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Xylose and xylose/glucose co-fermentation by recombinant Saccharomyces cerevisiae strains expressing individual hexose transporters.
Enzyme Microb Technol. 2014 Sep; 63:13-20.EM

Abstract

Since the uptake of xylose is believed to be one of the rate-limiting steps for xylose ethanol fermentation by recombinant Saccharomyces cerevisiae strains, we transformed a hxt-null strain lacking the major hexose transporters (hxt1Δ-hxt7Δ and gal2Δ) with an integrative plasmid to overexpress the genes for xylose reductase (XYL1), xylitol dehydrogenase (XYL2) and xylulokinase (XKS1), and analyzed the impact that overexpression of the HXT1, HXT2, HXT5 or HXT7 permeases have in anaerobic batch fermentations using xylose, glucose, or xylose plus glucose as carbon sources. Our results revealed that the low-affinity HXT1 permease allowed the maximal consumption of sugars and ethanol production rates during xylose/glucose co-fermentations, but was incapable to allow xylose uptake when this sugar was the only carbon source. The moderately high-affinity HXT5 permease was a poor glucose transporter, and it also did not allow significant xylose uptake by the cells. The moderately high-affinity HXT2 permease allowed xylose uptake with the same rates as those observed during glucose consumption, even under co-fermentation conditions, but had the drawback of producing incomplete fermentations. Finally, the high-affinity HXT7 permease allowed efficient xylose fermentation, but during xylose/glucose co-fermentations this permease showed a clear preference for glucose. Thus, our results indicate that approaches to engineer S. cerevisiae HXT transporters to improve second generation bioethanol production need to consider the composition of the biomass sugar syrup, whereby the HXT1 transporter seems more suitable for hydrolysates containing xylose/glucose blends, whereas the HXT7 permease would be a better choice for xylose-enriched sugar streams.

Authors+Show Affiliations

Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, Brazil.Biomass Refinery Research Center (BRRC), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-hiroshima, Hiroshima 739-0046, Japan. Electronic address: a-matsushika@aist.go.jp.Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, Brazil.Biomass Refinery Research Center (BRRC), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-hiroshima, Hiroshima 739-0046, Japan.Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-909, Brazil.Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, Brazil. Electronic address: boris.stambuk@ufsc.br.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25039054

Citation

Gonçalves, Davi L., et al. "Xylose and Xylose/glucose Co-fermentation By Recombinant Saccharomyces Cerevisiae Strains Expressing Individual Hexose Transporters." Enzyme and Microbial Technology, vol. 63, 2014, pp. 13-20.
Gonçalves DL, Matsushika A, de Sales BB, et al. Xylose and xylose/glucose co-fermentation by recombinant Saccharomyces cerevisiae strains expressing individual hexose transporters. Enzyme Microb Technol. 2014;63:13-20.
Gonçalves, D. L., Matsushika, A., de Sales, B. B., Goshima, T., Bon, E. P., & Stambuk, B. U. (2014). Xylose and xylose/glucose co-fermentation by recombinant Saccharomyces cerevisiae strains expressing individual hexose transporters. Enzyme and Microbial Technology, 63, 13-20. https://doi.org/10.1016/j.enzmictec.2014.05.003
Gonçalves DL, et al. Xylose and Xylose/glucose Co-fermentation By Recombinant Saccharomyces Cerevisiae Strains Expressing Individual Hexose Transporters. Enzyme Microb Technol. 2014;63:13-20. PubMed PMID: 25039054.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Xylose and xylose/glucose co-fermentation by recombinant Saccharomyces cerevisiae strains expressing individual hexose transporters. AU - Gonçalves,Davi L, AU - Matsushika,Akinori, AU - de Sales,Belisa B, AU - Goshima,Tetsuya, AU - Bon,Elba P S, AU - Stambuk,Boris U, Y1 - 2014/05/17/ PY - 2014/03/19/received PY - 2014/05/08/revised PY - 2014/05/09/accepted PY - 2014/7/21/entrez PY - 2014/7/21/pubmed PY - 2015/4/7/medline KW - Bioethanol KW - Glucose/xylose co-fermentation KW - HXT transporters KW - Saccharomyces cerevisiae KW - Xylose fermentation SP - 13 EP - 20 JF - Enzyme and microbial technology JO - Enzyme Microb Technol VL - 63 N2 - Since the uptake of xylose is believed to be one of the rate-limiting steps for xylose ethanol fermentation by recombinant Saccharomyces cerevisiae strains, we transformed a hxt-null strain lacking the major hexose transporters (hxt1Δ-hxt7Δ and gal2Δ) with an integrative plasmid to overexpress the genes for xylose reductase (XYL1), xylitol dehydrogenase (XYL2) and xylulokinase (XKS1), and analyzed the impact that overexpression of the HXT1, HXT2, HXT5 or HXT7 permeases have in anaerobic batch fermentations using xylose, glucose, or xylose plus glucose as carbon sources. Our results revealed that the low-affinity HXT1 permease allowed the maximal consumption of sugars and ethanol production rates during xylose/glucose co-fermentations, but was incapable to allow xylose uptake when this sugar was the only carbon source. The moderately high-affinity HXT5 permease was a poor glucose transporter, and it also did not allow significant xylose uptake by the cells. The moderately high-affinity HXT2 permease allowed xylose uptake with the same rates as those observed during glucose consumption, even under co-fermentation conditions, but had the drawback of producing incomplete fermentations. Finally, the high-affinity HXT7 permease allowed efficient xylose fermentation, but during xylose/glucose co-fermentations this permease showed a clear preference for glucose. Thus, our results indicate that approaches to engineer S. cerevisiae HXT transporters to improve second generation bioethanol production need to consider the composition of the biomass sugar syrup, whereby the HXT1 transporter seems more suitable for hydrolysates containing xylose/glucose blends, whereas the HXT7 permease would be a better choice for xylose-enriched sugar streams. SN - 1879-0909 UR - https://www.unboundmedicine.com/medline/citation/25039054/Xylose_and_xylose/glucose_co_fermentation_by_recombinant_Saccharomyces_cerevisiae_strains_expressing_individual_hexose_transporters_ DB - PRIME DP - Unbound Medicine ER -