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RP-HPLC and UV Spectrophotometric Methods for Estimation of Pirfenidone in Pharmaceutical Formulations.
Indian J Pharm Sci. 2014 May; 76(3):225-9.IJ

Abstract

High-performance liquid chromatographic and UV spectrophotometric methods were developed and validated for the quantitative determination of pirfenidone, a novel antifibrotic agent used in idiopathic pulmonary fibrosis. Chromatography was carried out by isocratic technique on a reversed-phase C18 Zorbax Eclipse plus column with mobile phase consisting of acetonitrile:water (35:65 %v/v) at flow rate of 0.7 ml/min. The UV spectrophotometric determinations were performed at 317 nm using methanol as a solvent. The proposed methods were validated according to International Conference on Harmonization ICH Q2 (R1) guidelines. The linearity range for pirfenidone was 0.2-5.0 and 3-25 μg/ml for HPLC and UV method, respectively. Both the methods were accurate and precise with recoveries in the range of 98 and 102 % and relative standard deviation <2 %. The developed methods were successfully applied for determination of pirfenidone in tablets.

Authors+Show Affiliations

Department of Pharmaceutical Sciences, Sardar Patel University, Vallabh Vidyanagar-388120, India.Department of Pharmaceutical Sciences, Sardar Patel University, Vallabh Vidyanagar-388120, India.Department of Pharmaceutical Sciences, Sardar Patel University, Vallabh Vidyanagar-388120, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25035534

Citation

Parmar, V K., et al. "RP-HPLC and UV Spectrophotometric Methods for Estimation of Pirfenidone in Pharmaceutical Formulations." Indian Journal of Pharmaceutical Sciences, vol. 76, no. 3, 2014, pp. 225-9.
Parmar VK, Desai SB, Vaja T. RP-HPLC and UV Spectrophotometric Methods for Estimation of Pirfenidone in Pharmaceutical Formulations. Indian J Pharm Sci. 2014;76(3):225-9.
Parmar, V. K., Desai, S. B., & Vaja, T. (2014). RP-HPLC and UV Spectrophotometric Methods for Estimation of Pirfenidone in Pharmaceutical Formulations. Indian Journal of Pharmaceutical Sciences, 76(3), 225-9.
Parmar VK, Desai SB, Vaja T. RP-HPLC and UV Spectrophotometric Methods for Estimation of Pirfenidone in Pharmaceutical Formulations. Indian J Pharm Sci. 2014;76(3):225-9. PubMed PMID: 25035534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RP-HPLC and UV Spectrophotometric Methods for Estimation of Pirfenidone in Pharmaceutical Formulations. AU - Parmar,V K, AU - Desai,S B, AU - Vaja,T, PY - 2013/06/03/received PY - 2014/03/16/revised PY - 2014/03/23/accepted PY - 2014/7/19/entrez PY - 2014/7/19/pubmed PY - 2014/7/19/medline KW - Pharmaceutical formulation KW - RP-HPLC KW - UV KW - pirfenidone SP - 225 EP - 9 JF - Indian journal of pharmaceutical sciences JO - Indian J Pharm Sci VL - 76 IS - 3 N2 - High-performance liquid chromatographic and UV spectrophotometric methods were developed and validated for the quantitative determination of pirfenidone, a novel antifibrotic agent used in idiopathic pulmonary fibrosis. Chromatography was carried out by isocratic technique on a reversed-phase C18 Zorbax Eclipse plus column with mobile phase consisting of acetonitrile:water (35:65 %v/v) at flow rate of 0.7 ml/min. The UV spectrophotometric determinations were performed at 317 nm using methanol as a solvent. The proposed methods were validated according to International Conference on Harmonization ICH Q2 (R1) guidelines. The linearity range for pirfenidone was 0.2-5.0 and 3-25 μg/ml for HPLC and UV method, respectively. Both the methods were accurate and precise with recoveries in the range of 98 and 102 % and relative standard deviation <2 %. The developed methods were successfully applied for determination of pirfenidone in tablets. SN - 0250-474X UR - https://www.unboundmedicine.com/medline/citation/25035534/RP_HPLC_and_UV_Spectrophotometric_Methods_for_Estimation_of_Pirfenidone_in_Pharmaceutical_Formulations_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25035534/ DB - PRIME DP - Unbound Medicine ER -
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