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Antioxidant activities of Lampaya medicinalis extracts and their main chemical constituents.
BMC Complement Altern Med. 2014 Jul 21; 14:259.BC

Abstract

BACKGROUND

Lampaya medicinalis Phil. (Verbenaceae) is a plant used by Aymara and Quechua ethnic groups from Northern Chile as folk medicine in the treatment and cure of various diseases. The aim of this study was to investigate the in vitro antioxidant activity, total phenols content, total flavonoids content, total antioxidant activity, reducing power, brine shrimp cytotoxicity and identify the principal chemical constituents.

METHODS

The crude hydroethanolic extract (HEE) and its partitioned fraction: hexane (HF), dichloromethane (DF), ethyl acetate (EAF), n-butanol (BF) and soluble residual aqueous fraction (RWF) were evaluated for their antioxidant activity using different assays namely, DPPH, ABTS, FRAP, β-carotene bleaching assay. The content of total phenolics and total flavonoids were measured by Folin-Ciocalteau and by the AlCl3 colorimetric method, respectively. Reducing power was determined by phosphomolybdate and hexacyanoferrate (III) methods. Biotoxicity assays were performed on shrimps of Artemia salina. The EAF was fractionated using chromatographic methods.

RESULTS

Considerable amount of phenolic and flavonoid contents were recorded in the hydroethanolic extract (HEE) and its derived fractions. Although HEE and all its derived fractions exhibited good antioxidant activities, the most distinguished radical scavenging potential was observed for ethyl acetate fraction (EAF). EAF showed the higher radical scavenging activity by DPPH (95%) and by ABTS (98%), antioxidant activity by FRAP (158.18 ± 5.79 mg equivalent Trolox/g fraction), β-carotene bleaching assay (86.8%), the highest total phenols content (101.26 ± 1.07 mg GAE/g fraction), the highest total flavonoids content (66.26 ± 3.31 μg quercetin/g fraction). The EAF extract showed an reducing power of 78% and 65% using the phosphomolybdate and hexacyanoferrate (III) assays, respectively.Four flavonoids, two p-hydroxyacetophenone derivatives and one iridoid were isolated from Lampaya medicinalis for the first time.

CONCLUSION

EtOAc soluble fraction (EAF) shows the strongest antioxidant activity, and it can be attributed to its high content in phenolic and flavonoid compounds. It can be concluded that L.medicinalis can be used as an effective natural source of antioxidant, as ethnomedicine and as a commercial basis for the development of nutraceuticals.

Authors+Show Affiliations

Laboratorio de Química Biológica, Instituto Antofagasta (IA), Universidad de Antofagasta, Antofagasta, Chile. glauco.morales@uantof.cl.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25047047

Citation

Morales, Glauco, and Adrián Paredes. "Antioxidant Activities of Lampaya Medicinalis Extracts and Their Main Chemical Constituents." BMC Complementary and Alternative Medicine, vol. 14, 2014, p. 259.
Morales G, Paredes A. Antioxidant activities of Lampaya medicinalis extracts and their main chemical constituents. BMC Complement Altern Med. 2014;14:259.
Morales, G., & Paredes, A. (2014). Antioxidant activities of Lampaya medicinalis extracts and their main chemical constituents. BMC Complementary and Alternative Medicine, 14, 259. https://doi.org/10.1186/1472-6882-14-259
Morales G, Paredes A. Antioxidant Activities of Lampaya Medicinalis Extracts and Their Main Chemical Constituents. BMC Complement Altern Med. 2014 Jul 21;14:259. PubMed PMID: 25047047.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activities of Lampaya medicinalis extracts and their main chemical constituents. AU - Morales,Glauco, AU - Paredes,Adrián, Y1 - 2014/07/21/ PY - 2014/04/24/received PY - 2014/07/16/accepted PY - 2014/7/23/entrez PY - 2014/7/23/pubmed PY - 2014/10/21/medline SP - 259 EP - 259 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 14 N2 - BACKGROUND: Lampaya medicinalis Phil. (Verbenaceae) is a plant used by Aymara and Quechua ethnic groups from Northern Chile as folk medicine in the treatment and cure of various diseases. The aim of this study was to investigate the in vitro antioxidant activity, total phenols content, total flavonoids content, total antioxidant activity, reducing power, brine shrimp cytotoxicity and identify the principal chemical constituents. METHODS: The crude hydroethanolic extract (HEE) and its partitioned fraction: hexane (HF), dichloromethane (DF), ethyl acetate (EAF), n-butanol (BF) and soluble residual aqueous fraction (RWF) were evaluated for their antioxidant activity using different assays namely, DPPH, ABTS, FRAP, β-carotene bleaching assay. The content of total phenolics and total flavonoids were measured by Folin-Ciocalteau and by the AlCl3 colorimetric method, respectively. Reducing power was determined by phosphomolybdate and hexacyanoferrate (III) methods. Biotoxicity assays were performed on shrimps of Artemia salina. The EAF was fractionated using chromatographic methods. RESULTS: Considerable amount of phenolic and flavonoid contents were recorded in the hydroethanolic extract (HEE) and its derived fractions. Although HEE and all its derived fractions exhibited good antioxidant activities, the most distinguished radical scavenging potential was observed for ethyl acetate fraction (EAF). EAF showed the higher radical scavenging activity by DPPH (95%) and by ABTS (98%), antioxidant activity by FRAP (158.18 ± 5.79 mg equivalent Trolox/g fraction), β-carotene bleaching assay (86.8%), the highest total phenols content (101.26 ± 1.07 mg GAE/g fraction), the highest total flavonoids content (66.26 ± 3.31 μg quercetin/g fraction). The EAF extract showed an reducing power of 78% and 65% using the phosphomolybdate and hexacyanoferrate (III) assays, respectively.Four flavonoids, two p-hydroxyacetophenone derivatives and one iridoid were isolated from Lampaya medicinalis for the first time. CONCLUSION: EtOAc soluble fraction (EAF) shows the strongest antioxidant activity, and it can be attributed to its high content in phenolic and flavonoid compounds. It can be concluded that L.medicinalis can be used as an effective natural source of antioxidant, as ethnomedicine and as a commercial basis for the development of nutraceuticals. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/25047047/Antioxidant_activities_of_Lampaya_medicinalis_extracts_and_their_main_chemical_constituents_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-14-259 DB - PRIME DP - Unbound Medicine ER -