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Identification of phenolic acids and flavonoids in monofloral honey from Bangladesh by high performance liquid chromatography: determination of antioxidant capacity.
Biomed Res Int. 2014; 2014:737490.BR

Abstract

The aim of the present study was to characterize the phenolic acids, flavonoids, and antioxidant properties of monofloral honey collected from five different districts in Bangladesh. A new high performance liquid chromatography (HPLC) equipped with a UV detector method was developed for the identification of the phenolic acids and flavonoids. A total of five different phenolic acids were identified, with the most abundant being caffeic acid, benzoic acid, gallic acid, followed by chlorogenic acid and trans-cinnamic acid. The flavonoids, kaempferol, and catechin were most abundant, followed by myricetin and naringenin. The mean moisture content, total sugar content, and color characteristics of the honey samples were 18.36 ± 0.95%, 67.40 ± 5.63 g/100 g, and 129.27 ± 34.66 mm Pfund, respectively. The mean total phenolic acids, total flavonoid content, and proline content were 199.20 ± 135.23, 46.73 ± 34.16, and 556.40 ± 376.86 mg/kg, respectively, while the mean FRAP values and DPPH radical scavenging activity were 327.30 ± 231.87 μM Fe (II)/100 g and 36.95 ± 20.53%, respectively. Among the different types of honey, kalijira exhibited the highest phenolics and antioxidant properties. Overall, our study confirms that all the investigated honey samples are good sources of phenolic acids and flavonoids with good antioxidant properties.

Authors+Show Affiliations

Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia ; Faculty of Agro Based Industry, Universiti Malaysia Kelantan, Jeli Campus, Locked Bag No. 100, 17600 Jeli, Kelantan, Malaysia.Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh.Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25045696

Citation

Moniruzzaman, Mohammed, et al. "Identification of Phenolic Acids and Flavonoids in Monofloral Honey From Bangladesh By High Performance Liquid Chromatography: Determination of Antioxidant Capacity." BioMed Research International, vol. 2014, 2014, p. 737490.
Moniruzzaman M, Yung An C, Rao PV, et al. Identification of phenolic acids and flavonoids in monofloral honey from Bangladesh by high performance liquid chromatography: determination of antioxidant capacity. Biomed Res Int. 2014;2014:737490.
Moniruzzaman, M., Yung An, C., Rao, P. V., Hawlader, M. N., Azlan, S. A., Sulaiman, S. A., & Gan, S. H. (2014). Identification of phenolic acids and flavonoids in monofloral honey from Bangladesh by high performance liquid chromatography: determination of antioxidant capacity. BioMed Research International, 2014, 737490. https://doi.org/10.1155/2014/737490
Moniruzzaman M, et al. Identification of Phenolic Acids and Flavonoids in Monofloral Honey From Bangladesh By High Performance Liquid Chromatography: Determination of Antioxidant Capacity. Biomed Res Int. 2014;2014:737490. PubMed PMID: 25045696.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of phenolic acids and flavonoids in monofloral honey from Bangladesh by high performance liquid chromatography: determination of antioxidant capacity. AU - Moniruzzaman,Mohammed, AU - Yung An,Chua, AU - Rao,Pasupuleti Visweswara, AU - Hawlader,Mohammad Nurul Islam, AU - Azlan,Siti Amirah Binti Mohd, AU - Sulaiman,Siti Amrah, AU - Gan,Siew Hua, Y1 - 2014/06/24/ PY - 2014/03/03/received PY - 2014/05/15/revised PY - 2014/05/21/accepted PY - 2014/7/22/entrez PY - 2014/7/22/pubmed PY - 2015/3/13/medline SP - 737490 EP - 737490 JF - BioMed research international JO - Biomed Res Int VL - 2014 N2 - The aim of the present study was to characterize the phenolic acids, flavonoids, and antioxidant properties of monofloral honey collected from five different districts in Bangladesh. A new high performance liquid chromatography (HPLC) equipped with a UV detector method was developed for the identification of the phenolic acids and flavonoids. A total of five different phenolic acids were identified, with the most abundant being caffeic acid, benzoic acid, gallic acid, followed by chlorogenic acid and trans-cinnamic acid. The flavonoids, kaempferol, and catechin were most abundant, followed by myricetin and naringenin. The mean moisture content, total sugar content, and color characteristics of the honey samples were 18.36 ± 0.95%, 67.40 ± 5.63 g/100 g, and 129.27 ± 34.66 mm Pfund, respectively. The mean total phenolic acids, total flavonoid content, and proline content were 199.20 ± 135.23, 46.73 ± 34.16, and 556.40 ± 376.86 mg/kg, respectively, while the mean FRAP values and DPPH radical scavenging activity were 327.30 ± 231.87 μM Fe (II)/100 g and 36.95 ± 20.53%, respectively. Among the different types of honey, kalijira exhibited the highest phenolics and antioxidant properties. Overall, our study confirms that all the investigated honey samples are good sources of phenolic acids and flavonoids with good antioxidant properties. SN - 2314-6141 UR - https://www.unboundmedicine.com/medline/citation/25045696/Identification_of_phenolic_acids_and_flavonoids_in_monofloral_honey_from_Bangladesh_by_high_performance_liquid_chromatography:_determination_of_antioxidant_capacity_ L2 - https://dx.doi.org/10.1155/2014/737490 DB - PRIME DP - Unbound Medicine ER -