KW - Capillary zone electrophoresis KW - Human urine KW - Hyphenation KW - Multi drug analysis KW - Tandem mass spectrometry SP - 285 EP - 92 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1358 N2 - The advanced two dimensional isotachophoresis (ITP)-capillary zone electrophoresis (CZE) hyphenated with tandem mass spectrometry (MS/MS, here triple quadrupole, QqQ) was developed in this work to demonstrate analytical potentialities of this approach in the analysis of drugs in multicomponent ionic matrices. Pheniramine (PHM), phenylephrine (PHE), paracetamol (PCM) and their potential metabolic products were taken for the analysis by the ITP-CZE-ESI-QqQ technique working in hydrodynamically closed CE separation system and then a comparison with the conventional (hydrodynamically open) CZE-ESI-QqQ technique was made. The ITP-CZE-ESI-QqQ method was favorable in terms of obtainable selectivity (due to highly effective heart-cut analysis), concentration limits of detection (LOD at pgmL(-1) levels due to enhanced sample load capacity and ITP preconcentration), sample handling (on-line sample pretreatment, i.e. clean-up, preconcentration, preseparation), and, by that, possibilities for future automation and miniaturization. On the other hand, this experimental arrangement, in contrast to the CZE-ESI-QqQ arrangement supported by an electroosmotic flow, is principally limited to the analysis of uniformly (i.e. positively or negatively) charged analytes in one run without any possibilities to analyze neutral compounds (here, PCM and neutral or acidic metabolites of the drugs had to be excluded from the analysis). Hence, these general characteristics should be considered when choosing a proper analytical CE-MS approach for a given biomedical application. Here, the analytical potential of the ITP-CZE-ESI-QqQ method was demonstrated showing the real time profiles of excreted targeted drugs and metabolite (PHM, PHE, M-PHM) in human urine after the administration of one dose of Theraflu(®) to the volunteers. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/25035234/Comparison_of_hydrodynamically_closed_isotachophoresis_capillary_zone_electrophoresis_with_hydrodynamically_open_capillary_zone_electrophoresis_hyphenated_with_tandem_mass_spectrometry_in_drug_analysis:_pheniramine_its_metabolite_and_phenylephrine_in_human_urine_ DB - PRIME DP - Unbound Medicine ER -

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